ID:T2FB_HUMAN DESCRIPTION: RecName: Full=General transcription factor IIF subunit 2; EC=3.6.4.12; AltName: Full=ATP-dependent helicase GTF2F2; AltName: Full=General transcription factor IIF 30 kDa subunit; AltName: Full=Transcription initiation factor IIF subunit beta; Short=TFIIF-beta; AltName: Full=Transcription initiation factor RAP30; FUNCTION: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1. INTERACTION: P46776:RPL27A; NbExp=2; IntAct=EBI-1030560, EBI-350581; Q8WW35:TCTEX1D2; NbExp=2; IntAct=EBI-1030560, EBI-2692044; O94763:URI1; NbExp=4; IntAct=EBI-1030560, EBI-357067; SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the TFIIF beta subunit family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P13984
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000398 mRNA splicing, via spliceosome GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0006368 transcription elongation from RNA polymerase II promoter GO:0006370 7-methylguanosine mRNA capping GO:0008543 fibroblast growth factor receptor signaling pathway GO:0016070 RNA metabolic process GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter GO:0042795 snRNA transcription from RNA polymerase II promoter GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
AK291545 - Homo sapiens cDNA FLJ75706 complete cds, highly similar to Homo sapiens general transcription factor IIF, polypeptide 2 (30kD subunit) (GTF2F2), mRNA. BC001771 - Homo sapiens general transcription factor IIF, polypeptide 2, 30kDa, mRNA (cDNA clone MGC:1502 IMAGE:3351446), complete cds. X16901 - Human mRNA for RAP30 subunit of transcription initiation factor RAP30/74. AB527978 - Synthetic construct DNA, clone: pF1KB7522, Homo sapiens GTF2F2 gene for general transcription factor IIF, polypeptide 2, 30kDa, without stop codon, in Flexi system. EU831971 - Synthetic construct Homo sapiens clone HAIB:100067000; DKFZo008F1223 general transcription factor IIF, polypeptide 2, 30kDa protein (GTF2F2) gene, encodes complete protein. EU832064 - Synthetic construct Homo sapiens clone HAIB:100067093; DKFZo004F1224 general transcription factor IIF, polypeptide 2, 30kDa protein (GTF2F2) gene, encodes complete protein. KJ896945 - Synthetic construct Homo sapiens clone ccsbBroadEn_06339 GTF2F2 gene, encodes complete protein. BT019525 - Homo sapiens general transcription factor IIF, polypeptide 2, 30kDa mRNA, complete cds. X59745 - H.sapiens RAP30 mRNA encoding RAP30. JD140468 - Sequence 121492 from Patent EP1572962. JD523667 - Sequence 504691 from Patent EP1572962. JD516445 - Sequence 497469 from Patent EP1572962. JD350803 - Sequence 331827 from Patent EP1572962. JD145827 - Sequence 126851 from Patent EP1572962. JD177972 - Sequence 158996 from Patent EP1572962. JD259069 - Sequence 240093 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa03022 - Basal transcription factors
Reactome (by CSHL, EBI, and GO)
Protein P13984 (Reactome details) participates in the following event(s):
R-HSA-6810234 General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene R-HSA-113429 Elongating transcript encounters a lesion in the template R-HSA-109638 Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex R-HSA-73946 Abortive initiation R-HSA-75856 Abortive Initiation Before Second Transition R-HSA-75891 Abortive Initiation After Second Transition R-HSA-167468 Abortive HIV-1 Initiation After Second Transition R-HSA-167474 Abortive HIV-1 Initiation Before Second Transition R-HSA-167477 Abortive HIV-1 initiation after formation of the first phosphodiester bond R-HSA-112395 Abortive termination of elongation after arrest R-HSA-167459 Abortive termination of HIV-1 elongation after arrest (Tat-containing elongation complex) R-HSA-167481 Abortive termination of HIV-1 elongation after arrest R-HSA-6810238 RNA polymerase II binds initiation factors at promoter of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-72103 Formation of pre-mRNPs R-HSA-75080 Formation of AT-AC A complex R-HSA-72095 Internal Methylation of mRNA R-HSA-75873 Addition of