Human Gene CEACAM5 (uc002orl.3)
  Description: Homo sapiens carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), mRNA.
RefSeq Summary (NM_004363): This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015].
Transcript (Including UTRs)
   Position: hg19 chr19:42,212,530-42,234,437 Size: 21,908 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr19:42,212,651-42,231,236 Size: 18,586 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:42,212,530-42,234,437)mRNA (may differ from genome)Protein (702 aa)
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MGIneXtProtOMIMPubMedReactomeTreefam
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CEAM5_HUMAN
DESCRIPTION: RecName: Full=Carcinoembryonic antigen-related cell adhesion molecule 5; AltName: Full=Carcinoembryonic antigen; Short=CEA; AltName: Full=Meconium antigen 100; AltName: CD_antigen=CD66e; Flags: Precursor;
FUNCTION: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
SUBUNIT: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
TISSUE SPECIFICITY: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
PTM: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
SIMILARITY: Belongs to the immunoglobulin superfamily. CEA family.
SIMILARITY: Contains 7 Ig-like (immunoglobulin-like) domains.
SEQUENCE CAUTION: Sequence=AAA62835.1; Type=Frameshift; Positions=359, 361;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CEACAM5
Diseases sorted by gene-association score: rectal neoplasm (11), colorectal cancer (10), lung cancer (9), gallbladder cancer (9), papillary adenocarcinoma (9), urachal cancer (8), pancreatic cancer (7), gastrointestinal carcinoma (6), urachal adenocarcinoma (6), cystadenocarcinoma (5), colon adenocarcinoma (5), gastric adenocarcinoma (5), ureter adenocarcinoma (5), immature teratoma of ovary (5), vaccinia (5), adamantinoid basal cell epithelioma (4), adamantinoma of long bones (4), stomach cancer (4), small cell cancer of the lung, somatic (4), mucoepidermoid carcinoma (4), medullary thyroid carcinoma, familial (3), hepatocellular carcinoma (3), colorectal adenocarcinoma (3), anal paget's disease (3), gallbladder lymphoma (3), lung cancer susceptibility 3 (3), prostate signet ring cell adenocarcinoma (3), cholangitis (3), mucoepidermoid esophageal carcinoma (3), anus adenocarcinoma (3), esophageal cancer (3), mesothelioma, somatic (3), cholangiocarcinoma, susceptibility to (2), apocrine adenoma (2), cutaneous mucoepidermoid carcinoma (2), sigmoid neoplasm (2), granulomatous endometritis (2), mucinous cystadenofibroma (2), female urethral cancer (2), gallbladder squamous cell carcinoma (2), breast cancer (2), bile duct cystadenocarcinoma (2), liver leiomyoma (2), warthin tumor (2), rectum adenocarcinoma (2), cell type cancer (2), gastrointestinal system cancer (2), cutaneous adenocystic carcinoma (2), liver lymphoma (2), ovarian cystadenocarcinoma (2), breast mucoepidermoid carcinoma (2), eccrine acrospiroma (2), tracheal cancer (2), vulval paget's disease (2), pancreatic serous cystadenoma (2), intracystic papillary adenoma (2), gastric cancer, somatic (2), vulva adenocarcinoma (2), fox-fordyce disease (2), epididymis cancer (2), epididymis