Human Gene ZFAND4 (uc001jcm.4)
  Description: Homo sapiens zinc finger, AN1-type domain 4 (ZFAND4), transcript variant 1, mRNA.
Transcript (Including UTRs)
   Position: hg19 chr10:46,111,039-46,168,251 Size: 57,213 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr10:46,111,884-46,159,173 Size: 47,290 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:46,111,039-46,168,251)mRNA (may differ from genome)Protein (727 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtPubMedTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZFAN4_HUMAN
DESCRIPTION: RecName: Full=AN1-type zinc finger protein 4; AltName: Full=AN1-type zinc finger and ubiquitin domain-containing protein-like 1;
SIMILARITY: Contains 1 AN1-type zinc finger.
SIMILARITY: Contains 1 ubiquitin-like domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.03 RPKM in Testis
Total median expression: 93.43 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -316.44671-0.472 Picture PostScript Text
3' UTR -178.54845-0.211 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000626 - Ubiquitin
IPR019956 - Ubiquitin_subgr
IPR019955 - Ubiquitin_supergroup
IPR000058 - Znf_AN1

Pfam Domains:
PF00240 - Ubiquitin family
PF01428 - AN1-like Zinc finger
PF14560 - Ubiquitin-like domain

SCOP Domains:
54236 - Ubiquitin-like

ModBase Predicted Comparative 3D Structure on Q86XD8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008270 zinc ion binding
GO:0046872 metal ion binding


-  Descriptions from all associated GenBank mRNAs
  BC045587 - Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA (cDNA clone MGC:57415 IMAGE:5269483), complete cds.
AF311324 - Homo sapiens ubiquitin-like fusion protein mRNA, complete cds.
BC036186 - Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA (cDNA clone IMAGE:5303535), **** WARNING: chimeric clone ****.
BC048967 - Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA (cDNA clone IMAGE:5314527), **** WARNING: chimeric clone ****.
AK090704 - Homo sapiens cDNA FLJ33385 fis, clone BRACE2006547, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis) (ANUBL1), mRNA.
AX746581 - Sequence 106 from Patent EP1308459.
BC048968 - Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA (cDNA clone MGC:57202 IMAGE:5272133), complete cds.
AK292469 - Homo sapiens cDNA FLJ78689 complete cds, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA.
AK292420 - Homo sapiens cDNA FLJ75137 complete cds, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA.
AK097504 - Homo sapiens cDNA FLJ40185 fis, clone TESTI2018565, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis) (ANUBL1), mRNA.
AK314395 - Homo sapiens cDNA, FLJ95171, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis) (ANUBL1), mRNA.
JF432339 - Synthetic construct Homo sapiens clone IMAGE:100073527 AN1, ubiquitin-like, homolog (Xenopus laevis) (ANUBL1) gene, encodes complete protein.
KJ903602 - Synthetic construct Homo sapiens clone ccsbBroadEn_12996 ZFAND4 gene, encodes complete protein.
CU690590 - Synthetic construct Homo sapiens gateway clone IMAGE:100021004 5' read ANUBL1 mRNA.
BC017591 - Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis), mRNA (cDNA clone IMAGE:4828824), with apparent retained intron.
JD196962 - Sequence 177986 from Patent EP1572962.
JD356943 - Sequence 337967 from Patent EP1572962.
JD053265 - Sequence 34289 from Patent EP1572962.
JD350111 - Sequence 331135 from Patent EP1572962.
JD506373 - Sequence 487397 from Patent EP1572962.
JD565001 - Sequence 546025 from Patent EP1572962.
JD048061 - Sequence 29085 from Patent EP1572962.
JD250749 - Sequence 231773 from Patent EP1572962.
AK097924 - Homo sapiens cDNA FLJ40605 fis, clone THYMU2011852, highly similar to Homo sapiens AN1, ubiquitin-like, homolog (Xenopus laevis) (ANUBL1), mRNA.
JD462682 - Sequence 443706 from Patent EP1572962.
JD383675 - Sequence 364699 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K8V4, ANUBL1, B2RAX2, NM_174890, NP_777550, Q5VVY5, Q86XD8, ZFAN4_HUMAN
UCSC ID: uc001jcm.4
RefSeq Accession: NM_174890
Protein: Q86XD8 (aka ZFAN4_HUMAN)
CCDS: CCDS7214.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_174890.2
exon count: 10CDS single in 3' UTR: no RNA size: 3708
ORF size: 2184CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4413.00frame shift in genome: no % Coverage: 99.78
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.