Human Gene RAD18 (uc003brd.3)
  Description: Homo sapiens RAD18 homolog (S. cerevisiae) (RAD18), mRNA.
RefSeq Summary (NM_020165): The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Transcript (Including UTRs)
   Position: hg19 chr3:8,918,880-9,005,159 Size: 86,280 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chr3:8,923,041-9,005,069 Size: 82,029 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:8,918,880-9,005,159)mRNA (may differ from genome)Protein (495 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSCGAPEnsemblEntrez GeneExonPrimer
GeneCardsGeneNetworkH-INVHGNCHPRDLynx
MGIneXtProtOMIMPubMedReactomeTreefam
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: RAD18_HUMAN
DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase RAD18; EC=6.3.2.-; AltName: Full=Postreplication repair protein RAD18; Short=hHR18; Short=hRAD18; AltName: Full=RING finger protein 73;
FUNCTION: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with UBE2A and UBE2B. Interacts with HLTF and SHPRH. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the RAD18 family.
SIMILARITY: Contains 1 RING-type zinc finger.
SIMILARITY: Contains 1 SAP domain.
SIMILARITY: Contains 1 UBZ-type zinc finger.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rad18/";

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): RAD18
CDC HuGE Published Literature: RAD18
Positive Disease Associations: smoking cessation
Related Studies:
  1. smoking cessation
    Uhl ,et al. Pharmacogenomics 2010, Genome-wide association for smoking cessation success: participants in the Patch in Practice trial of nicotine replacement , Pharmacogenomics 2010 11- 3 : 357-67. [PubMed 20235792]

-  MalaCards Disease Associations
  MalaCards Gene Search: RAD18
Diseases sorted by gene-association score: fanconi anemia, complementation group a (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 6.52 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 82.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -31.8090-0.353 Picture PostScript Text
3' UTR -1208.044161-0.290 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003034 - SAP_DNA-bd
IPR006642 - Znf_Rad18_put
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD
IPR017907 - Znf_RING_CS

Pfam Domains:
PF00097 - Zinc finger, C3HC4 type (RING finger)
PF02037 - SAP domain
PF13445 - RING-type zinc-finger
PF13923 - Zinc finger, C3HC4 type (RING finger)

SCOP Domains:
57850 - RING/U-box

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2Y43 - X-ray MuPIT 2YBF - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NS91
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologGenome Browser
Gene Details    Gene Details
Gene Sorter    Gene Sorter
  Ensembl  SGD
  Protein Sequence  Protein Sequence
  Alignment  Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000403 Y-form DNA binding
GO:0003677 DNA binding
GO:0003684 damaged DNA binding
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0031593 polyubiquitin binding
GO:0031625 ubiquitin protein ligase binding
GO:0042802 identical protein binding
GO:0043142 single-stranded DNA-dependent ATPase activity
GO:0044877 macromolecular complex binding
GO:0046872 metal ion binding
GO:0061630 ubiquitin protein ligase activity

Biological Process:
GO:0006281 DNA repair
GO:0006301 postreplication repair
GO:0006513 protein monoubiquitination
GO:0006974 cellular response to DNA damage stimulus
GO:0009411 response to UV
GO:0016567 protein ubiquitination
GO:0042769 DNA damage response, detection of DNA damage
GO:0051865 protein autoubiquitination
GO:0051984 positive regulation of chromosome segregation
GO:0060548 negative regulation of cell death

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005657 replication fork
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005856 cytoskeleton
GO:0016604 nuclear body
GO:0035861 site of double-strand break
GO:0042405 nuclear inclusion body
GO:0097505 Rad6-Rad18 complex


