Human Gene TOPAZ1 (uc003cna.4)
  Description: Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr3:44,283,378-44,373,590 Size: 90,213 Total Exon Count: 20 Strand: +
Coding Region
   Position: hg19 chr3:44,283,546-44,373,503 Size: 89,958 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:44,283,378-44,373,590)mRNA (may differ from genome)Protein (1692 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TOPZ1_HUMAN
DESCRIPTION: RecName: Full=Testis- and ovary-specific PAZ domain-containing protein 1;
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity).
SIMILARITY: Contains 1 C3H1-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 2.95 RPKM in Testis
Total median expression: 3.02 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -87.20168-0.519 Picture PostScript Text
3' UTR -10.5087-0.121 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF14669 - Putative aspartate racemase

ModBase Predicted Comparative 3D Structure on Q8N9V7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0006915 apoptotic process
GO:0007283 spermatogenesis
GO:0007286 spermatid development
GO:0030154 cell differentiation
GO:0048137 spermatocyte division
GO:0098781 ncRNA transcription
GO:1901995 positive regulation of meiotic cell cycle phase transition

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BX648094 - Homo sapiens mRNA; cDNA DKFZp686M1132 (from clone DKFZp686M1132).
AK093476 - Homo sapiens cDNA FLJ36157 fis, clone TESTI2025656.
AX748152 - Sequence 1677 from Patent EP1308459.

-  Other Names for This Gene
  Alternate Gene Symbols: C3orf77, NM_001145030, NP_001138502, Q8N9V7, TOPZ1_HUMAN
UCSC ID: uc003cna.4
RefSeq Accession: NM_001145030
Protein: Q8N9V7 (aka TOPZ1_HUMAN)
CCDS: CCDS46809.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001145030.1
exon count: 20CDS single in 3' UTR: no RNA size: 5351
ORF size: 5079CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 10243.00frame shift in genome: no % Coverage: 99.68
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.