Description: Homo sapiens RAD18 homolog (S. cerevisiae) (RAD18), mRNA. RefSeq Summary (NM_020165): The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Transcript (Including UTRs) Position: hg19 chr3:8,918,880-9,005,159 Size: 86,280 Total Exon Count: 13 Strand: - Coding Region Position: hg19 chr3:8,923,041-9,005,069 Size: 82,029 Coding Exon Count: 13
ID:RAD18_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase RAD18; EC=6.3.2.-; AltName: Full=Postreplication repair protein RAD18; Short=hHR18; Short=hRAD18; AltName: Full=RING finger protein 73; FUNCTION: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with UBE2A and UBE2B. Interacts with HLTF and SHPRH. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. SUBCELLULAR LOCATION: Nucleus (By similarity). SIMILARITY: Belongs to the RAD18 family. SIMILARITY: Contains 1 RING-type zinc finger. SIMILARITY: Contains 1 SAP domain. SIMILARITY: Contains 1 UBZ-type zinc finger. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rad18/";
Genetic Association Studies of Complex Diseases and Disorders
Genetic Association Database (archive): RAD18 CDC HuGE Published Literature: RAD18 Positive Disease Associations: smoking cessation Related Studies:
smoking cessation Uhl ,et al. Pharmacogenomics 2010, Genome-wide association for smoking cessation success: participants in the Patch in Practice trial of nicotine replacement , Pharmacogenomics 2010 11- 3 : 357-67.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9NS91
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.