Description: Homo sapiens tissue specific transplantation antigen P35B (GFUS), mRNA. RefSeq Summary (NM_003313): Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr8:144,694,788-144,699,622 Size: 4,835 Total Exon Count: 11 Strand: - Coding Region Position: hg19 chr8:144,695,080-144,698,882 Size: 3,803 Coding Exon Count: 10
ID:FCL_HUMAN DESCRIPTION: RecName: Full=GDP-L-fucose synthase; EC=1.1.1.271; AltName: Full=GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; AltName: Full=Protein FX; AltName: Full=Red cell NADP(H)-binding protein; AltName: Full=Short-chain dehydrogenase/reductase family 4E member 1; FUNCTION: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. CATALYTIC ACTIVITY: GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-6- deoxy-alpha-D-mannose + NADPH. PATHWAY: Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2. SUBUNIT: Homodimer. SIMILARITY: Belongs to the fucose synthase family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13630
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0007159 leukocyte cell-cell adhesion GO:0008152 metabolic process GO:0009226 nucleotide-sugar biosynthetic process GO:0019673 GDP-mannose metabolic process GO:0022900 electron transport chain GO:0042351 'de novo' GDP-L-fucose biosynthetic process GO:0055114 oxidation-reduction process
AK096752 - Homo sapiens cDNA FLJ39433 fis, clone PROST2004142, highly similar to GDP-L-fucose synthetase (EC 1.1.1.271). FW339989 - Screening. HM005668 - Homo sapiens clone HTL-T-45a testis tissue sperm-binding protein Li 45a mRNA, complete cds. BC001941 - Homo sapiens tissue specific transplantation antigen P35B, mRNA (cDNA clone MGC:4302 IMAGE:2819332), complete cds. LP895607 - Sequence 471 from Patent EP3253886. U58766 - Homo sapiens FX mRNA, complete cds. BC093061 - Homo sapiens tissue specific transplantation antigen P35B, mRNA (cDNA clone MGC:111147 IMAGE:30370158), complete cds. JD516615 - Sequence 497639 from Patent EP1572962. JD191011 - Sequence 172035 from Patent EP1572962. JD140549 - Sequence 121573 from Patent EP1572962. JD217111 - Sequence 198135 from Patent EP1572962. JD323121 - Sequence 304145 from Patent EP1572962. JD339933 - Sequence 320957 from Patent EP1572962. JD156958 - Sequence 137982 from Patent EP1572962. JD470953 - Sequence 451977 from Patent EP1572962. JD462218 - Sequence 443242 from Patent EP1572962. JD056739 - Sequence 37763 from Patent EP1572962. AK313560 - Homo sapiens cDNA, FLJ94122, Homo sapiens tissue specific transplantation antigen P35B (TSTA3),mRNA. HQ447820 - Synthetic construct Homo sapiens clone IMAGE:100071163; CCSB003805_01 tissue specific transplantation antigen P35B (TSTA3) gene, encodes complete protein. KJ892326 - Synthetic construct Homo sapiens clone ccsbBroadEn_01720 TSTA3 gene, encodes complete protein. CU674946 - Synthetic construct Homo sapiens gateway clone IMAGE:100018908 5' read TSTA3 mRNA. AK303125 - Homo sapiens cDNA FLJ57685 complete cds, highly similar to GDP-L-fucose synthetase (EC 1.1.1.271).
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00051 - Fructose and mannose metabolism hsa00520 - Amino sugar and nucleotide sugar metabolism hsa01100 - Metabolic pathways
Reactome (by CSHL, EBI, and GO)
Protein Q13630 (Reactome details) participates in the following event(s):
R-HSA-6787642 TSTA3 dimer reduces GDP-KDGal to GDP-Fuc R-HSA-6787623 TSTA3 dimer epimerises GDP-DHDMan to GDP-KDGal R-HSA-6787639 GDP-fucose biosynthesis R-HSA-446219 Synthesis of substrates in N-glycan biosythesis R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein R-HSA-446203 Asparagine N-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins