Human Gene ARHGAP8 (uc003bfk.3)
  Description: Homo sapiens Rho GTPase activating protein 8 (ARHGAP8), transcript variant 2, mRNA.
RefSeq Summary (NM_181335): This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010].
Transcript (Including UTRs)
   Position: hg19 chr22:45,148,438-45,258,664 Size: 110,227 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr22:45,182,408-45,258,475 Size: 76,068 Coding Exon Count: 11 

Page IndexSequence and LinksPrimersGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:45,148,438-45,258,664)mRNA (may differ from genome)Protein (433 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCLynxMalacardsMGIOMIM
PubMedReactomeTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): ARHGAP8
CDC HuGE Published Literature: ARHGAP8
Positive Disease Associations: monocyte chemoattractant protein 1 (66-77)
Related Studies:
  1. monocyte chemoattractant protein 1 (66-77)
    , , . [PubMed 0]

-  MalaCards Disease Associations
  MalaCards Gene Search: ARHGAP8
Diseases sorted by gene-association score: cervix carcinoma (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.69 RPKM in Spleen
Total median expression: 3.36 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -66.50141-0.472 Picture PostScript Text
3' UTR -42.74189-0.226 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF00620 - RhoGAP domain
PF00650 - CRAL/TRIO domain
PF13716 - Divergent CRAL/TRIO domain

SCOP Domains:
48350 - GTPase activation domain, GAP
52087 - CRAL/TRIO domain

ModBase Predicted Comparative 3D Structure on P85298-4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Descriptions from all associated GenBank mRNAs
  AL355192 - Novel human gene mapping to chomosome 22.
AF195968 - Homo sapiens rho GTPase activating protein 8 isoform 1 (ARHGAP8) mRNA, complete cds.
BC132755 - Homo sapiens PRR5-ARHGAP8 fusion, mRNA (cDNA clone MGC:164386 IMAGE:40146777), complete cds.
BC132757 - Homo sapiens PRR5-ARHGAP8 fusion, mRNA (cDNA clone MGC:164388 IMAGE:40146779), complete cds.
BC144382 - Homo sapiens PRR5-ARHGAP8 fusion, mRNA (cDNA clone MGC:177920 IMAGE:9052903), complete cds.
HQ258425 - Synthetic construct Homo sapiens clone IMAGE:100072854 PRR5-ARHGAP8 readthrough (PRR5-ARHGAP8) (PRR5-ARHGAP8) gene, encodes complete protein.
KJ904318 - Synthetic construct Homo sapiens clone ccsbBroadEn_13712 PRR5-ARHGAP8 gene, encodes complete protein.
AK001306 - Homo sapiens cDNA FLJ10444 fis, clone NT2RP1000825, moderately similar to GTPASE-ACTIVATING PROTEIN RHOGAP.
BC059382 - Homo sapiens Rho GTPase activating protein 8, mRNA (cDNA clone IMAGE:30336727), partial cds.
BC067824 - Homo sapiens cDNA clone IMAGE:30339826, containing frame-shift errors.
BC048280 - Homo sapiens Rho GTPase activating protein 8, mRNA (cDNA clone MGC:54201 IMAGE:5190702), complete cds.
AK022305 - Homo sapiens cDNA FLJ12243 fis, clone MAMMA1001305, moderately similar to RHO-GTPASE-ACTIVATING PROTEIN 1.
BC010490 - Homo sapiens Rho GTPase activating protein 8, mRNA (cDNA clone IMAGE:2900625), partial cds.
AB590903 - Synthetic construct DNA, clone: pFN21AE1927, Homo sapiens ARHGAP8 gene for Rho GTPase activating protein 8, without stop codon, in Flexi system.
KJ898538 - Synthetic construct Homo sapiens clone ccsbBroadEn_07932 ARHGAP8 gene, encodes complete protein.
AF544240 - Homo sapiens BCH domain-containing Cdc42GAP-like protein (BPGAP1) mRNA, complete cds.
AF195969 - Homo sapiens rho GTPase activating protein 8 isoform 2 (AHRGAP8) mRNA, complete cds.
JD437433 - Sequence 418457 from Patent EP1572962.
JD211273 - Sequence 192297 from Patent EP1572962.
JD407380 - Sequence 388404 from Patent EP1572962.
JD119659 - Sequence 100683 from Patent EP1572962.
JD137836 - Sequence 118860 from Patent EP1572962.
JD239619 - Sequence 220643 from Patent EP1572962.
JD258372 - Sequence 239396 from Patent EP1572962.
JD372024 - Sequence 353048 from Patent EP1572962.
JD129837 - Sequence 110861 from Patent EP1572962.
JD200200 - Sequence 181224 from Patent EP1572962.
JD143926 - Sequence 124950 from Patent EP1572962.
JD141094 - Sequence 122118 from Patent EP1572962.
JD402221 - Sequence 383245 from Patent EP1572962.
BC111953 - Homo sapiens Rho GTPase activating protein 8, mRNA (cDNA clone IMAGE:8327421), partial cds.
BC093675 - Homo sapiens Rho GTPase activating protein 8, mRNA (cDNA clone IMAGE:7939520), partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P85298 (Reactome details) participates in the following event(s):

R-HSA-194922 GAPs inactivate Rho GTPase:GTP by hydrolysis
R-HSA-194840 Rho GTPase cycle
R-HSA-194315 Signaling by Rho GTPases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: NM_181335, NP_851852, P85298-4
UCSC ID: uc003bfk.3
RefSeq Accession: NM_181335
Protein: P85298-4, splice isoform of P85298 CCDS: CCDS14060.2

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_181335.2
exon count: 12CDS single in 3' UTR: no RNA size: 1632
ORF size: 1302CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2750.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.