Human Gene CBR3 (uc002yve.3)
  Description: Homo sapiens carbonyl reductase 3 (CBR3), mRNA.
RefSeq Summary (NM_001236): Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr21:37,507,263-37,518,860 Size: 11,598 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg19 chr21:37,507,491-37,518,810 Size: 11,320 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr21:37,507,263-37,518,860)mRNA (may differ from genome)Protein (277 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeTreefamUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CBR3_HUMAN
DESCRIPTION: RecName: Full=Carbonyl reductase [NADPH] 3; EC=1.1.1.184; AltName: Full=NADPH-dependent carbonyl reductase 3;
FUNCTION: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
CATALYTIC ACTIVITY: R-CHOH-R' + NADP(+) = R-CO-R' + NADPH.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=25 uM for menadione (PubMed:15537833); KM=43 uM for menadione (PubMed:18493841); KM=90 uM for NADPH (PubMed:15537833); KM=38 uM for NADPH (PubMed:18493841); pH dependence: Optimum pH is 5.5-7;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cbr3/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): CBR3
CDC HuGE Published Literature: CBR3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D000082 Acetaminophen
  • D001564 Benzo(a)pyrene
  • D024483 Vitamin K 3
  • C016766 sulforafan
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C028474 1,4-bis(2-(3,5-dichloropyridyloxy))benzene
  • C014211 2,3,7,8-tetrachlorodibenzofuran
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • C093973 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
  • C018855 2-tert-butylhydroquinone
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 30.49 RPKM in Esophagus - Mucosa
Total median expression: 339.36 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -110.50228-0.485 Picture PostScript Text
3' UTR -7.3250-0.146 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002198 - DH_sc/Rdtase_SDR
IPR002347 - Glc/ribitol_DH
IPR016040 - NAD(P)-bd_dom
IPR020904 - Sc_DH/Rdtase_CS

Pfam Domains:
PF00106 - short chain dehydrogenase
PF08659 - KR domain
PF13561 - Enoyl-(Acyl carrier protein) reductase

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2HRB - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O75828
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000253 3-keto sterol reductase activity
GO:0004090 carbonyl reductase (NADPH) activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0070402 NADPH binding

Biological Process:
GO:0006805 xenobiotic metabolic process
GO:0042376 phylloquinone catabolic process
GO:0050890 cognition
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  KX533495 - Homo sapiens mediator of cell motility 1 (Memo1) mRNA, complete cds.
AB041012 - Homo sapiens CBR3 mRNA for NADPH-dependent carbonyl reductase 3, complete cds.
AB124847 - Homo sapiens hCBR3 mRNA for carbonyl reductase 3, complete cds.
AK223429 - Homo sapiens mRNA for carbonyl reductase 3 variant, clone: FCC110G10.
BC002812 - Homo sapiens carbonyl reductase 3, mRNA (cDNA clone MGC:3489 IMAGE:3637434), complete cds.
JF432670 - Synthetic construct Homo sapiens clone IMAGE:100073908 carbonyl reductase 3 (CBR3) gene, encodes complete protein.
KJ896547 - Synthetic construct Homo sapiens clone ccsbBroadEn_05941 CBR3 gene, encodes complete protein.
KR709952 - Synthetic construct Homo sapiens clone CCSBHm_00008001 CBR3 (CBR3) mRNA, encodes complete protein.
CR541709 - Homo sapiens full open reading frame cDNA clone RZPDo834F1128D for gene CBR3, carbonyl reductase 3; complete cds, without stopcodon.
AB004854 - Homo sapiens mRNA for carbonyl reductase 3, complete cds.
FJ224313 - Homo sapiens epididymis secretory protein Li 25 (HEL-S-25) mRNA, complete cds.
AJ000095 - Homo sapiens mRNA for carbonyl reductase, clone p1079, partail.
AB004850 - Homo sapiens mRNA expressed in lung fibroblast, clone IMAGE:529844.
JD094223 - Sequence 75247 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00590 - Arachidonic acid metabolism
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein O75828 (Reactome details) participates in the following event(s):

R-HSA-8937419 CBR3 reduces DOX to DOXOL
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: CBR3_HUMAN, NM_001236, NP_001227, O75828, Q6FHP2
UCSC ID: uc002yve.3
RefSeq Accession: NM_001236
Protein: O75828 (aka CBR3_HUMAN)
CCDS: CCDS13642.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001236.3
exon count: 3CDS single in 3' UTR: no RNA size: 1128
ORF size: 834CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1868.00frame shift in genome: no % Coverage: 98.58
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.