Human Gene METTL22 (uc002cyz.3)
  Description: Homo sapiens methyltransferase like 22 (METTL22), mRNA.
RefSeq Summary (NM_024109): This gene encodes a member of the non-histone lysine methyltransferases. It interacts with its substrate, Kin17, which is involved in DNA repair and replication and mRNA processing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].
Transcript (Including UTRs)
   Position: hg19 chr16:8,715,527-8,740,079 Size: 24,553 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg19 chr16:8,719,523-8,740,000 Size: 20,478 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:8,715,527-8,740,079)mRNA (may differ from genome)Protein (404 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeTreefamUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MET22_HUMAN
DESCRIPTION: RecName: Full=Methyltransferase-like protein 22; EC=2.1.1.-;
FUNCTION: Probable methyltransferase (By similarity).
SIMILARITY: Belongs to the methyltransferase superfamily. METTL22 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.18 RPKM in Testis
Total median expression: 183.06 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -126.20276-0.457 Picture PostScript Text
3' UTR -14.6079-0.185 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019410 - Nicotinamide_N-MeTfrase-like

Pfam Domains:
PF10294 - Lysine methyltransferase

SCOP Domains:
53335 - S-adenosyl-L-methionine-dependent methyltransferases

ModBase Predicted Comparative 3D Structure on Q9BUU2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
   Protein Sequence  
   Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008276 protein methyltransferase activity
GO:0016279 protein-lysine N-methyltransferase activity
GO:0016740 transferase activity
GO:0031072 heat shock protein binding

Biological Process:
GO:0006479 protein methylation
GO:0018022 peptidyl-lysine methylation
GO:0032259 methylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  LF385094 - JP 2014500723-A/192597: Polycomb-Associated Non-Coding RNAs.
AK315383 - Homo sapiens cDNA, FLJ96430.
BC001908 - Homo sapiens chromosome 16 open reading frame 68, mRNA (cDNA clone MGC:2654 IMAGE:3536628), complete cds.
AK022495 - Homo sapiens cDNA FLJ12433 fis, clone NT2RM1000032.
JD408235 - Sequence 389259 from Patent EP1572962.
JD275803 - Sequence 256827 from Patent EP1572962.
JD453803 - Sequence 434827 from Patent EP1572962.
JD525870 - Sequence 506894 from Patent EP1572962.
JD470719 - Sequence 451743 from Patent EP1572962.
CU674928 - Synthetic construct Homo sapiens gateway clone IMAGE:100017587 5' read C16orf68 mRNA.
KJ903150 - Synthetic construct Homo sapiens clone ccsbBroadEn_12544 METTL22 gene, encodes complete protein.
JD020323 - Sequence 1347 from Patent EP1572962.
AY203956 - Homo sapiens LP8272 mRNA, complete cds.
BC103725 - Homo sapiens chromosome 16 open reading frame 68, mRNA (cDNA clone IMAGE:5784399), with apparent retained intron.
AK000114 - Homo sapiens cDNA FLJ20107 fis, clone COL04839.
JD235399 - Sequence 216423 from Patent EP1572962.
JD460504 - Sequence 441528 from Patent EP1572962.
JD097387 - Sequence 78411 from Patent EP1572962.
JD391599 - Sequence 372623 from Patent EP1572962.
JD069490 - Sequence 50514 from Patent EP1572962.
JD411737 - Sequence 392761 from Patent EP1572962.
JD187522 - Sequence 168546 from Patent EP1572962.
JD496685 - Sequence 477709 from Patent EP1572962.
AK092846 - Homo sapiens cDNA FLJ35527 fis, clone SPLEN2001781.
AX747763 - Sequence 1288 from Patent EP1308459.
MA620671 - JP 2018138019-A/192597: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BUU2 (Reactome details) participates in the following event(s):

R-HSA-8932275 METTL22 transfers 3xCH3 from 3xAdoMet to KIN
R-HSA-8876725 Protein methylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B2RD29, C16orf68, D3DUF2, LP8272, MET22_HUMAN, NM_024109, NP_077014, Q6XYB4, Q9BUU2, Q9HA03
UCSC ID: uc002cyz.3
RefSeq Accession: NM_024109
Protein: Q9BUU2 (aka MET22_HUMAN)
CCDS: CCDS10533.2

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_024109.2
exon count: 11CDS single in 3' UTR: no RNA size: 1570
ORF size: 1215CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2529.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.