Human Gene HAO2 (R10971)
  Description: hydroxyacid oxidase 2
RefSeq Summary (NM_001005783): This gene is one of three related genes that have 2-hydroxyacid oxidase activity. The encoded protein localizes to the peroxisome has the highest activity toward the substrate 2-hydroxypalmitate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].
Transcript (Including UTRs)
   Position: hg17 chr1:119,623,444-119,648,793 Size: 25,350 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg17 chr1:119,635,751-119,648,505 Size: 12,755 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2005-06-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:119,623,444-119,648,793)mRNA (may differ from genome)Protein (351 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCHPRDMGIOMIMPubMed
ReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HAOX2_HUMAN
DESCRIPTION: RecName: Full=Hydroxyacid oxidase 2; Short=HAOX2; EC=1.1.3.15; AltName: Full=(S)-2-hydroxy-acid oxidase, peroxisomal; AltName: Full=Cell growth-inhibiting gene 16 protein; AltName: Full=Long chain alpha-hydroxy acid oxidase; AltName: Full=Long-chain L-2-hydroxy acid oxidase;
FUNCTION: Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of L-alpha-amino acids.
CATALYTIC ACTIVITY: (S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).
COFACTOR: FMN.
SUBUNIT: Homotetramer or homooctamer (By similarity).
SUBCELLULAR LOCATION: Peroxisome.
TISSUE SPECIFICITY: Liver and kidney.
SIMILARITY: Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
SIMILARITY: Contains 1 FMN hydroxy acid dehydrogenase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -104.90352-0.298 Picture PostScript Text
3' UTR -61.65288-0.214 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013785 - Aldolase_TIM
IPR012133 - Alpha-hydoxy_acid_DH_FMN
IPR000262 - FMN-dep_DH
IPR008259 - FMN_hydac_DH_AS

Pfam Domains:
PF01070 - FMN-dependent dehydrogenase

ModBase Predicted Comparative 3D Structure on Q9NYQ3
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologGenome BrowserGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
MGIRGDEnsemblFlyBaseWormBaseSGD
Protein Sequence  Protein SequenceProtein SequenceProtein Sequence
Alignment  AlignmentAlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0003973 (S)-2-hydroxy-acid oxidase activity
GO:0005102 receptor binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0052852 very-long-chain-(S)-2-hydroxy-acid oxidase activity
GO:0052853 long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity
GO:0052854 medium-chain-(S)-2-hydroxy-acid oxidase activity

Biological Process:
GO:0006625 protein targeting to peroxisome
GO:0009062 fatty acid catabolic process
GO:0019395 fatty acid oxidation
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005777 peroxisome
GO:0005782 peroxisomal matrix
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK298289 - Homo sapiens cDNA FLJ60761 complete cds, highly similar to Hydroxyacid oxidase 2 (EC 1.1.3.15).
BC020863 - Homo sapiens hydroxyacid oxidase 2 (long chain), mRNA (cDNA clone MGC:24062 IMAGE:4557798), complete cds.
AF231917 - Homo sapiens long-chain 2-hydroxy acid oxidase HAOX2 (HAOX2) mRNA, complete cds.
AF203975 - Homo sapiens long-chain L-2-hydroxy acid oxidase mRNA, complete cds.
AY513277 - Homo sapiens growth-inhibiting protein 16 (GIG16) mRNA, complete cds.
CU676752 - Synthetic construct Homo sapiens gateway clone IMAGE:100022240 5' read HAO2 mRNA.
DQ895163 - Synthetic construct Homo sapiens clone IMAGE:100009623; FLH181964.01L; RZPDo839B03137D hydroxyacid oxidase 2 (long chain) (HAO2) gene, encodes complete protein.
EU176321 - Synthetic construct Homo sapiens clone IMAGE:100006420; FLH181971.01X; RZPDo839D06251D hydroxyacid oxidase 2 (long chain) (HAO2) gene, encodes complete protein.
KJ898847 - Synthetic construct Homo sapiens clone ccsbBroadEn_08241 HAO2 gene, encodes complete protein.
JD020281 - Sequence 1305 from Patent EP1572962.
JD026973 - Sequence 7997 from Patent EP1572962.
JD341140 - Sequence 322164 from Patent EP1572962.
JD115338 - Sequence 96362 from Patent EP1572962.
JD282440 - Sequence 263464 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00630 - Glyoxylate and dicarboxylate metabolism
hsa01100 - Metabolic pathways
hsa04146 - Peroxisome

Reactome (by CSHL, EBI, and GO)

Protein Q9NYQ3 (Reactome details) participates in the following event(s):

R-HSA-9033233 PEX5S,L binds cargo proteins containing PTS1
R-HSA-6787811 HAO2 tetramer oxidises 2OH-PALM
R-HSA-9033236 PEX5S,L:Cargo binds PEX13:PEX14 of PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Complex)
R-HSA-9033241 Peroxisomal protein import
R-HSA-390918 Peroxisomal lipid metabolism
R-HSA-392499 Metabolism of proteins
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: AF231917, BC020863, HAOX2, NM_016527
UCSC ID: NM_001005783
RefSeq Accession: NM_001005783
Protein: Q9NYQ3 (aka HAOX2_HUMAN or HAO2_HUMAN)
CCDS: CCDS901.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.