Human Gene NLN (uc003juf.3) Description and Page Index
  Description: Homo sapiens neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein, mRNA.
RefSeq Summary (NM_020726): This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun 2010]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB033052.2, SRR1803615.18418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000380985.10/ ENSP00000370372.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr5:65,018,023-65,125,111 Size: 107,089 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr5:65,018,201-65,118,743 Size: 100,543 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr5:65,018,023-65,125,111)mRNA (may differ from genome)Protein (704 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Neurolysin, mitochondrial; EC=; AltName: Full=Angiotensin-binding protein; AltName: Full=Microsomal endopeptidase; Short=MEP; AltName: Full=Mitochondrial oligopeptidase M; AltName: Full=Neurotensin endopeptidase; Flags: Precursor;
FUNCTION: Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A (By similarity).
CATALYTIC ACTIVITY: Preferential cleavage in neurotensin: 10- Pro-|-Tyr-11.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBCELLULAR LOCATION: Mitochondrion intermembrane space (By similarity). Cytoplasm (By similarity).
SIMILARITY: Belongs to the peptidase M3 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): NLN
CDC HuGE Published Literature: NLN

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.63 RPKM in Cells - Transformed fibroblasts
Total median expression: 89.08 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -96.10178-0.540 Picture PostScript Text
3' UTR -1796.056368-0.282 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024079 - MetalloPept_cat_dom
IPR024077 - Neurolysin/TOP_dom2
IPR024080 - Neurolysin/TOP_N
IPR001567 - Pept_M3A_M3B

Pfam Domains:
PF01432 - Peptidase family M3

SCOP Domains:
55486 - Metalloproteases ("zincins"), catalytic domain

ModBase Predicted Comparative 3D Structure on Q9BYT8
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 Protein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004222 metalloendopeptidase activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0016787 hydrolase activity
GO:0042277 peptide binding
GO:0046872 metal ion binding

Biological Process:
GO:0006111 regulation of gluconeogenesis
GO:0006508 proteolysis
GO:0006518 peptide metabolic process
GO:1902809 regulation of skeletal muscle fiber differentiation

Cellular Component:
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005758 mitochondrial intermembrane space
GO:0005886 plasma membrane

-  Descriptions from all associated GenBank mRNAs
  AK096416 - Homo sapiens cDNA FLJ39097 fis, clone NTONG2000977, highly similar to Neurolysin, mitochondrial precursor (EC
AK292803 - Homo sapiens cDNA FLJ76106 complete cds, highly similar to Homo sapiens neurolysin (metallopeptidase M3 family) (NLN), mRNA.
BC001644 - Homo sapiens neurolysin (metallopeptidase M3 family), mRNA (cDNA clone MGC:2361 IMAGE:2958628), complete cds.
BC004985 - Homo sapiens neurolysin (metallopeptidase M3 family), mRNA (cDNA clone MGC:3978 IMAGE:2958628), complete cds.
AJ300837 - Homo sapiens mRNA for neurolysin.
JD200336 - Sequence 181360 from Patent EP1572962.
JD318782 - Sequence 299806 from Patent EP1572962.
JD524647 - Sequence 505671 from Patent EP1572962.
AB463138 - Synthetic construct DNA, clone: pF1KA1226, Homo sapiens NLN gene for neurolysin, without stop codon, in Flexi system.
CU674806 - Synthetic construct Homo sapiens gateway clone IMAGE:100017425 5' read NLN mRNA.
KJ902956 - Synthetic construct Homo sapiens clone ccsbBroadEn_12350 NLN gene, encodes complete protein.
AB033052 - Homo sapiens KIAA1226 mRNA for KIAA1226 protein.
AK027602 - Homo sapiens cDNA FLJ14696 fis, clone NT2RP2005775, highly similar to NEUROLYSIN PRECURSOR (EC
AL834241 - Homo sapiens mRNA; cDNA DKFZp564F123 (from clone DKFZp564F123).
JD143152 - Sequence 124176 from Patent EP1572962.
JD362610 - Sequence 343634 from Patent EP1572962.
JD365139 - Sequence 346163 from Patent EP1572962.
JD549481 - Sequence 530505 from Patent EP1572962.
JD434549 - Sequence 415573 from Patent EP1572962.
JD312855 - Sequence 293879 from Patent EP1572962.
JD491627 - Sequence 472651 from Patent EP1572962.
JD432834 - Sequence 413858 from Patent EP1572962.
JD209571 - Sequence 190595 from Patent EP1572962.
JD128545 - Sequence 109569 from Patent EP1572962.
JD534741 - Sequence 515765 from Patent EP1572962.
JD366237 - Sequence 347261 from Patent EP1572962.
JD212425 - Sequence 193449 from Patent EP1572962.
JD286811 - Sequence 267835 from Patent EP1572962.
JD286813 - Sequence 267837 from Patent EP1572962.
JD286815 - Sequence 267839 from Patent EP1572962.
JD511602 - Sequence 492626 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04614 - Renin-angiotensin system

Reactome (by CSHL, EBI, and GO)

Protein Q9BYT8 (Reactome details) participates in the following event(s):

R-HSA-8940959 Neurolysin degrades neurotensin
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: AGTBP, KIAA1226, NEUL_HUMAN, NM_020726, NP_065777, Q9BYT8, Q9ULJ4
UCSC ID: uc003juf.3
RefSeq Accession: NM_020726
Protein: Q9BYT8 (aka NEUL_HUMAN)
CCDS: CCDS3989.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_020726.4
exon count: 13CDS single in 3' UTR: no RNA size: 8672
ORF size: 2115CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4330.00frame shift in genome: no % Coverage: 99.87
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.