adenocarcinoma (2), intracranial chondrosarcoma (2), ovary adenocarcinoma (2), ovary serous adenocarcinoma (2), nodular hidradenoma (2), anal gland adenocarcinoma (2), bile duct adenocarcinoma (2), cervix endometriosis (2), splenic tuberculosis (2), adenosquamous gallbladder carcinoma (2), descending colon cancer (2), ovarian cancer, somatic (2), microinvasive gastric cancer (2), thymus adenocarcinoma (2), adenoid basal cell carcinoma (2), ovary epithelial cancer (2), malignant ovarian surface epithelial-stromal neoplasm (2), gastrointestinal system benign neoplasm (2), endocrine gland cancer (2), cardiomyopathy, familial hypertrophic, 9 (2), mucinous adenofibroma (2), secretory meningioma (2), pericardial tuberculosis (1), melanotic medulloblastoma (1), rete testis adenocarcinoma (1), aggressive digital papillary adenocarcinoma (1), malignant spiradenoma (1), chondroid chordoma (1), intestinal benign neoplasm (1), clear cell adenoma (1), gastric small cell carcinoma (1), thyroid cancer, nonmedullary, 2 (1), cell type benign neoplasm (1), mucocele of salivary gland (1), lymphohistiocytoid mesothelioma (1), eccrine adenocarcinoma (1), organ system benign neoplasm (1), atypical follicular adenoma (1), pancreas adenocarcinoma (1), liver sarcoma (1), bile duct mucoepidermoid carcinoma (1), transverse colon cancer (1), integumentary system cancer (1), pancreatic mucinous cystadenoma (1), hematocele of tunica vaginalis testis (1), breast adenomyoepithelioma (1), breast myoepithelial neoplasm (1), female reproductive organ cancer (1), rete testis neoplasm (1), tuberculous empyema (1), epidermolysis bullosa, junctional, herlitz type (1), clear cell acanthoma (1), juvenile type testicular granulosa cell tumor (1), gastric signet ring cell adenocarcinoma (1), prostate squamous cell carcinoma (1), xanthogranulomatous cholecystitis (1), testicular granulosa cell tumor (1), melanoacanthoma (1), malignant epithelial mesothelioma (1), mucinous ovarian cystadenoma (1), biliary papillomatosis (1), ovarian lymphoma (1), ovarian mucinous neoplasm (1), colonic benign neoplasm (1), orbital cyst (1), mixed ductal-endocrine carcinoma (1), tolosa-hunt syndrome (1), acute thyroiditis (1), breast mucinous carcinoma (1), breast squamous cell carcinoma (1), meibomian cyst (1), tuberculous peritonitis (1), femoral neuropathy (1), congenital epulis (1), malignant biphasic mesothelioma (1), miliaria (1), urethral benign neoplasm (1), cervical clear cell adenocarcinoma (1), spermatocele (1), chondroid lipoma (1), infiltrative basal cell carcinoma (1), extragonadal seminoma (1), superior mesenteric artery syndrome (1), holoprosencephaly 1 (1), testicular yolk sac tumor (1), pancreatic cystadenocarcinoma (1), cystitis cystica (1), cloacogenic carcinoma (1), breast metaplastic carcinoma (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 347.48 RPKM in Colon - Transverse
Total median expression: 607.06 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -29.55121-0.244 Picture PostScript Text
3' UTR -384.911370-0.281 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR013098 - Ig_I-set
IPR003599 - Ig_sub
IPR003598 - Ig_sub2
IPR013106 - Ig_V-set

