Human Gene DPEP2 (uc002eve.4)
  Description: Homo sapiens dipeptidase 2 (DPEP2), mRNA.
RefSeq Summary (NM_022355): DPEP2 belongs to the membrane-bound dipeptidase (EC 3.4.13.19) family. These enzymes hydrolyze a variety of dipeptides, including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (Habib et al., 2003 [PubMed 12738806]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg19 chr16:68,021,293-68,033,421 Size: 12,129 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr16:68,021,409-68,027,115 Size: 5,707 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:68,021,293-68,033,421)mRNA (may differ from genome)Protein (486 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DPEP2_HUMAN
DESCRIPTION: RecName: Full=Dipeptidase 2; EC=3.4.13.19; Flags: Precursor;
FUNCTION: Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4) (By similarity).
CATALYTIC ACTIVITY: Hydrolysis of dipeptides.
COFACTOR: Zinc (By similarity).
ENZYME REGULATION: Inhibited by L-penicillamine (By similarity).
SUBUNIT: Homodimer; disulfide-linked (By similarity).
SUBCELLULAR LOCATION: Membrane; Lipid-anchor, GPI-anchor (By similarity).
SIMILARITY: Belongs to the peptidase M19 family.
SEQUENCE CAUTION: Sequence=AAQ88819.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): DPEP2
CDC HuGE Published Literature: DPEP2
Positive Disease Associations: Cholesterol, HDL , Lipoproteins, HDL
Related Studies:
  1. Cholesterol, HDL
    Cristen J Willer et al. Nature genetics 2008, Newly identified loci that influence lipid concentrations and risk of coronary artery disease., Nature genetics. [PubMed 18193043]
  2. Lipoproteins, HDL
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.23 RPKM in Whole Blood
Total median expression: 59.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -62.90189-0.333 Picture PostScript Text
3' UTR -19.80116-0.171 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000180 - Pept_M19_AS
IPR008257 - Peptidase_M19

Pfam Domains:
PF01244 - Membrane dipeptidase (Peptidase family M19)

SCOP Domains:
51556 - Metallo-dependent hydrolases

ModBase Predicted Comparative 3D Structure on Q9H4A9
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsemblFlyBase  
 Protein SequenceProtein SequenceProtein Sequence  
 AlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008233 peptidase activity
GO:0008235 metalloexopeptidase activity
GO:0008237 metallopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0016787 hydrolase activity
GO:0016805 dipeptidase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis

Cellular Component:
GO:0016020 membrane
GO:0031225 anchored component of membrane


-  Descriptions from all associated GenBank mRNAs
  AJ295149 - Homo sapiens mRNA for putative dipeptidase.
AY358454 - Homo sapiens clone DNA35595 PGTY284 (UNQ284) mRNA, complete cds.
AK092884 - Homo sapiens cDNA FLJ35565 fis, clone SPLEN2005450, highly similar to Dipeptidase 2 precursor (EC 3.4.13.19).
AX747789 - Sequence 1314 from Patent EP1308459.
BC024021 - Homo sapiens dipeptidase 2, mRNA (cDNA clone MGC:27249 IMAGE:4616323), complete cds.
AK315090 - Homo sapiens cDNA, FLJ96047, Homo sapiens dipeptidase 2 (DPEP2), mRNA.
KJ903063 - Synthetic construct Homo sapiens clone ccsbBroadEn_12457 DPEP2 gene, encodes complete protein.
AB590838 - Synthetic construct DNA, clone: pFN21AE1940, Homo sapiens DPEP2 gene for dipeptidase 2, without stop codon, in Flexi system.
CU692848 - Synthetic construct Homo sapiens gateway clone IMAGE:100022303 5' read DPEP2 mRNA.
AK298003 - Homo sapiens cDNA FLJ55113 complete cds, moderately similar to Dipeptidase 2 precursor (EC 3.4.13.19).
JD193941 - Sequence 174965 from Patent EP1572962.
JD392849 - Sequence 373873 from Patent EP1572962.
JD115923 - Sequence 96947 from Patent EP1572962.
JD265385 - Sequence 246409 from Patent EP1572962.
JD419113 - Sequence 400137 from Patent EP1572962.
JD259454 - Sequence 240478 from Patent EP1572962.
JD156049 - Sequence 137073 from Patent EP1572962.
JD367460 - Sequence 348484 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H4A9 (Reactome details) participates in the following event(s):

R-HSA-266012 LTD4 is converted to LTE4 by DPEP1/2
R-HSA-5433067 DPEPs hydrolyse glycine from AFXBO-CG, AFNBO-CG
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-211859 Biological oxidations
R-HSA-8978868 Fatty acid metabolism
R-HSA-1430728 Metabolism
R-HSA-556833 Metabolism of lipids

-  Other Names for This Gene
  Alternate Gene Symbols: B2RCF8, DPEP2_HUMAN, NM_022355, NP_071750, Q6UX92, Q8TC95, Q9H4A9, uc002eve.3, UNQ284/PRO323
UCSC ID: uc002eve.4
RefSeq Accession: NM_022355
Protein: Q9H4A9 (aka DPEP2_HUMAN)
CCDS: CCDS10857.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_022355.3
exon count: 11CDS single in 3' UTR: no RNA size: 1781
ORF size: 1461CDS single in intron: no Alignment % ID: 99.94
txCdsPredict score: 3071.00frame shift in genome: no % Coverage: 99.16
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.