Human Gene STAM2 (uc002tyc.4) Description and Page Index
  Description: Homo sapiens signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2), mRNA.
RefSeq Summary (NM_005843): The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.33883.1, SRR1660807.86413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263904.5/ ENSP00000263904.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr2:152,973,315-153,032,506 Size: 59,192 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr2:152,977,088-153,032,156 Size: 55,069 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:152,973,315-153,032,506)mRNA (may differ from genome)Protein (525 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeStanford SOURCETreefam
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: STAM2_HUMAN
DESCRIPTION: RecName: Full=Signal transducing adapter molecule 2; Short=STAM-2; AltName: Full=Hrs-binding protein;
FUNCTION: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
SUBUNIT: Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct.
INTERACTION: Q13671:RIN1; NbExp=4; IntAct=EBI-373258, EBI-366017; O95630:STAMBP; NbExp=3; IntAct=EBI-373258, EBI-396676;
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Early endosome membrane; Peripheral membrane protein; Cytoplasmic side.
TISSUE SPECIFICITY: Ubiquitously expressed.
DOMAIN: The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
DOMAIN: The SH3 domain mediates the interaction with USP8.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
SIMILARITY: Belongs to the STAM family.
SIMILARITY: Contains 1 ITAM domain.
SIMILARITY: Contains 1 SH3 domain.
SIMILARITY: Contains 1 UIM (ubiquitin-interacting motif) repeat.
SIMILARITY: Contains 1 VHS domain.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.50 RPKM in Testis
Total median expression: 276.17 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -174.50350-0.499 Picture PostScript Text
3' UTR -930.403773-0.247 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008942 - ENTH_VHS
IPR000108 - p67phox
IPR001452 - SH3_domain
IPR003903 - Ubiquitin-int_motif
IPR002014 - VHS
IPR018205 - VHS_subgr

Pfam Domains:
PF00018 - SH3 domain
PF00790 - VHS domain
PF02809 - Ubiquitin interaction motif
PF07653 - Variant SH3 domain
PF14604 - Variant SH3 domain

SCOP Domains:
48464 - ENTH/VHS domain
50044 - SH3-domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1X2Q
- NMR MuPIT

1X5B
- NMR MuPIT

2L0T
- NMR MuPIT


ModBase Predicted Comparative 3D Structure on O75886
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006886 intracellular protein transport
GO:0015031 protein transport
GO:0016197 endosomal transport
GO:0016236 macroautophagy
GO:0036258 multivesicular body assembly
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0061024 membrane organization

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005768 endosome
GO:0005829 cytosol
GO:0016020 membrane
GO:0031901 early endosome membrane
GO:0033565 ESCRT-0 complex
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  JD158468 - Sequence 139492 from Patent EP1572962.
JD400769 - Sequence 381793 from Patent EP1572962.
AL133600 - Homo sapiens mRNA; cDNA DKFZp564C047 (from clone DKFZp564C047).
JD357181 - Sequence 338205 from Patent EP1572962.
JD235389 - Sequence 216413 from Patent EP1572962.
JD260015 - Sequence 241039 from Patent EP1572962.
JD361550 - Sequence 342574 from Patent EP1572962.
JD309764 - Sequence 290788 from Patent EP1572962.
JD162577 - Sequence 143601 from Patent EP1572962.
JD129920 - Sequence 110944 from Patent EP1572962.
JD231994 - Sequence 213018 from Patent EP1572962.
JD124303 - Sequence 105327 from Patent EP1572962.
JD067386 - Sequence 48410 from Patent EP1572962.
JD546660 - Sequence 527684 from Patent EP1572962.
JD381768 - Sequence 362792 from Patent EP1572962.
JD333866 - Sequence 314890 from Patent EP1572962.
JD513565 - Sequence 494589 from Patent EP1572962.
JD513564 - Sequence 494588 from Patent EP1572962.
AK292847 - Homo sapiens cDNA FLJ76170 complete cds, highly similar to Homo sapiens signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2), mRNA.
AF042273 - Homo sapiens signal transducing adaptor molecule 2A (STAM2) mRNA, complete cds.
AK023490 - Homo sapiens cDNA FLJ13428 fis, clone PLACE1002493, highly similar to Homo sapiens signal transducing adaptor molecule 2A (STAM2) mRNA.
BC028740 - Homo sapiens signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, mRNA (cDNA clone MGC:33781 IMAGE:5260998), complete cds.
JD047090 - Sequence 28114 from Patent EP1572962.
AK292265 - Homo sapiens cDNA FLJ78529 complete cds, highly similar to Homo sapiens signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, mRNA.
JD193287 - Sequence 174311 from Patent EP1572962.
DQ892427 - Synthetic construct clone IMAGE:100005057; FLH186238.01X; RZPDo839C0571D signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2) gene, encodes complete protein.
KJ901058 - Synthetic construct Homo sapiens clone ccsbBroadEn_10452 STAM2 gene, encodes complete protein.
DQ895641 - Synthetic construct Homo sapiens clone IMAGE:100010101; FLH186234.01L; RZPDo839C0561D signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2) gene, encodes complete protein.
AB463258 - Synthetic construct DNA, clone: pF1KB8674, Homo sapiens STAM2 gene for signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, without stop codon, in Flexi system.
AM393419 - Synthetic construct Homo sapiens clone IMAGE:100001885 for hypothetical protein (STAM2 gene).
AK310863 - Homo sapiens cDNA, FLJ17905.
AF042274 - Homo sapiens signal transducing adaptor molecule 2B (STAM2) mRNA, complete cds.
CU688564 - Synthetic construct Homo sapiens gateway clone IMAGE:100020287 5' read STAM2 mRNA.
BC022277 - Homo sapiens cDNA clone IMAGE:4693561, with apparent retained intron.
JD407011 - Sequence 388035 from Patent EP1572962.
JD470086 - Sequence 451110 from Patent EP1572962.
JD440054 - Sequence 421078 from Patent EP1572962.
JD191317 - Sequence 172341 from Patent EP1572962.
JD112062 - Sequence 93086 from Patent EP1572962.
JD193599 - Sequence 174623 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04144 - Endocytosis
hsa04630 - Jak-STAT signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein O75886 (Reactome details) participates in the following event(s):