-  Descriptions from all associated GenBank mRNAs
  AK123966 - Homo sapiens cDNA FLJ41972 fis, clone SKNMC2003987.
LF384164 - JP 2014500723-A/191667: Polycomb-Associated Non-Coding RNAs.
AF169796 - Homo sapiens zinc finger DNA binding protein (RAD18) mRNA, complete cds.
JD305811 - Sequence 286835 from Patent EP1572962.
JD360369 - Sequence 341393 from Patent EP1572962.
JD421810 - Sequence 402834 from Patent EP1572962.
AB035274 - Homo sapiens mRNA for postreplication repair protein hRAD18p, complete cds.
AK023075 - Homo sapiens cDNA FLJ13013 fis, clone NT2RP3000590, weakly similar to UVS-2 PROTEIN.
JD039602 - Sequence 20626 from Patent EP1572962.
JD419314 - Sequence 400338 from Patent EP1572962.
JD124353 - Sequence 105377 from Patent EP1572962.
JD369523 - Sequence 350547 from Patent EP1572962.
JD085304 - Sequence 66328 from Patent EP1572962.
JD349561 - Sequence 330585 from Patent EP1572962.
JD551608 - Sequence 532632 from Patent EP1572962.
LF364911 - JP 2014500723-A/172414: Polycomb-Associated Non-Coding RNAs.
JD115640 - Sequence 96664 from Patent EP1572962.
JD195159 - Sequence 176183 from Patent EP1572962.
JD378290 - Sequence 359314 from Patent EP1572962.
JD131376 - Sequence 112400 from Patent EP1572962.
JD517213 - Sequence 498237 from Patent EP1572962.
JD251622 - Sequence 232646 from Patent EP1572962.
BC001302 - Homo sapiens RAD18 homolog (S. cerevisiae), mRNA (cDNA clone MGC:5478 IMAGE:3451960), complete cds.
JD065659 - Sequence 46683 from Patent EP1572962.
JD267275 - Sequence 248299 from Patent EP1572962.
AY004333 - Homo sapiens RAD18 (hHR18) mRNA, complete cds.
AK222771 - Homo sapiens mRNA for postreplication repair protein hRAD18p variant, clone: HEP00690.
DQ891197 - Synthetic construct clone IMAGE:100003827; FLH170080.01X; RZPDo839B0798D RAD18 homolog (S. cerevisiae) (RAD18) gene, encodes complete protein.
DQ894380 - Synthetic construct Homo sapiens clone IMAGE:100008840; FLH170076.01L; RZPDo839B0797D RAD18 homolog (S. cerevisiae) (RAD18) gene, encodes complete protein.
AB528316 - Synthetic construct DNA, clone: pF1KE1039, Homo sapiens RAD18 gene for RAD18 homolog, without stop codon, in Flexi system.
LF364915 - JP 2014500723-A/172418: Polycomb-Associated Non-Coding RNAs.
LF364928 - JP 2014500723-A/172431: Polycomb-Associated Non-Coding RNAs.
LF364937 - JP 2014500723-A/172440: Polycomb-Associated Non-Coding RNAs.
LF364940 - JP 2014500723-A/172443: Polycomb-Associated Non-Coding RNAs.
LF364942 - JP 2014500723-A/172445: Polycomb-Associated Non-Coding RNAs.
JD458868 - Sequence 439892 from Patent EP1572962.
MA619741 - JP 2018138019-A/191667: Polycomb-Associated Non-Coding RNAs.
MA600488 - JP 2018138019-A/172414: Polycomb-Associated Non-Coding RNAs.
MA600492 - JP 2018138019-A/172418: Polycomb-Associated Non-Coding RNAs.
MA600505 - JP 2018138019-A/172431: Polycomb-Associated Non-Coding RNAs.
MA600514 - JP 2018138019-A/172440: Polycomb-Associated Non-Coding RNAs.
MA600517 - JP 2018138019-A/172443: Polycomb-Associated Non-Coding RNAs.
MA600519 - JP 2018138019-A/172445: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NS91 (Reactome details) participates in the following event(s):

R-HSA-8943007 SHPRH binds monoUb-K164-PCNA, RAD6:RAD18, UBE2V2:Ub:UBE2N
R-HSA-8943041 HLTF binds monoUb-K164-PCNA, RAD6:RAD18, UBE2V2:Ub:UBE2N
R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA
R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-8852135 Protein ubiquitination
R-HSA-73893 DNA Damage Bypass
R-HSA-597592 Post-translational protein modification
R-HSA-73894 DNA Repair
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: NM_020165, NP_064550, Q58F55, Q9NRT6, Q9NS91, RAD18_HUMAN, RNF73
UCSC ID: uc003brd.3
RefSeq Accession: NM_020165
Protein: Q9NS91 (aka RAD18_HUMAN or RA18_HUMAN)
CCDS: CCDS2571.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_020165.3
exon count: 13CDS single in 3' UTR: no RNA size: 5739
ORF size: 1488CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3109.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.