Description: Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA. RefSeq Summary (NM_015958): This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr1:101,455,180-101,491,362 Size: 36,183 Total Exon Count: 7 Strand: - Coding Region Position: hg19 chr1:101,455,964-101,487,245 Size: 31,282 Coding Exon Count: 5
Perphenazine D E Adkins et al. Molecular psychiatry 2011, Genomewide pharmacogenomic study of metabolic side effects to antipsychotic drugs., Molecular psychiatry.
[PubMed 20195266]
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9H2P9-5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK125485 - Homo sapiens cDNA FLJ43496 fis, clone PEBLM2001465, highly similar to Probable diphthine synthase (EC 2.1.1.98). AK289351 - Homo sapiens cDNA FLJ76983 complete cds, highly similar to Homo sapiens DPH5 homolog (S. cerevisiae), mRNA. AF132964 - Homo sapiens CGI-30 protein mRNA, complete cds. JD551169 - Sequence 532193 from Patent EP1572962. JD486849 - Sequence 467873 from Patent EP1572962. BC009620 - Homo sapiens DPH5 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:3893940), complete cds. BC003660 - Homo sapiens DPH5 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:2988502), **** WARNING: chimeric clone ****. BC053857 - Homo sapiens DPH5 homolog (S. cerevisiae), mRNA (cDNA clone MGC:61450 IMAGE:5503038), complete cds. AF248965 - Homo sapiens NPD015 mRNA, complete cds. AK225469 - Homo sapiens mRNA for Probable diphthine synthase variant, clone: HSI07462. AK225707 - Homo sapiens mRNA for Probable diphthine synthase variant, clone: TMS02611. AF161492 - Homo sapiens HSPC143 mRNA, complete cds. BC034669 - Homo sapiens DPH5 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:5189198), with apparent retained intron. AK225951 - Homo sapiens mRNA for Probable diphthine synthase variant, clone: FCC115A08. JD402253 - Sequence 383277 from Patent EP1572962. AK298181 - Homo sapiens cDNA FLJ60749 complete cds, moderately similar to Homo sapiens DPH5 homolog (DPH5), mRNA. JD548784 - Sequence 529808 from Patent EP1572962. AF157319 - Homo sapiens AD-018 protein mRNA, complete cds. KJ902607 - Synthetic construct Homo sapiens clone ccsbBroadEn_12001 DPH5 gene, encodes complete protein. AB590970 - Synthetic construct DNA, clone: pFN21AE1778, Homo sapiens DPH5 gene for DPH5 homolog, without stop codon, in Flexi system. JD358386 - Sequence 339410 from Patent EP1572962. DL491572 - Novel nucleic acids. DL490173 - Novel nucleic acids.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9H2P9 (Reactome details) participates in the following event(s):
R-HSA-5358484 DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2 R-HSA-5358493 Synthesis of diphthamide-EEF2 R-HSA-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins