Human Gene TSPAN33 (uc003vop.2)
  Description: Homo sapiens tetraspanin 33 (TSPAN33), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr7:128,784,712-128,809,534 Size: 24,823 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr7:128,784,821-128,807,715 Size: 22,895 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:128,784,712-128,809,534)mRNA (may differ from genome)Protein (283 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMalacards
MGIneXtProtOMIMPubMedTreefamUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TSN33_HUMAN
DESCRIPTION: RecName: Full=Tetraspanin-33; Short=Tspan-33; AltName: Full=Penumbra; Short=hPen; AltName: Full=Proerythroblast new membrane;
FUNCTION: Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors (By similarity).
SUBUNIT: Homodimer; disulfide-linked (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Predominantly expressed in erythroblasts.
SIMILARITY: Belongs to the tetraspanin (TM4SF) family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TSPAN33
Diseases sorted by gene-association score: intracranial vasospasm (3), basilar artery occlusion (2), subdural empyema (2), heart sarcoma (2), stroke, ischemic (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 109.90 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 542.62 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -64.20109-0.589 Picture PostScript Text
3' UTR -771.841819-0.424 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000301 - Tetraspanin
IPR018499 - Tetraspanin/Peripherin
IPR008952 - Tetraspanin_EC2

Pfam Domains:
PF00335 - Tetraspanin family

SCOP Domains:
48652 - Tetraspanin

ModBase Predicted Comparative 3D Structure on Q86UF1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
  Protein SequenceProtein Sequence  
  AlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0019899 enzyme binding

Biological Process:
GO:0007166 cell surface receptor signaling pathway
GO:0051604 protein maturation
GO:0072659 protein localization to plasma membrane

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0097197 tetraspanin-enriched microdomain


-  Descriptions from all associated GenBank mRNAs
  BC044244 - Homo sapiens tetraspanin 33, mRNA (cDNA clone MGC:50844 IMAGE:5759132), complete cds.
AY236849 - Homo sapiens penumbra (PEN) mRNA, complete cds.
JD478368 - Sequence 459392 from Patent EP1572962.
JD195824 - Sequence 176848 from Patent EP1572962.
CU692196 - Synthetic construct Homo sapiens gateway clone IMAGE:100021280 5' read TSPAN33 mRNA.
HQ448638 - Synthetic construct Homo sapiens clone IMAGE:100072077; CCSB011438_03 tetraspanin 33 (TSPAN33) gene, encodes complete protein.
KJ896078 - Synthetic construct Homo sapiens clone ccsbBroadEn_05472 TSPAN33 gene, encodes complete protein.
AF276891 - Homo sapiens penumbra mRNA, complete cds.
AK000208 - Homo sapiens cDNA FLJ20201 fis, clone COLF1210.
JD117808 - Sequence 98832 from Patent EP1572962.
JD467055 - Sequence 448079 from Patent EP1572962.
JD037329 - Sequence 18353 from Patent EP1572962.
JD382830 - Sequence 363854 from Patent EP1572962.
JD355953 - Sequence 336977 from Patent EP1572962.
JD354429 - Sequence 335453 from Patent EP1572962.
JD122533 - Sequence 103557 from Patent EP1572962.
JD287345 - Sequence 268369 from Patent EP1572962.
JD110313 - Sequence 91337 from Patent EP1572962.
JD496597 - Sequence 477621 from Patent EP1572962.
JD187200 - Sequence 168224 from Patent EP1572962.
JD117593 - Sequence 98617 from Patent EP1572962.
JD342828 - Sequence 323852 from Patent EP1572962.
JD396432 - Sequence 377456 from Patent EP1572962.
JD491271 - Sequence 472295 from Patent EP1572962.
JD305350 - Sequence 286374 from Patent EP1572962.
JD161411 - Sequence 142435 from Patent EP1572962.
JD486627 - Sequence 467651 from Patent EP1572962.
JD225761 - Sequence 206785 from Patent EP1572962.
JD554374 - Sequence 535398 from Patent EP1572962.
JD100832 - Sequence 81856 from Patent EP1572962.
JD485957 - Sequence 466981 from Patent EP1572962.
JD036348 - Sequence 17372 from Patent EP1572962.
JD277045 - Sequence 258069 from Patent EP1572962.
JD354260 - Sequence 335284 from Patent EP1572962.
JD254014 - Sequence 235038 from Patent EP1572962.
JD161977 - Sequence 143001 from Patent EP1572962.
JD501491 - Sequence 482515 from Patent EP1572962.
JD123275 - Sequence 104299 from Patent EP1572962.
JD463614 - Sequence 444638 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: NM_178562, NP_848657, PEN, Q86UF1, TSN33_HUMAN
UCSC ID: uc003vop.2
RefSeq Accession: NM_178562
Protein: Q86UF1 (aka TSN33_HUMAN)
CCDS: CCDS5810.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_178562.3
exon count: 8CDS single in 3' UTR: no RNA size: 2900
ORF size: 852CDS single in intron: no Alignment % ID: 99.96
txCdsPredict score: 1866.50frame shift in genome: no % Coverage: 95.86
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.