Human Gene KRT76 (uc001sax.3)
  Description: Homo sapiens keratin 76 (KRT76), mRNA.
RefSeq Summary (NM_015848): Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009].
Transcript (Including UTRs)
   Position: hg19 chr12:53,161,939-53,171,129 Size: 9,191 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr12:53,162,497-53,171,075 Size: 8,579 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:53,161,939-53,171,129)mRNA (may differ from genome)Protein (638 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: K22O_HUMAN
DESCRIPTION: RecName: Full=Keratin, type II cytoskeletal 2 oral; AltName: Full=Cytokeratin-2P; Short=CK-2P; Short=K2P; AltName: Full=Keratin-76; Short=K76; AltName: Full=Type-II keratin Kb9;
FUNCTION: Probably contributes to terminal cornification.
SUBUNIT: Heterotetramer of two type I and two type II keratins.
DEVELOPMENTAL STAGE: Synthesized during maturation of epidermal keratinocytes.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
SIMILARITY: Belongs to the intermediate filament family.
SEQUENCE CAUTION: Sequence=BAG61375.1; Type=Erroneous termination; Positions=267; Note=Translated as Glu;
WEB RESOURCE: Name=Human Intermediate Filament Mutation Database; URL="http://www.interfil.org";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.02 RPKM in Testis
Total median expression: 0.05 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -11.7054-0.217 Picture PostScript Text
3' UTR -179.64558-0.322 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016044 - F
IPR001664 - IF
IPR018039 - Intermediate_filament_CS
IPR003054 - Keratin_II

Pfam Domains:
PF00038 - Intermediate filament protein
PF16208 - Keratin type II head

ModBase Predicted Comparative 3D Structure on Q01546
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005198 structural molecule activity

Biological Process:
GO:0007010 cytoskeleton organization
GO:0008544 epidermis development
GO:0031424 keratinization
GO:0043473 pigmentation
GO:0048733 sebaceous gland development
GO:0070268 cornification

Cellular Component:
GO:0005634 nucleus
GO:0005829 cytosol
GO:0005882 intermediate filament
GO:0045095 keratin filament
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  M99063 - Homo sapiens cytokeratin 2 mRNA, complete cds.
JD160445 - Sequence 141469 from Patent EP1572962.
JD126356 - Sequence 107380 from Patent EP1572962.
JD377024 - Sequence 358048 from Patent EP1572962.
JD456185 - Sequence 437209 from Patent EP1572962.
AK299390 - Homo sapiens cDNA FLJ60838 complete cds, highly similar to Keratin, type II cytoskeletal 2 oral.
JD184422 - Sequence 165446 from Patent EP1572962.
JD176590 - Sequence 157614 from Patent EP1572962.
JD425172 - Sequence 406196 from Patent EP1572962.
JD565387 - Sequence 546411 from Patent EP1572962.
JD270846 - Sequence 251870 from Patent EP1572962.
JD053941 - Sequence 34965 from Patent EP1572962.
JD503561 - Sequence 484585 from Patent EP1572962.
JD417953 - Sequence 398977 from Patent EP1572962.
BC167839 - Synthetic construct Homo sapiens clone IMAGE:100068229, MGC:195846 keratin 76 (KRT76) mRNA, encodes complete protein.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q01546 (Reactome details) participates in the following event(s):

R-HSA-6805546 Keratin type I binds keratin type II
R-HSA-6805573 Keratin type I/type II heterodimers form tetramers
R-HSA-6806613 Keratin tetramers bind to form unit length filaments
R-HSA-6805567 Keratinization
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: B4DRR3, K22O_HUMAN, KRT2B, KRT2P, NM_015848, NP_056932, Q01546, Q7Z795
UCSC ID: uc001sax.3
RefSeq Accession: NM_015848
Protein: Q01546 (aka K22O_HUMAN)
CCDS: CCDS8838.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_015848.4
exon count: 9CDS single in 3' UTR: no RNA size: 2529
ORF size: 1917CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4022.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.