Human Gene ZNF77 (uc002lws.4)
  Description: Homo sapiens zinc finger protein 77 (ZNF77), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr19:2,933,216-2,944,969 Size: 11,754 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr19:2,933,487-2,944,838 Size: 11,352 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:2,933,216-2,944,969)mRNA (may differ from genome)Protein (545 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZNF77_HUMAN
DESCRIPTION: RecName: Full=Zinc finger protein 77; AltName: Full=ZNFpT1;
FUNCTION: May be involved in transcriptional regulation.
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 12 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.
SEQUENCE CAUTION: Sequence=CAA46337.1; Type=Frameshift; Positions=98, 317;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ZNF77
Diseases sorted by gene-association score: tuberculous epididymitis (11), bladder diverticulum (11), bone squamous cell carcinoma (9), acute interstitial pneumonia (7), non-invasive bladder papillary urothelial neoplasm (7), cd40 ligand deficiency (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.58 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 84.44 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -62.90131-0.480 Picture PostScript Text
3' UTR -60.40271-0.223 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF00096 - Zinc finger, C2H2 type
PF01352 - KRAB box
PF13894 - C2H2-type zinc finger
PF13912 - C2H2-type zinc finger

SCOP Domains:
48695 - Multiheme cytochromes
109640 - KRAB domain (Kruppel-associated box, Pfam 01352)
57667 - C2H2 and C2HC zinc fingers

ModBase Predicted Comparative 3D Structure on Q15935
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003674 molecular_function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0008150 biological_process

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  BC043354 - Homo sapiens zinc finger protein 77, mRNA (cDNA clone MGC:49956 IMAGE:6014754), complete cds.
BC062561 - Homo sapiens zinc finger protein 77, mRNA (cDNA clone MGC:71324 IMAGE:6644579), complete cds.
AL365370 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 2107395.
AK290979 - Homo sapiens cDNA FLJ76041 complete cds, highly similar to Homo sapiens zinc finger protein 77 (pT1) (ZNF77), mRNA.
JD280360 - Sequence 261384 from Patent EP1572962.
HQ447596 - Synthetic construct Homo sapiens clone IMAGE:100070939; CCSB014794_03 zinc finger protein 77 (ZNF77) gene, encodes complete protein.
KJ894467 - Synthetic construct Homo sapiens clone ccsbBroadEn_03861 ZNF77 gene, encodes complete protein.
AB464415 - Synthetic construct DNA, clone: pF1KB7849, Homo sapiens ZNF77 gene for zinc finger protein 77, without stop codon, in Flexi system.
CU687558 - Synthetic construct Homo sapiens gateway clone IMAGE:100023065 5' read ZNF77 mRNA.
JD316290 - Sequence 297314 from Patent EP1572962.
X65230 - H.sapiens mRNA for Zinc-finger protein (ZNFpT1).
JD400514 - Sequence 381538 from Patent EP1572962.
JD410518 - Sequence 391542 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15935 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: NM_021217, NP_067040, Q15935, Q86XJ3, Q9NPP0, ZNF77_HUMAN
UCSC ID: uc002lws.4
RefSeq Accession: NM_021217
Protein: Q15935 (aka ZNF77_HUMAN or ZN77_HUMAN)
CCDS: CCDS12099.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_021217.2
exon count: 4CDS single in 3' UTR: no RNA size: 2057
ORF size: 1638CDS single in intron: no Alignment % ID: 99.90
txCdsPredict score: 3473.00frame shift in genome: no % Coverage: 99.17
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.