R-HSA-6782628 USP8 deubiquitinates STAM2:HGS
R-HSA-183072 EGFR non-clathrin mediated endocytosis
R-HSA-8867044 EGFR binds EPS15, EPN1, EPS15L1
R-HSA-8875490 EPS15 and HGS bind ubiquitinated MET
R-HSA-8876262 EPS15 and HGS bind CBL-monoubiquitinated MET engaged with Listeria InlB
R-HSA-917730 Cargo Recognition And Sorting
R-HSA-182986 CBL-mediated ubiquitination of CIN85
R-HSA-182990 Sprouty sequesters CBL away from active EGFR
R-HSA-8867041 EGFR phosphorylates EPS15
R-HSA-8867047 PTPN3 dephosphorylates EPS15
R-HSA-8866279 Epsin family proteins bind ubiquitinated cargo
R-HSA-8867756 CLASP proteins and cargo are recruited to the nascent clathrin-coated pit
R-HSA-8868071 Clathrin recruits PIK3C2A
R-HSA-8868661 Dynamin-mediated GTP hydrolysis promotes vesicle scission
R-HSA-8868648 SYNJ hydrolyze PI(4,5)P2 to PI(4)P
R-HSA-8871194 RAB5 and GAPVD1 bind AP-2
R-HSA-8868658 HSPA8-mediated ATP hydrolysis promotes vesicle uncoating
R-HSA-8868659 Clathrin recruits auxilins to the clathrin-coated vesicle
R-HSA-8868660 Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle
R-HSA-8867754 F- and N- BAR domain proteins bind the clathrin-coated pit
R-HSA-8868230 SNX9 recruits components of the actin polymerizing machinery
R-HSA-8868072 Clathrin-associated PIK3C2A phosphorylates PI(4)P to PI(3,4)P2
R-HSA-8868236 BAR domain proteins recruit dynamin
R-HSA-8868651 Endophilins recruit synaptojanins to the clathrin-coated pit
R-HSA-5689880 Ub-specific processing proteases
R-HSA-5688426 Deubiquitination
R-HSA-182971 EGFR downregulation
R-HSA-6807004 Negative regulation of MET activity
R-HSA-8875360 InlB-mediated entry of Listeria monocytogenes into host cell
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)
R-HSA-597592 Post-translational protein modification
R-HSA-177929 Signaling by EGFR
R-HSA-6806834 Signaling by MET
R-HSA-8876384 Listeria monocytogenes entry into host cells
R-HSA-199991 Membrane Trafficking
R-HSA-392499 Metabolism of proteins
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-5663205 Infectious disease
R-HSA-5653656 Vesicle-mediated transport
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-162582 Signal Transduction
R-HSA-1643685 Disease
R-HSA-8856828 Clathrin-mediated endocytosis

-  Other Names for This Gene
  Alternate Gene Symbols: A8K8A0, D3DPA1, HBP, NM_005843, NP_005834, O75886, Q7LDQ0, Q9UF58, STAM2_HUMAN
UCSC ID: uc002tyc.4
RefSeq Accession: NM_005843
Protein: O75886 (aka STAM2_HUMAN)
CCDS: CCDS2196.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_005843.4
exon count: 14CDS single in 3' UTR: no RNA size: 5701
ORF size: 1578CDS single in intron: no Alignment % ID: 99.98
txCdsPredict score: 3109.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.