Human Gene DDX27 (uc002xuh.3) Description and Page Index
  Description: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), mRNA.
RefSeq Summary (NM_017895): DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017].
Transcript (Including UTRs)
   Position: hg19 chr20:47,835,832-47,860,614 Size: 24,783 Total Exon Count: 21 Strand: +
Coding Region
   Position: hg19 chr20:47,835,893-47,860,371 Size: 24,479 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr20:47,835,832-47,860,614)mRNA (may differ from genome)Protein (796 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedStanford SOURCETreefamUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Probable ATP-dependent RNA helicase DDX27; EC=; AltName: Full=DEAD box protein 27;
FUNCTION: Probable ATP-dependent RNA helicase.
CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
SIMILARITY: Contains 1 helicase ATP-binding domain.
SIMILARITY: Contains 1 helicase C-terminal domain.
CAUTION: It is uncertain whether Met-1 or Met-32 is the initiator.
SEQUENCE CAUTION: Sequence=AAF28937.1; Type=Frameshift; Positions=Several; Sequence=AAF28937.1; Type=Miscellaneous discrepancy; Note=Sequencing errors; Sequence=AAG22482.1; Type=Frameshift; Positions=208, 427, 434, 532; Sequence=AAH16060.2; Type=Erroneous initiation; Sequence=BAA91284.1; Type=Erroneous initiation; Sequence=BAB14343.1; Type=Erroneous initiation;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): DDX27
CDC HuGE Published Literature: DDX27
Positive Disease Associations: Attention Deficit Disorder with Hyperactivity , Iron
Related Studies:
  1. Attention Deficit Disorder with Hyperactivity
    , , . [PubMed 0]
  2. Iron
    , , . [PubMed 0]
  3. Iron
    , , . [PubMed 0]
           more ... click here to view the complete list

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.03 RPKM in Ovary
Total median expression: 498.46 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.0061-0.262 Picture PostScript Text
3' UTR -61.95243-0.255 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011545 - DNA/RNA_helicase_DEAD/DEAH_N
IPR014001 - Helicase_ATP-bd
IPR001650 - Helicase_C
IPR000629 - RNA-helicase_DEAD-box_CS
IPR014014 - RNA_helicase_DEAD_Q_motif

Pfam Domains:
PF00270 - DEAD/DEAH box helicase
PF00271 - Helicase conserved C-terminal domain
PF04851 - Type III restriction enzyme, res subunit

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on Q96GQ7
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene Details  Gene DetailsGene DetailsGene Details
Gene Sorter  Gene SorterGene SorterGene Sorter
  Protein SequenceProtein SequenceProtein SequenceProtein Sequence

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity

Biological Process:
GO:0006364 rRNA processing
GO:0010501 RNA secondary structure unwinding
GO:0042254 ribosome biogenesis

Cellular Component:
GO:0005634 nucleus
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005737 cytoplasm

-  Descriptions from all associated GenBank mRNAs
  BC126287 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:161565 IMAGE:8992003), complete cds.
BC130275 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:163147 IMAGE:40146306), complete cds.
BC144125 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:177662 IMAGE:9052645), complete cds.
HQ258508 - Synthetic construct Homo sapiens clone IMAGE:100072937 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27) gene, encodes complete protein.
KJ899168 - Synthetic construct Homo sapiens clone ccsbBroadEn_08562 DDX27 gene, encodes complete protein.
AY044431 - Homo sapiens RNA helicase-like protein mRNA, complete cds.
AL832131 - Homo sapiens mRNA; cDNA DKFZp686H1510 (from clone DKFZp686H1510).
EU718675 - Homo sapiens DEAD box polypeptide 27 (DDX27) mRNA, complete cds.
AK300135 - Homo sapiens cDNA FLJ61659 complete cds, highly similar to Probable ATP-dependent RNA helicase DDX27 (EC 3.6.1.-).
AK022979 - Homo sapiens cDNA FLJ12917 fis, clone NT2RP2004568, weakly similar to PUTATIVE ATP-DEPENDENT RNA HELICASE C30D11.03.
BC009304 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4128730), partial cds.
BC016060 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4903119), partial cds.
BC011927 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4544412), partial cds.
HQ258546 - Synthetic construct Homo sapiens clone IMAGE:100072975 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27) gene, encodes complete protein.
KJ906237 - Synthetic construct Homo sapiens clone ccsbBroadEn_15907 DDX27 gene, encodes complete protein.
AF193054 - Homo sapiens PP3241 mRNA, complete cds.
BC104480 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:6387875), partial cds.
AK025891 - Homo sapiens cDNA: FLJ22238 fis, clone HRC02173, highly similar to AF161377 Homo sapiens HSPC259 mRNA.
AF336851 - Homo sapiens RNA helicase-like protein (RHLP) mRNA, complete cds.
AK000603 - Homo sapiens cDNA FLJ20596 fis, clone KAT08049.
AK223116 - Homo sapiens mRNA for DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 variant, clone: KAT08049.
AK127128 - Homo sapiens cDNA FLJ45185 fis, clone BRAWH3048317, highly similar to ATP dependent RNA helicase.
AL512707 - Homo sapiens mRNA; cDNA DKFZp667N057 (from clone DKFZp667N057).
AF161377 - Homo sapiens HSPC259 mRNA, partial cds.
JD544308 - Sequence 525332 from Patent EP1572962.
JD104795 - Sequence 85819 from Patent EP1572962.
JD401366 - Sequence 382390 from Patent EP1572962.
JD548437 - Sequence 529461 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A0AVB6, B7ZLY1, DDX27_HUMAN, HSPC259, NM_017895, NP_060365, PP3241, Q5VXM7, Q8WYG4, Q969N7, Q96F57, Q96GQ7, Q96L97, Q9BWY9, Q9BXF0, Q9H990, Q9NWU3, Q9P0C2, Q9UGD6, RHLP
UCSC ID: uc002xuh.3
RefSeq Accession: NM_017895
Protein: Q96GQ7 (aka DDX27_HUMAN or DD27_HUMAN)
CCDS: CCDS13416.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_017895.7
exon count: 21CDS single in 3' UTR: no RNA size: 2711
ORF size: 2391CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4852.50frame shift in genome: no % Coverage: 99.41
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.