Nucleotides 5 through 9 on the growing Transcript R-HSA-76576 Addition of nucleotides 10 and 11 on the growing transcript: Third Transition R-HSA-111264 Addition of nucleotides between position +11 and +30 R-HSA-75864 Newly Formed Phosphodiester Bond Stabilized and PPi Released R-HSA-75866 Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP R-HSA-75095 Binding of TFIIE to the growing preinitiation complex R-HSA-75861 NTP Binds Active Site of RNA Polymerase II R-HSA-75862 Fall Back to Closed Pre-initiation Complex R-HSA-75949 RNA Polymerase II Promoter Opening: First Transition R-HSA-113430 Extrusion of 5'-end of 30 nt long transcript through the pore in Pol II complex R-HSA-77071 Phosphorylation (Ser5) of RNA pol II CTD R-HSA-167117 Addition of nucleotides 10 and 11 on the growing HIV-1 transcript: Third Transition R-HSA-167136 Addition of nucleotides 5 through 9 on the growing HIV-1 transcript R-HSA-167134 Newly formed phosphodiester bond stabilized and PPi released R-HSA-167098 Phosphorylation (Ser5) of RNA pol II CTD R-HSA-167111 Extrusion of 5'-end of 30 nt long HIV-1 transcript through the pore in Pol II complex R-HSA-167130 Nucleophillic attack by 3'-hydroxyl oxygen of nascent HIV-1 transcript on the Alpha phosphate of NTP R-HSA-167115 Addition of nucleotides between position +11 and +30 on HIV-1 transcript R-HSA-167118 NTP binds active site of RNA Polymerase II in HIV-1 open pre-initiation complex R-HSA-167484 Fall Back to Closed Pre-initiation Complex R-HSA-167097 HIV Promoter Opening: First Transition R-HSA-6803523 PTB and hnRNPA1 bind FGFR2 pre-mRNA to repress IIIb splicing and promote formation of FGFR2c mRNA R-HSA-6803527 ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and expression R-HSA-77068 Activation of GT R-HSA-77069 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-77073 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-77077 Capping complex formation R-HSA-156661 Formation of Exon Junction Complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-77095 Recognition and binding of the mRNA cap by the cap-binding complex R-HSA-77090 Methylation of GMP-cap by RNA Methyltransferase R-HSA-167089 Recognition and binding of the HIV-1 mRNA cap by the cap-binding complex R-HSA-167133 Activation of GT R-HSA-167128 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-167153 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-112379 Recruitment of elongation factors to form elongation complex R-HSA-112381 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-113411 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing R-HSA-113412 Pol II elongation complex moves on the template as transcript elongates R-HSA-113414 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest R-HSA-112385 Addition of nucleotides leads to transcript elongation R-HSA-112392 Resumption of elongation after recovery from pausing R-HSA-113413 TFIIS-mediated recovery of elongation from arrest R-HSA-112396 Separation of elongating transcript from template R-HSA-167150 Resumption of elongation of HIV-1 transcript after recovery from pausing R-HSA-167076 2-4 nt.backtracking of Pol II complex on the HIV-1 template leading to elongation pausing R-HSA-167077 Recruitment of elongation factors to form HIV-1 elongation complex R-HSA-167084 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-167282 2-4 nt.backtracking of Pol II complex on the HIV-1 template leading to elongation pausing R-HSA-167284 7-14 nt. Backtracking of Pol II complex on the HIV-1 template leading to elongation arrest R-HSA-167288 TFIIS-mediated recovery of HIV-1 elongation from arrest R-HSA-167292 Resumption of elongation of HIV-1 transcript after recovery from pausing R-HSA-167148 TFIIS-mediated recovery of elongation from arrest R-HSA-167090 7-14 nt. Backtracking of Pol II complex on the HIV-1 template leading to elongation arrest R-HSA-170706 Phosphorylation of NEFL by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167191 Hyperphosphorylation (Ser2) of RNA Pol II CTD