Human Gene RNASE7 (uc001vzk.4)
  Description: Homo sapiens ribonuclease, RNase A family, 7 (RNASE7), mRNA.
RefSeq Summary (NM_032572): The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein has broad-spectrum antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014].
Transcript (Including UTRs)
   Position: hg19 chr14:21,510,385-21,512,392 Size: 2,008 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr14:21,511,152-21,511,622 Size: 471 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:21,510,385-21,512,392)mRNA (may differ from genome)Protein (156 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RNAS7_HUMAN
DESCRIPTION: RecName: Full=Ribonuclease 7; Short=RNase 7; EC=3.1.27.-; AltName: Full=Skin-derived antimicrobial protein 2; Short=SAP-2; Flags: Precursor;
FUNCTION: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart.
SIMILARITY: Belongs to the pancreatic ribonuclease family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.35 RPKM in Skin - Sun Exposed (Lower leg)
Total median expression: 20.63 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -84.27257-0.328 Picture PostScript Text
3' UTR -242.98770-0.316 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001427 - RNaseA
IPR023411 - RNaseA_AS
IPR023412 - RNaseA_domain

Pfam Domains:
PF00074 - Pancreatic ribonuclease

SCOP Domains:
54076 - RNase A-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2HKY - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9H1E1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001530 lipopolysaccharide binding
GO:0003676 nucleic acid binding
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0004540 ribonuclease activity
GO:0016787 hydrolase activity
GO:0042834 peptidoglycan binding

Biological Process:
GO:0019730 antimicrobial humoral response
GO:0019731 antibacterial humoral response
GO:0042742 defense response to bacterium
GO:0045087 innate immune response
GO:0050829 defense response to Gram-negative bacterium
GO:0050830 defense response to Gram-positive bacterium
GO:0050832 defense response to fungus
GO:0051673 membrane disruption in other organism
GO:0090305 nucleic acid phosphodiester bond hydrolysis
GO:0090501 RNA phosphodiester bond hydrolysis

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK292697 - Homo sapiens cDNA FLJ75277 complete cds, highly similar to Homo sapiens ribonuclease, RNase A family, 7 (RNASE7), mRNA.
AY170392 - Homo sapiens ribonuclease 7 mRNA, complete cds.
JD134500 - Sequence 115524 from Patent EP1572962.
JD387350 - Sequence 368374 from Patent EP1572962.
JD335142 - Sequence 316166 from Patent EP1572962.
AJ306609 - Homo sapiens mRNA for ribonuclease 7 (RNASE7 gene), transcript variant 1.
AJ306610 - Homo sapiens mRNA for ribonuclease 7 (RNASE7 gene), transcript variant 2.
AJ306608 - Homo sapiens mRNA for ribonuclease 7 (RNASE7 gene), primary transcript.
JD408381 - Sequence 389405 from Patent EP1572962.
AY359097 - Homo sapiens clone DNA105782 RNASE7 (UNQ2516) mRNA, complete cds.
BC112334 - Homo sapiens ribonuclease, RNase A family, 7, mRNA (cDNA clone MGC:133220 IMAGE:40031904), complete cds.
BC074960 - Homo sapiens ribonuclease, RNase A family, 7, mRNA (cDNA clone MGC:104056 IMAGE:30915504), complete cds.
KJ899819 - Synthetic construct Homo sapiens clone ccsbBroadEn_09213 RNASE7 gene, encodes complete protein.
AJ131212 - Homo sapiens mRNA for ribonuclease 7.
JD358410 - Sequence 339434 from Patent EP1572962.
JD199070 - Sequence 180094 from Patent EP1572962.
JD463594 - Sequence 444618 from Patent EP1572962.
JD282526 - Sequence 263550 from Patent EP1572962.
JD095865 - Sequence 76889 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H1E1 (Reactome details) participates in the following event(s):

R-HSA-6803063 RNASEs bind bacterial LPS, PGN
R-HSA-8948027 RNASEs bind bacterial phospholipids
R-HSA-6803157 Antimicrobial peptides
R-HSA-168249 Innate Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: NM_032572, NP_115961, P80927, P83685, Q546N3, Q9H1E1, RNAS7_HUMAN, UNQ2516/PRO6006
UCSC ID: uc001vzk.4
RefSeq Accession: NM_032572
Protein: Q9H1E1 (aka RNAS7_HUMAN or RNS7_HUMAN)
CCDS: CCDS41914.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_032572.3
exon count: 2CDS single in 3' UTR: no RNA size: 1511
ORF size: 471CDS single in intron: no Alignment % ID: 99.93
txCdsPredict score: 1071.00frame shift in genome: no % Coverage: 99.14
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 621# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.