Description: Homo sapiens TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 2, mRNA. RefSeq Summary (NM_134324): HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr12:53,895,377-53,900,215 Size: 4,839 Total Exon Count: 9 Strand: + Coding Region Position: hg19 chr12:53,895,809-53,899,932 Size: 4,124 Coding Exon Count: 8
ID:TRBP2_HUMAN DESCRIPTION: RecName: Full=RISC-loading complex subunit TARBP2; AltName: Full=TAR RNA-binding protein 2; AltName: Full=Trans-activation-responsive RNA-binding protein; FUNCTION: Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre- miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. SUBUNIT: Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9, EIF6, MOV10 and PRKRA. Associates with the 60S ribosome. INTERACTION: Q9UPY3:DICER1; NbExp=8; IntAct=EBI-978581, EBI-395506; P19525:EIF2AK2; NbExp=2; IntAct=EBI-978581, EBI-640775; SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, perinuclear region. Nucleus. SIMILARITY: Contains 3 DRBM (double-stranded RNA-binding) domains.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q15633
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006417 regulation of translation GO:0006469 negative regulation of protein kinase activity GO:0007286 spermatid development GO:0007338 single fertilization GO:0010586 miRNA metabolic process GO:0030422 production of siRNA involved in RNA interference GO:0030423 targeting of mRNA for destruction involved in RNA interference GO:0031047 gene silencing by RNA GO:0031054 pre-miRNA processing GO:0035087 siRNA loading onto RISC involved in RNA interference GO:0035196 production of miRNAs involved in gene silencing by miRNA GO:0035264 multicellular organism growth GO:0035280 miRNA loading onto RISC involved in gene silencing by miRNA GO:0045070 positive regulation of viral genome replication GO:0045727 positive regulation of translation GO:0046782 regulation of viral transcription GO:0050689 negative regulation of defense response to virus by host GO:0090065 regulation of production of siRNA involved in RNA interference GO:1903798 regulation of production of miRNAs involved in gene silencing by miRNA
US Server