by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167192 Pol II elongation complex moves on the HIV-1 template as transcript elongates R-HSA-167181 Addition of nucleotides leads to HIV-1 transcript elongation R-HSA-167197 Separation of elongating HIV-1 transcript from template R-HSA-170704 Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167196 Recruitment of elongation factors to form HIV-1 elongation complex R-HSA-9012319 p-TEFb phosphorylates serine 2 in RNA polymerase II CTD R-HSA-113407 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-112383 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-167083 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-167072 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-9012315 ESR1:ESTG:P-TEFb recruited to paused RNA polymerase II on MYB gene R-HSA-6810235 RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of heptad repeats in the C-terminal domain R-HSA-6810233 CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-75081 Formation of AT-AC B Complex R-HSA-75082 ATAC spliceosome mediated Lariat formation,5' splice site cleavage R-HSA-75079 Formation of AT-AC C complex R-HSA-75083 ATAC spliceosome mediated 3' splice site cleavage, exon ligation R-HSA-109639 Formation of the closed pre-initiation complex R-HSA-77078 Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme R-HSA-77081 Formation of the CE:GMP intermediate complex R-HSA-77085 Dissociation of transcript with 5'-GMP from GT R-HSA-77083 Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA R-HSA-6797616 CCNK:CDK12 binds RNA Pol II at DNA repair genes R-HSA-113402 Formation of DSIF:NELF:early elongation complex R-HSA-167085 Formation of DSIF:NELF:HIV-1 early elongation complex R-HSA-6797606 CDK12 phosphorylates RNA Pol II CTD at DNA repair genes R-HSA-113409 Abortive termination of early transcription elongation by DSIF:NELF R-HSA-167087 Limited elongation of the HIV-1 transcript R-HSA-167478 Abortive termination of HIV-1 early transcription elongation by DSIF:NELF R-HSA-167147 Separation of abortive HIV-1 transcript from template R-HSA-6807505 RNA polymerase II transcribes snRNA genes R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167161 HIV Transcription Initiation R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-167243 Tat-mediated HIV elongation arrest and recovery R-HSA-167287 HIV elongation arrest and recovery R-HSA-73857 RNA Polymerase II Transcription R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-72165 mRNA Splicing - Minor Pathway R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167172 Transcription of the HIV genome R-HSA-6803529 FGFR2 alternative splicing R-HSA-8851708 Signaling by FGFR2 IIIa TM R-HSA-72086 mRNA Capping R-HSA-167158 Formation of the HIV-1 Early Elongation Complex R-HSA-72172 mRNA Splicing R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167290 Pausing and recovery of HIV elongation R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript R-HSA-9018519 Estrogen-dependent gene expression R-HSA-113418 Formation of the Early Elongation Complex R-HSA-74160 Gene expression (Transcription) R-HSA-8953854 Metabolism of RNA R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-5654738 Signaling by FGFR2 R-HSA-1839126 FGFR2 mutant receptor activation R-HSA-167169 HIV Transcription Elongation R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes R-HSA-8939211 ESR-mediated signaling R-HSA-162587 HIV Life Cycle R-HSA-190236 Signaling by FGFR R-HSA-5655253 Signaling by FGFR2 in disease R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-9006931 Signaling by Nuclear Receptors R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-162906 HIV Infection R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-1226099 Signaling by FGFR in disease R-HSA-212436 Generic Transcription Pathway R-HSA-162582 Signal Transduction R-HSA-5663205 Infectious disease R-HSA-5663202 Diseases of signal transduction R-HSA-1643685 Disease
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Common Gene Haplotype Alleles 
