Human Gene TLE2 (uc002lww.3)
  Description: Homo sapiens transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1, mRNA.
Transcript (Including UTRs)
   Position: hg19 chr19:2,997,636-3,029,165 Size: 31,530 Total Exon Count: 20 Strand: -
Coding Region
   Position: hg19 chr19:2,997,846-3,028,902 Size: 31,057 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:2,997,636-3,029,165)mRNA (may differ from genome)Protein (743 aa)
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OMIMPubMedReactomeTreefamUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TLE2_HUMAN
DESCRIPTION: RecName: Full=Transducin-like enhancer protein 2; AltName: Full=Enhancer of split groucho-like protein 2; Short=ESG2;
FUNCTION: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).
SUBUNIT: Homooligomer and heterooligomer with other family members. Binds LEF1, TCF7, TCF7L1, TCF7L2, UTY, HES1 and HES5.
INTERACTION: Q16531:DDB1; NbExp=2; IntAct=EBI-1176061, EBI-350322;
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues examined, mostly in heart, brain, and muscle.
PTM: Ubiquitinated by XIAP/BIRC4.
SIMILARITY: Belongs to the WD repeat Groucho/TLE family.
SIMILARITY: Contains 6 WD repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): TLE2
CDC HuGE Published Literature: TLE2
Positive Disease Associations: Cholesterol , Cholesterol, LDL
Related Studies:
  1. Cholesterol
    , , . [PubMed 0]
  2. Cholesterol, LDL
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 198.06 RPKM in Brain - Cerebellum
Total median expression: 1331.22 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -111.57263-0.424 Picture PostScript Text
3' UTR -68.10210-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005617 - Groucho/TLE_N
IPR009146 - Groucho_enhance
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat
PF03920 - Groucho/TLE N-terminal Q-rich domain

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Prolyl oligopeptidase, N-terminal domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - Dipeptidyl peptidase IV/CD26, N-terminal domain

ModBase Predicted Comparative 3D Structure on Q04725
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003714 transcription corepressor activity
GO:0005515 protein binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0007165 signal transduction
GO:0009887 animal organ morphogenesis
GO:0016055 Wnt signaling pathway
GO:0045892 negative regulation of transcription, DNA-templated
GO:0090090 negative regulation of canonical Wnt signaling pathway
GO:1904837 beta-catenin-TCF complex assembly

Cellular Component:
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005925 focal adhesion
GO:0016604 nuclear body


-  Descriptions from all associated GenBank mRNAs
  BC017364 - Homo sapiens transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:10518 IMAGE:3925626), complete cds.
AK308137 - Homo sapiens cDNA, FLJ98085.
AK293482 - Homo sapiens cDNA FLJ61693 complete cds, highly similar to Transducin-like enhancer protein 2.
AK293407 - Homo sapiens cDNA FLJ59666 complete cds, highly similar to Transducin-like enhancer protein 2.
M99436 - Human transducin-like enhancer protein (TLE2) mRNA, complete cds.
AK312285 - Homo sapiens cDNA, FLJ92587.
JF432328 - Synthetic construct Homo sapiens clone IMAGE:100073514 transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2) gene, encodes complete protein.
KJ892283 - Synthetic construct Homo sapiens clone ccsbBroadEn_01677 TLE2 gene, encodes complete protein.
AK303102 - Homo sapiens cDNA FLJ53117 complete cds, highly similar to Transducin-like enhancer protein 2.
AK308608 - Homo sapiens cDNA, FLJ98649.
AK123183 - Homo sapiens cDNA FLJ41188 fis, clone BRACE2044304, highly similar to TRANSDUCIN-LIKE ENHANCER PROTEIN 2.
JD272474 - Sequence 253498 from Patent EP1572962.
JD307538 - Sequence 288562 from Patent EP1572962.
JD423755 - Sequence 404779 from Patent EP1572962.
JD376982 - Sequence 358006 from Patent EP1572962.
JD086349 - Sequence 67373 from Patent EP1572962.
CU675966 - Synthetic construct Homo sapiens gateway clone IMAGE:100020451 5' read TLE2 mRNA.
LF212346 - JP 2014500723-A/19849: Polycomb-Associated Non-Coding RNAs.
JD431661 - Sequence 412685 from Patent EP1572962.
JD381800 - Sequence 362824 from Patent EP1572962.
JD341687 - Sequence 322711 from Patent EP1572962.
MA447923 - JP 2018138019-A/19849: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q04725 (Reactome details) participates in the following event(s):

R-HSA-1912359 HES1 binds TLE
R-NUL-2065510 rHes1 binds TLE
R-HSA-4641229 TLE oligomerizes
R-HSA-4649028 TLE forms non-functional complexes with AES
R-HSA-3322431 XIAP binds TLE
R-HSA-8944349 TLE tetramers bind TCF/LEF at WNT promoters
R-HSA-3299569 Beta-catenin displaces TLE:HDAC1 from TCF/LEF
R-HSA-3322434 XIAP dissociates from ub-TLE
R-HSA-3322429 XIAP monoubiquinates TLE
R-HSA-4641231 TLE recruits HDAC1 to WNT promoters
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-4641265 Repression of WNT target genes
R-HSA-3769402 Deactivation of the beta-catenin transactivating complex
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-1980143 Signaling by NOTCH1
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-157118 Signaling by NOTCH
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: NM_003260, NP_003251, Q04725, Q8WVY0, Q9Y6S0, TLE2_HUMAN
UCSC ID: uc002lww.3
RefSeq Accession: NM_003260
Protein: Q04725 (aka TLE2_HUMAN)
CCDS: CCDS45911.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_003260.4
exon count: 20CDS single in 3' UTR: no RNA size: 2722
ORF size: 2232CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4140.00frame shift in genome: no % Coverage: 99.38
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.