Pfam Domains:
PF00047 - Immunoglobulin domain
PF07679 - Immunoglobulin I-set domain
PF07686 - Immunoglobulin V-set domain
PF13895 - Immunoglobulin domain
PF13927 - Immunoglobulin domain

SCOP Domains:
48726 - Immunoglobulin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1E07 - X-ray 2QSQ - X-ray MuPIT 2QST - X-ray MuPIT 2VER - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P06731
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0034235 GPI anchor binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity

Biological Process:
GO:0007156 homophilic cell adhesion via plasma membrane adhesion molecules
GO:0007157 heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
GO:0010832 negative regulation of myotube differentiation
GO:0034109 homotypic cell-cell adhesion
GO:0043066 negative regulation of apoptotic process
GO:0050900 leukocyte migration
GO:2000811 negative regulation of anoikis

Cellular Component:
GO:0005576 extracellular region
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0031225 anchored component of membrane
GO:0070062 extracellular exosome
GO:0071575 integral component of external side of plasma membrane


-  Descriptions from all associated GenBank mRNAs
  JD147700 - Sequence 128724 from Patent EP1572962.
M29540 - Human carcinoembryonic antigen mRNA (CEA), complete cds.
JD515355 - Sequence 496379 from Patent EP1572962.
AK310844 - Homo sapiens cDNA, FLJ17886.
BC034671 - Homo sapiens carcinoembryonic antigen-related cell adhesion molecule 5, mRNA (cDNA clone MGC:34212 IMAGE:5184800), complete cds.
AK223101 - Homo sapiens mRNA for carcinoembryonic antigen-related cell adhesion molecule 5 variant, clone: KAT04486.
JD071438 - Sequence 52462 from Patent EP1572962.
JD153999 - Sequence 135023 from Patent EP1572962.
JD164612 - Sequence 145636 from Patent EP1572962.
CR749337 - Homo sapiens mRNA; cDNA DKFZp781M2392 (from clone DKFZp781M2392).
JD340028 - Sequence 321052 from Patent EP1572962.
DQ896753 - Synthetic construct Homo sapiens clone IMAGE:100011213; FLH166370.01L; RZPDo839G0185D carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) gene, encodes complete protein.
DQ890840 - Synthetic construct clone IMAGE:100003470; FLH166374.01X; RZPDo839G0186D carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) gene, encodes complete protein.
CU689928 - Synthetic construct Homo sapiens gateway clone IMAGE:100020345 5' read CEACAM5 mRNA.
KJ905171 - Synthetic construct Homo sapiens clone ccsbBroadEn_14581 CEACAM5 gene, encodes complete protein.
AB852566 - Homo sapiens CEACAM5 mRNA for carcinoembryonic antigen-related cell adhesion molecule 5 isoform 5D, complete cds.
AB852567 - Homo sapiens CEACAM5 mRNA for carcinoembryonic antigen-related cell adhesion molecule 5 isoform 3D, complete cds.
X16455 - Human mRNA for carcinoembryonic antigen pCEA80-11.
M15042 - Human carcinoembryonic antigen mRNA.
E01630 - cDNA encoding human carcinoembryonic antigen.
LF366910 - JP 2014500723-A/174413: Polycomb-Associated Non-Coding RNAs.
M17191 - Human carcinoembronyic antigen mRNA, partial cds.
M16234 - Human carcinoembryonic antigen mRNA, 3' end.
JD273129 - Sequence 254153 from Patent EP1572962.
JD351457 - Sequence 332481 from Patent EP1572962.
JD522416 - Sequence 503440 from Patent EP1572962.
JD320241 - Sequence 301265 from Patent EP1572962.
JD111652 - Sequence 92676 from Patent EP1572962.
JD145808 - Sequence 126832 from Patent EP1572962.
JD508173 - Sequence 489197 from Patent EP1572962.
JD334116 - Sequence 315140 from Patent EP1572962.
JD158749 - Sequence 139773 from Patent EP1572962.
JD208602 - Sequence 189626 from Patent EP1572962.
JD275630 - Sequence 256654 from Patent EP1572962.
JD411820 - Sequence 392844 from Patent EP1572962.
JD410069 - Sequence 391093 from Patent EP1572962.
JD165700 - Sequence 146724 from Patent EP1572962.
JD139892 - Sequence 120916 from Patent EP1572962.
JD136275 - Sequence 117299 from Patent EP1572962.
JD136276 - Sequence 117300 from Patent EP1572962.
JD243312 - Sequence 224336 from Patent EP1572962.
JD243313 - Sequence 224337 from Patent EP1572962.
JD125756 - Sequence 106780 from Patent EP1572962.
JD401790 - Sequence 382814 from Patent EP1572962.
JD176424 - Sequence 157448 from Patent EP1572962.
JD382764 - Sequence 363788 from Patent EP1572962.
JD351565 - Sequence 332589 from Patent EP1572962.
JD522743 - Sequence 503767 from Patent EP1572962.
JD139799 - Sequence 120823 from Patent EP1572962.
JD451539 - Sequence 432563 from Patent EP1572962.
JD364613 - Sequence 345637 from Patent EP1572962.
JD149742 - Sequence 130766 from Patent EP1572962.
JD149743 - Sequence 130767 from Patent EP1572962.
JD518761 - Sequence 499785 from Patent EP1572962.
JD298516 - Sequence 279540 from Patent EP1572962.
JD290645 - Sequence 271669 from Patent EP1572962.
JD317791 - Sequence 298815 from Patent EP1572962.
JD087417 - Sequence 68441 from Patent EP1572962.
JD503917 - Sequence 484941 from Patent EP1572962.
JD177463 - Sequence 158487 from Patent EP1572962.
MA602487 - JP 2018138019-A/174413: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P06731 (Reactome details) participates in the following event(s):

R-HSA-8867135 CEACAM1,3,5,6 bind opa proteins
R-HSA-8940388 GPLD1 hydrolyses GPI-anchors from proteins
R-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-109582 Hemostasis
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: CEA, CEAM5_HUMAN, NM_004363, NP_004354, P06731
UCSC ID: uc002orl.3
RefSeq Accession: NM_004363
Protein: P06731 (aka CEAM5_HUMAN or CEA5_HUMAN)
CCDS: CCDS12584.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_004363.2
exon count: 10CDS single in 3' UTR: no RNA size: 3600
ORF size: 2109CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4415.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.