Human Gene STK10 (uc003mbo.1) Description and Page Index
  Description: Homo sapiens serine/threonine kinase 10 (STK10), mRNA.
RefSeq Summary (NM_005990): This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.134568.1, BC070077.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000176763.10/ ENSP00000176763.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr5:171,469,074-171,615,346 Size: 146,273 Total Exon Count: 19 Strand: -
Coding Region
   Position: hg19 chr5:171,471,886-171,615,046 Size: 143,161 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:171,469,074-171,615,346)mRNA (may differ from genome)Protein (968 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeStanford SOURCETreefam
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: STK10_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase 10; EC=2.7.11.1; AltName: Full=Lymphocyte-oriented kinase;
FUNCTION: Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib.
SUBUNIT: Homodimer; homodimerization is required for activation segment autophosphorylation.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.
PTM: Autophosphorylates following homodimerization, leading to activation of the protein.
DISEASE: Defects in STK10 may be a cause of testicular germ cell tumor (TGCT) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
MISCELLANEOUS: Inhibition by erlotinib, an orally administered EGFR tyrosine kinase inhibitor used for treatment, enhances STK10- dependent lymphocytic responses, possibly leading to the aggravation of skin inflammation observed upon treatment by erlotinib (PubMed:21606217).
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=BAG51143.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): STK10
CDC HuGE Published Literature: STK10
Positive Disease Associations: Blood Cells , Cholesterol
Related Studies:
  1. Blood Cells
    Qiong Yang et al. BMC medical genetics 2007, Genome-wide association and linkage analyses of hemostatic factors and hematological phenotypes in the Framingham Heart Study., BMC medical genetics. [PubMed 17903294]
    Using genome-wide association methodology, we have successfully identified a SNP in complete LD with a sequence variant previously shown to be strongly associated with factor VII, providing proof of principle for this approach. Further study of additional strongly associated SNPs and linked regions may identify novel variants that influence the inter-individual variability in hemostatic factors and hematological phenotypes.
  2. Cholesterol
    , , . [PubMed 0]

-  MalaCards Disease Associations
  MalaCards Gene Search: STK10
Diseases sorted by gene-association score: testicular germ cell tumor* (147), seminal vesicle tumor (11), cecal disease (8), anus disease (7), biliary dyskinesia (7), cholecystolithiasis (7), splenic infarction (7), male reproductive organ benign neoplasm (7), hypotrichosis 7 (6)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 46.21 RPKM in Whole Blood
Total median expression: 348.59 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -175.40300-0.585 Picture PostScript Text
3' UTR -1042.462812-0.371 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR022165 - PKK
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase
PF12474 - Polo kinase kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help

2J7T
- X-ray MuPIT

4AOT
- X-ray MuPIT

4BC6
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O94804
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGD  WormBase 
 Protein Sequence  Protein Sequence 
 Alignment  Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0007346 regulation of mitotic cell cycle
GO:0016310 phosphorylation
GO:0023014 signal transduction by protein phosphorylation
GO:0031098 stress-activated protein kinase signaling cascade
GO:0032147 activation of protein kinase activity
GO:0042981 regulation of apoptotic process
GO:0043312 neutrophil degranulation
GO:0046777 protein autophosphorylation
GO:0071593 lymphocyte aggregation
GO:2000401 regulation of lymphocyte migration

Cellular Component:
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0035579 specific granule membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AB015718 - Homo sapiens lok mRNA for protein kinase, complete cds.
BC070077 - Homo sapiens serine/threonine kinase 10, mRNA (cDNA clone MGC:87394 IMAGE:5267449), complete cds.
AK307943 - Homo sapiens cDNA, FLJ97891.
AK313350 - Homo sapiens cDNA, FLJ93874, Homo sapiens serine/threonine kinase 10 (STK10), mRNA.
AK092814 - Homo sapiens cDNA FLJ35495 fis, clone SMINT2008817.
JD220056 - Sequence 201080 from Patent EP1572962.
BC014413 - Homo sapiens serine/threonine kinase 10, mRNA (cDNA clone IMAGE:4559697).
AF119894 - Homo sapiens PRO2729 mRNA, complete cds.
AK022960 - Homo sapiens cDNA FLJ12898 fis, clone NT2RP2004239, highly similar to Serine/threonine-protein kinase 10 (EC 2.7.11.1).
AL133081 - Homo sapiens mRNA; cDNA DKFZp434A0827 (from clone DKFZp434A0827).
JD289537 - Sequence 270561 from Patent EP1572962.
JD253247 - Sequence 234271 from Patent EP1572962.
JD191586 - Sequence 172610 from Patent EP1572962.
JD252142 - Sequence 233166 from Patent EP1572962.
KJ901770 - Synthetic construct Homo sapiens clone ccsbBroadEn_11164 STK10 gene, encodes complete protein.
JD163271 - Sequence 144295 from Patent EP1572962.
JD466934 - Sequence 447958 from Patent EP1572962.
JD195082 - Sequence 176106 from Patent EP1572962.
JD165669 - Sequence 146693 from Patent EP1572962.
JD089166 - Sequence 70190 from Patent EP1572962.
JD333080 - Sequence 314104 from Patent EP1572962.
JD110886 - Sequence 91910 from Patent EP1572962.
JD109201 - Sequence 90225 from Patent EP1572962.
JD492715 - Sequence 473739 from Patent EP1572962.
JD151470 - Sequence 132494 from Patent EP1572962.
JD245343 - Sequence 226367 from Patent EP1572962.
JD391496 - Sequence 372520 from Patent EP1572962.
JD439705 - Sequence 420729 from Patent EP1572962.
JD089755 - Sequence 70779 from Patent EP1572962.
JD259746 - Sequence 240770 from Patent EP1572962.
JD408934 - Sequence 389958 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O94804 (Reactome details) participates in the following event(s):

R-HSA-6799350 Exocytosis of specific granule membrane proteins
R-HSA-6798695 Neutrophil degranulation
R-HSA-168249 Innate Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A6ND35, B2R8F5, B3KMY1, LOK, NM_005990, NP_005981, O94804, Q6NSK0, Q9UIW4, STK10_HUMAN
UCSC ID: uc003mbo.1
RefSeq Accession: NM_005990
Protein: O94804 (aka STK10_HUMAN or STKA_HUMAN)
CCDS: CCDS34290.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_005990.3
exon count: 19CDS single in 3' UTR: no RNA size: 6039
ORF size: 2907CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 5879.00frame shift in genome: no % Coverage: 99.67
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.