Human Gene RASD1 (uc002gri.3)
  Description: Homo sapiens RAS, dexamethasone-induced 1 (RASD1), transcript variant 1, mRNA.
RefSeq Summary (NM_016084): This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65, which interacts with the Alzheimer's disease amyloid precursor protein. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2011].
Transcript (Including UTRs)
   Position: hg19 chr17:17,397,753-17,399,709 Size: 1,957 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr17:17,398,439-17,399,495 Size: 1,057 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:17,397,753-17,399,709)mRNA (may differ from genome)Protein (281 aa)
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MGIneXtProtOMIMPubMedTreefamUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RASD1_HUMAN
DESCRIPTION: RecName: Full=Dexamethasone-induced Ras-related protein 1; AltName: Full=Activator of G-protein signaling 1; Flags: Precursor;
FUNCTION: Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65 (By similarity).
SUBUNIT: Forms a ternary complex with CAPON and NOS1. Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts with APBB1/FE65 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cytoplasm, perinuclear region (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues.
INDUCTION: By dexamethasone.
PTM: S-nitrosylation stimulates guanine-nucleotide exchange activity.
SIMILARITY: Belongs to the small GTPase superfamily. RasD family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: RASD1
Diseases sorted by gene-association score: multiple myeloma (3), prostate leiomyosarcoma (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 204.13 RPKM in Pituitary
Total median expression: 1739.66 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -67.80214-0.317 Picture PostScript Text
3' UTR -259.13686-0.378 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005225 - Small_GTP-bd_dom
IPR001806 - Small_GTPase
IPR020849 - Small_GTPase_Ras

Pfam Domains:
PF00025 - ADP-ribosylation factor family
PF00071 - Ras family
PF08477 - Ras of Complex, Roc, domain of DAPkinase

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on Q9Y272
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsemblFlyBaseWormBase 
 Protein SequenceProtein SequenceProtein SequenceProtein Sequence 
 AlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007263 nitric oxide mediated signal transduction
GO:0045892 negative regulation of transcription, DNA-templated

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016529 sarcoplasmic reticulum
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  LF212391 - JP 2014500723-A/19894: Polycomb-Associated Non-Coding RNAs.
LF209090 - JP 2014500723-A/16593: Polycomb-Associated Non-Coding RNAs.
AF177335 - Homo sapiens clone SP1942 unknown mRNA.
AF069506 - Homo sapiens activator of G protein signaling (AGS1) mRNA, complete cds.
BC042688 - Homo sapiens RAS, dexamethasone-induced 1, mRNA (cDNA clone IMAGE:5192139).
BC018041 - Homo sapiens RAS, dexamethasone-induced 1, mRNA (cDNA clone MGC:26290 IMAGE:4797482), complete cds.
LF326039 - JP 2014500723-A/133542: Polycomb-Associated Non-Coding RNAs.
JD038787 - Sequence 19811 from Patent EP1572962.
JD317516 - Sequence 298540 from Patent EP1572962.
AK294073 - Homo sapiens cDNA FLJ51813 complete cds, moderately similar to Dexamethasone-induced Ras-related protein 1.
LF326040 - JP 2014500723-A/133543: Polycomb-Associated Non-Coding RNAs.
AF153192 - Homo sapiens ras-related protein mRNA, complete cds.
JD172795 - Sequence 153819 from Patent EP1572962.
JD468948 - Sequence 449972 from Patent EP1572962.
JD203667 - Sequence 184691 from Patent EP1572962.
JD127671 - Sequence 108695 from Patent EP1572962.
JD141490 - Sequence 122514 from Patent EP1572962.
AF172846 - Homo sapiens ras-related protein (DEXRAS1) mRNA, complete cds.
JD538418 - Sequence 519442 from Patent EP1572962.
AF498923 - Homo sapiens activator of G protein signaling (RASD1) mRNA, complete cds.
AK312796 - Homo sapiens cDNA, FLJ93215, Homo sapiens RAS, dexamethasone-induced 1 (RASD1), mRNA.
KJ893963 - Synthetic construct Homo sapiens clone ccsbBroadEn_03357 RASD1 gene, encodes complete protein.
AM393716 - Synthetic construct Homo sapiens clone IMAGE:100002907 for hypothetical protein (RASD1 gene).
AB528221 - Synthetic construct DNA, clone: pF1KB5186, Homo sapiens RASD1 gene for RAS, dexamethasone-induced 1, without stop codon, in Flexi system.
AM392981 - Synthetic construct Homo sapiens clone IMAGE:100002638 for hypothetical protein (RASD1 gene).
AM393158 - Synthetic construct Homo sapiens clone IMAGE:100002639 for hypothetical protein (RASD1 gene).
LF213221 - JP 2014500723-A/20724: Polycomb-Associated Non-Coding RNAs.
CU676146 - Synthetic construct Homo sapiens gateway clone IMAGE:100018712 5' read RASD1 mRNA.
LF326041 - JP 2014500723-A/133544: Polycomb-Associated Non-Coding RNAs.
LF326042 - JP 2014500723-A/133545: Polycomb-Associated Non-Coding RNAs.
JD335610 - Sequence 316634 from Patent EP1572962.
MA561616 - JP 2018138019-A/133542: Polycomb-Associated Non-Coding RNAs.
MA561617 - JP 2018138019-A/133543: Polycomb-Associated Non-Coding RNAs.
MA561618 - JP 2018138019-A/133544: Polycomb-Associated Non-Coding RNAs.
MA561619 - JP 2018138019-A/133545: Polycomb-Associated Non-Coding RNAs.
MA447968 - JP 2018138019-A/19894: Polycomb-Associated Non-Coding RNAs.
MA444667 - JP 2018138019-A/16593: Polycomb-Associated Non-Coding RNAs.
MA448798 - JP 2018138019-A/20724: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: AGS1, B2R709, DEXRAS1, NM_016084, NP_057168, Q9NYB4, Q9Y272, RASD1_HUMAN
UCSC ID: uc002gri.3
RefSeq Accession: NM_016084
Protein: Q9Y272 (aka RASD1_HUMAN or RASD_HUMAN)
CCDS: CCDS11185.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_016084.4
exon count: 2CDS single in 3' UTR: no RNA size: 1814
ORF size: 846CDS single in intron: no Alignment % ID: 99.94
txCdsPredict score: 1848.50frame shift in genome: no % Coverage: 96.25
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.