Library Protocol: Lab specific protocol that may cover a number of steps in an experiment. Most typically this identifies methods for building a DNA or RNA library.
This kind of library was made from RNA < 200 nucleotides that was pre-treated sequentially with Calf Intestinal Alkaline Phosphatase followed by Tobacco Acid Pyrophosphatase. Hence, it will be enriched for capped RNAs.
This kind of library was made from RNA < 200 nucleotides that were pre-treated with Tobacco Acid Pyrophosphatase to remove any 5' caps that would preclude cloning. Hence, both capped and 5' monophosphate RNAs will be cloned.
Tobacco Acid Pyrophosphatase Only
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
biorupter, PCR 1-round
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
sonicator, PCR 1-round
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
ChIP AMpure XP
Faster ChIP protocol & gel size selection for ChIP-seq (Myers)