Human Gene CYP2S1 (uc002opw.3)
  Description: Homo sapiens cytochrome P450, family 2, subfamily S, polypeptide 1 (CYP2S1), mRNA.
RefSeq Summary (NM_030622): This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr19:41,699,115-41,713,444 Size: 14,330 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg19 chr19:41,699,170-41,712,393 Size: 13,224 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:41,699,115-41,713,444)mRNA (may differ from genome)Protein (504 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CP2S1_HUMAN
DESCRIPTION: RecName: Full=Cytochrome P450 2S1; EC=1.14.14.1; AltName: Full=CYPIIS1;
FUNCTION: Has a potential importance for extrahepatic xenobiotic metabolism.
CATALYTIC ACTIVITY: RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: High level of expression in trachea, lung, stomach, small intestine, and spleen.
SIMILARITY: Belongs to the cytochrome P450 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): CYP2S1
CDC HuGE Published Literature: CYP2S1

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • D001564 Benzo(a)pyrene
  • D014212 Tretinoin
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • C425784 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • D015127 9,10-Dimethyl-1,2-benzanthracene
  • C023389 9,10-dihydrobenzo(a)pyrene
  • D000082 Acetaminophen
  • D016604 Aflatoxin B1
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.50 RPKM in Stomach
Total median expression: 93.42 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.2055-0.349 Picture PostScript Text
3' UTR -337.481051-0.321 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001128 - Cyt_P450
IPR017972 - Cyt_P450_CS
IPR002401 - Cyt_P450_E_grp-I
IPR008067 - Cyt_P450_E_grp-I_CYP2A-like

Pfam Domains:
PF00067 - Cytochrome P450

SCOP Domains:
48264 - Cytochrome P450

ModBase Predicted Comparative 3D Structure on Q96SQ9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0005515 protein binding
GO:0008392 arachidonic acid epoxygenase activity
GO:0008395 steroid hydroxylase activity
GO:0008401 retinoic acid 4-hydroxylase activity
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
GO:0070330 aromatase activity

Biological Process:
GO:0019373 epoxygenase P450 pathway
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0031090 organelle membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  AK027605 - Homo sapiens cDNA FLJ14699 fis, clone NT2RP2006571, moderately similar to CYTOCHROME P450 2G1 (EC 1.14.14.1).
AY358603 - Homo sapiens clone DNA76395 Cytochrome p450 (UNQ891) mRNA, complete cds.
AK296087 - Homo sapiens cDNA FLJ52866 complete cds, highly similar to Cytochrome P450 2S1 (EC 1.14.14.1).
BC033691 - Homo sapiens cytochrome P450, family 2, subfamily S, polypeptide 1, mRNA (cDNA clone MGC:44853 IMAGE:5212609), complete cds.
CU689700 - Synthetic construct Homo sapiens gateway clone IMAGE:100019707 5' read CYP2S1 mRNA.
DQ893363 - Synthetic construct clone IMAGE:100005993; FLH196613.01X; RZPDo839F10156D cytochrome P450, family 2, subfamily S, polypeptide 1 (CYP2S1) gene, encodes complete protein.
DQ896681 - Synthetic construct Homo sapiens clone IMAGE:100011141; FLH196609.01L; RZPDo839F10155D cytochrome P450, family 2, subfamily S, polypeptide 1 (CYP2S1) gene, encodes complete protein.
KJ898726 - Synthetic construct Homo sapiens clone ccsbBroadEn_08120 CYP2S1 gene, encodes complete protein.
AF335278 - Homo sapiens cytochrome P450 2S1 (CYP2S1) mRNA, complete cds.
JD222110 - Sequence 203134 from Patent EP1572962.
JD386503 - Sequence 367527 from Patent EP1572962.
JD096852 - Sequence 77876 from Patent EP1572962.
JD095714 - Sequence 76738 from Patent EP1572962.
JD424230 - Sequence 405254 from Patent EP1572962.
JD494067 - Sequence 475091 from Patent EP1572962.
JD049622 - Sequence 30646 from Patent EP1572962.
JD050715 - Sequence 31739 from Patent EP1572962.
JD537130 - Sequence 518154 from Patent EP1572962.
JD154170 - Sequence 135194 from Patent EP1572962.
JD432387 - Sequence 413411 from Patent EP1572962.
JD542427 - Sequence 523451 from Patent EP1572962.
JD211961 - Sequence 192985 from Patent EP1572962.
JD120712 - Sequence 101736 from Patent EP1572962.
JD351983 - Sequence 333007 from Patent EP1572962.
JD190893 - Sequence 171917 from Patent EP1572962.
JD334434 - Sequence 315458 from Patent EP1572962.
JD189534 - Sequence 170558 from Patent EP1572962.
JD392586 - Sequence 373610 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00980 - Metabolism of xenobiotics by cytochrome P450

BioCyc Knowledge Library
PWY66-201 - nicotine degradation II
PWY66-221 - nicotine degradation III
PWY66-241 - bupropion degradation

Reactome (by CSHL, EBI, and GO)

Protein Q96SQ9 (Reactome details) participates in the following event(s):

R-HSA-211874 CYP2S1 4-hydroxylates atRA
R-HSA-76354 Vinyl chloride is oxidized to 2-Chloroethylene oxide
R-HSA-76397 Acetaminophen oxidised to N-acetylbenzoquinoneimine (NAPQI)
R-HSA-76416 Benzene is hydroxylated to phenol
R-HSA-76434 Dehalogenation of carbon tetrachloride to form a free radical
R-HSA-76475 Dehalogenation of the poly-halogenated hydrocarbon Halothane to form the acylhalide Trifluoroacetlychloride and hydrogen bromide
R-HSA-143468 MEOS oxidizes ethanol to acetaldehyde
R-HSA-211910 CYP2C8 inactivates paclitaxel by 6alpha-hydroxylation
R-HSA-211988 CYP2C9 inactivates tolbutamide by 4methyl-hydroxylation
R-HSA-211991 Cyclophosphamide is 4-hydroxylated by CYP2B6
R-HSA-212005 CYP2F1 dehydrogenates 3-methylindole
R-HSA-212004 CYP2C18 initiates bioactivation of phenytoin by 4-hydroxylation
R-HSA-211929 CYP2C19 5-hydroxylates omeprazole
R-HSA-211958 Miscellaneous substrates
R-HSA-211999 CYP2E1 reactions
R-HSA-211981 Xenobiotics
R-HSA-211897 Cytochrome P450 - arranged by substrate type
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: CP2S1_HUMAN, NM_030622, NP_085125, Q96SQ9, Q9BZ66, UNQ891/PRO1906
UCSC ID: uc002opw.3
RefSeq Accession: NM_030622
Protein: Q96SQ9 (aka CP2S1_HUMAN or C2S1_HUMAN)
CCDS: CCDS12573.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_030622.6
exon count: 9CDS single in 3' UTR: no RNA size: 2657
ORF size: 1515CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2584.00frame shift in genome: no % Coverage: 98.65
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.