Description: Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA. RefSeq Summary (NM_001042573): This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Transcript (Including UTRs) Position: hg19 chr17:77,071,019-77,084,685 Size: 13,667 Total Exon Count: 14 Strand: + Coding Region Position: hg19 chr17:77,071,027-77,082,431 Size: 11,405 Coding Exon Count: 14
ID:ENASE_HUMAN DESCRIPTION: RecName: Full=Cytosolic endo-beta-N-acetylglucosaminidase; Short=ENGase; EC=3.2.1.96; FUNCTION: Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol. CATALYTIC ACTIVITY: Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). TISSUE SPECIFICITY: Widely expressed. Expressed at higher level in thymus and spleen. SIMILARITY: Belongs to the glycosyl hydrolase 85 family. SIMILARITY: Contains 1 BRCT domain. SEQUENCE CAUTION: Sequence=AAH24213.2; Type=Frameshift; Positions=694;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF03644 - Glycosyl hydrolase family 85
ModBase Predicted Comparative 3D Structure on Q8NFI3
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK025518 - Homo sapiens cDNA: FLJ21865 fis, clone HEP02351. AK301115 - Homo sapiens cDNA FLJ57613 complete cds, highly similar to Homo sapiens endo-beta-N-acetylglucosaminidase, mRNA. BC156537 - Synthetic construct Homo sapiens clone IMAGE:100063240, MGC:190720 endo-beta-N-acetylglucosaminidase (FLJ21865) mRNA, encodes complete protein. AF512564 - Homo sapiens endo-beta-N-acetylglucosaminidase mRNA, complete cds. AL110283 - Homo sapiens mRNA; cDNA DKFZp434P174 (from clone DKFZp434P174). BC024213 - Homo sapiens endo-beta-N-acetylglucosaminidase, mRNA (cDNA clone IMAGE:3543906), partial cds. BC042158 - Homo sapiens, clone IMAGE:4303285, mRNA. DQ576470 - Homo sapiens piRNA piR-44582, complete sequence. JD080396 - Sequence 61420 from Patent EP1572962. JD162676 - Sequence 143700 from Patent EP1572962. JD515731 - Sequence 496755 from Patent EP1572962. JD389323 - Sequence 370347 from Patent EP1572962. JD153557 - Sequence 134581 from Patent EP1572962. JD500472 - Sequence 481496 from Patent EP1572962. JD115112 - Sequence 96136 from Patent EP1572962. JD318215 - Sequence 299239 from Patent EP1572962. JD547521 - Sequence 528545 from Patent EP1572962. JD164775 - Sequence 145799 from Patent EP1572962. JD075886 - Sequence 56910 from Patent EP1572962. JD187622 - Sequence 168646 from Patent EP1572962. JD135048 - Sequence 116072 from Patent EP1572962. JD194367 - Sequence 175391 from Patent EP1572962. JD086554 - Sequence 67578 from Patent EP1572962. JD475416 - Sequence 456440 from Patent EP1572962. JD375843 - Sequence 356867 from Patent EP1572962. JD260332 - Sequence 241356 from Patent EP1572962. JD154731 - Sequence 135755 from Patent EP1572962. JD377934 - Sequence 358958 from Patent EP1572962. JD277536 - Sequence 258560 from Patent EP1572962. JD482441 - Sequence 463465 from Patent EP1572962. JD235985 - Sequence 217009 from Patent EP1572962. JD380884 - Sequence 361908 from Patent EP1572962. JD373283 - Sequence 354307 from Patent EP1572962. JD472218 - Sequence 453242 from Patent EP1572962. JD519532 - Sequence 500556 from Patent EP1572962. JD272285 - Sequence 253309 from Patent EP1572962. JD483902 - Sequence 464926 from Patent EP1572962. JD174787 - Sequence 155811 from Patent EP1572962. JD061077 - Sequence 42101 from Patent EP1572962. JD161444 - Sequence 142468 from Patent EP1572962. JD272551 - Sequence 253575 from Patent EP1572962. JD174786 - Sequence 155810 from Patent EP1572962. JD161443 - Sequence 142467 from Patent EP1572962. JD216024 - Sequence 197048 from Patent EP1572962. JD378851 - Sequence 359875 from Patent EP1572962. JD559847 - Sequence 540871 from Patent EP1572962. JD047992 - Sequence 29016 from Patent EP1572962. JD395161 - Sequence 376185 from Patent EP1572962. JD433839 - Sequence 414863 from Patent EP1572962. JD390747 - Sequence 371771 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00511 - Other glycan degradation
Reactome (by CSHL, EBI, and GO)
Protein Q8NFI3 (Reactome details) participates in the following event(s):
R-HSA-8853379 ENGASE hydrolyses unfolded protein:(GlcNAc)2 (Man(9-5) R-HSA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle R-HSA-446203 Asparagine N-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins