Human Gene TANK (uc002ubr.2)
  Description: Homo sapiens TRAF family member-associated NFKB activator (TANK), transcript variant 1, mRNA.
RefSeq Summary (NM_004180): The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010].
Transcript (Including UTRs)
   Position: hg19 chr2:161,993,466-162,092,683 Size: 99,218 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr2:162,036,174-162,092,029 Size: 55,856 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr2:161,993,466-162,092,683)mRNA (may differ from genome)Protein (425 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSCGAPEnsemblEntrez GeneExonPrimer

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=TRAF family member-associated NF-kappa-B activator; AltName: Full=TRAF-interacting protein; Short=I-TRAF;
FUNCTION: Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF- kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity.
SUBUNIT: Interacts with TBK1 (via TRAF-C domain). Interacts with TRAF1 (via TRAF-C domain). Interacts with TRAF2 (via TRAF-C domain); the interaction is disrupted by the phosphorylation of TANK by IKBKE. Interacts with TRAF3 (via TRAF-C domain); the interaction with TRAF3 is weaker than the interactions with TRAF1 and TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG.
INTERACTION: O96018:APBA3; NbExp=2; IntAct=EBI-356349, EBI-6115839; P53350:PLK1; NbExp=3; IntAct=EBI-356349, EBI-476768; Q9UHD2:TBK1; NbExp=3; IntAct=EBI-356349, EBI-356402; Q12933:TRAF2; NbExp=3; IntAct=EBI-356349, EBI-355744; Q13114:TRAF3; NbExp=3; IntAct=EBI-356349, EBI-357631;
PTM: Phosphorylated by IKBKE.
SIMILARITY: Contains 1 C2H2-type zinc finger.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): TANK
CDC HuGE Published Literature: TANK
Positive Disease Associations: Coronary Artery Disease
Related Studies:
  1. Coronary Artery Disease
    , , . [PubMed 0]

-  MalaCards Disease Associations
  MalaCards Gene Search: TANK
Diseases sorted by gene-association score: vaccinia (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.81 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 364.77 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -36.10158-0.228 Picture PostScript Text
3' UTR -131.92654-0.202 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024581 - TBD

Pfam Domains:
PF12845 - TBD domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1KZZ - X-ray MuPIT 1L0A - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on Q92844
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 Protein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0031625 ubiquitin protein ligase binding
GO:0035800 deubiquitinase activator activity
GO:0046872 metal ion binding
GO:0004843 thiol-dependent ubiquitin-specific protease activity

Biological Process:
GO:0006508 proteolysis
GO:0006974 cellular response to DNA damage stimulus
GO:0007165 signal transduction
GO:0007249 I-kappaB kinase/NF-kappaB signaling
GO:0016032 viral process
GO:0035666 TRIF-dependent toll-like receptor signaling pathway
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor
GO:0071479 cellular response to ionizing radiation
GO:1903003 positive regulation of protein deubiquitination
GO:2000158 positive regulation of ubiquitin-specific protease activity

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 macromolecular complex

-  Descriptions from all associated GenBank mRNAs
  U63830 - Human TRAF family member-associated NF-kB activator TANK mRNA, complete cds.
U59863 - Human TRAF-interacting protein I-TRAF mRNA, complete cds.
JD041875 - Sequence 22899 from Patent EP1572962.
AK313096 - Homo sapiens cDNA, FLJ93580, highly similar to Homo sapiens TRAF family member-associated NFKB activator (TANK), transcript variant 1, mRNA.
BC067779 - Homo sapiens TRAF family member-associated NFKB activator, mRNA (cDNA clone MGC:87157 IMAGE:5296558), complete cds.
JD114075 - Sequence 95099 from Patent EP1572962.
BC003388 - Homo sapiens TRAF family member-associated NFKB activator, mRNA (cDNA clone IMAGE:3446546), complete cds.
CU678452 - Synthetic construct Homo sapiens gateway clone IMAGE:100018509 5' read TANK mRNA.
BT009855 - Homo sapiens TRAF family member-associated NFKB activator mRNA, complete cds.
KJ892895 - Synthetic construct Homo sapiens clone ccsbBroadEn_02289 TANK gene, encodes complete protein.
CU688338 - Synthetic construct Homo sapiens gateway clone IMAGE:100022955 5' read TANK mRNA.
AB587422 - Synthetic construct DNA, clone: pF1KB6667, Homo sapiens TANK gene for TRAF family member-associated NFKB activator, without stop codon, in Flexi system.
KJ898137 - Synthetic construct Homo sapiens clone ccsbBroadEn_07531 TANK gene, encodes complete protein.
JD237777 - Sequence 218801 from Patent EP1572962.
JD202637 - Sequence 183661 from Patent EP1572962.
JD238603 - Sequence 219627 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04622 - RIG-I-like receptor signaling pathway

BioCarta from NCI Cancer Genome Anatomy Project
h_tnfr2Pathway - TNFR2 Signaling Pathway
h_stressPathway - TNF/Stress Related Signaling

Reactome (by CSHL, EBI, and GO)

Protein Q92844 (Reactome details) participates in the following event(s):

R-HSA-933537 Recruitment of TANK to TRAF6
R-HSA-5362486 TANK binds K63-poly-Ub-TRAF3:TICAM1:activated TLR4
R-HSA-9013985 TANK binds K63-poly-Ub-TRAF3:TICAM1:activated TLR3
R-HSA-933527 Recruitment of TBK1/IKK epsilon complex to TANK:TRAF6
R-HSA-936941 Recruitment of TBK1/IKK epsilon to K63-pUb-TANK:K63-pUb-TRAF3:TRIF:activated TLR4 followed by their phosphorylation
R-HSA-9013986 Recruitment of TBK1/IKK epsilon to K63-pUb-TANK:K63-pUb-TRAF3:TRIF:activated TLR3 followed by their phosphorylation
R-HSA-933538 Recruitment of IRF7 to TRAF6
R-HSA-933525 Phosphorylation and release of IRF7
R-HSA-166271 IRF3/IRF7 recruitment to p-TBK1/p-IKK epsilon bound to the activated TLR4
R-HSA-166245 Phosphorylation of IRF-3/IRF7 and their release from the activated TLR complex
R-HSA-9013979 IRF3/IRF7 recruitment to p-TBK1/p-IKK epsilon bound to the activated TLR3
R-HSA-9013978 Phosphorylation of IRF-3/IRF7 and their release from the activated TLR3 complex
R-HSA-933541 TRAF6 mediated IRF7 activation
R-HSA-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-HSA-9013973 TICAM1-dependent activation of IRF3/IRF7
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-937061 TRIF(TICAM1)-mediated TLR4 signaling
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-168249 Innate Immune System
R-HSA-166166 MyD88-independent TLR4 cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-168256 Immune System
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade

-  Other Names for This Gene
  Alternate Gene Symbols: D3DPB5, ITRAF, NM_004180, NP_001186064, Q7Z4J6, Q92844, Q92885, TANK_HUMAN, TRAF2
UCSC ID: uc002ubr.2
RefSeq Accession: NM_004180
Protein: Q92844 (aka TANK_HUMAN)
CCDS: CCDS2215.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_004180.2
exon count: 8CDS single in 3' UTR: no RNA size: 2089
ORF size: 1278CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2736.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.