Human Gene HMGN3 (uc003piu.2)
  Description: Homo sapiens high mobility group nucleosomal binding domain 3 (HMGN3), transcript variant 3, mRNA.
RefSeq Summary (NM_001201362): The protein encoded by this gene binds thyroid hormone receptor beta in the presence of thyroid hormone. The encoded protein, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is a related pseudogene on chromosome 1. [provided by RefSeq, Jan 2016].
Transcript (Including UTRs)
   Position: hg19 chr6:79,910,962-79,944,455 Size: 33,494 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr6:79,911,966-79,944,277 Size: 32,312 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:79,910,962-79,944,455)mRNA (may differ from genome)Protein (95 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HMGN3_HUMAN
DESCRIPTION: RecName: Full=High mobility group nucleosome-binding domain-containing protein 3; AltName: Full=Thyroid receptor-interacting protein 7; Short=TR-interacting protein 7; Short=TRIP-7;
FUNCTION: Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function (By similarity).
SUBUNIT: Interacts with the ligand binding domain of the thyroid receptor (TR) (in vitro). Requires the presence of thyroid hormone for its interaction. Interacts with nucleosomes.
INTERACTION: P62993:GRB2; NbExp=1; IntAct=EBI-1758705, EBI-401755;
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries.
INDUCTION: By estrogen.
SIMILARITY: Belongs to the HMGN family.
SEQUENCE CAUTION: Sequence=AAA73877.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 131.18 RPKM in Pituitary
Total median expression: 3524.47 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -76.60178-0.430 Picture PostScript Text
3' UTR -245.361004-0.244 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000079 - HMGN_fam

Pfam Domains:
PF01101 - HMG14 and HMG17

ModBase Predicted Comparative 3D Structure on Q15651
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0031492 nucleosomal DNA binding
GO:0046966 thyroid hormone receptor binding

Biological Process:
GO:0006325 chromatin organization
GO:0008150 biological_process
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0061178 regulation of insulin secretion involved in cellular response to glucose stimulus

Cellular Component:
GO:0000785 chromatin
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AY043282 - Homo sapiens TRIP7-like protein mRNA, complete cds.
BX648085 - Homo sapiens mRNA; cDNA DKFZp686E20226 (from clone DKFZp686E20226).
AK124181 - Homo sapiens cDNA FLJ42187 fis, clone THYMU2032358.
AF401520 - Homo sapiens TRIP7 variant protein mRNA, complete cds, alternatively spliced.
BC009529 - Homo sapiens high mobility group nucleosomal binding domain 3, mRNA (cDNA clone MGC:10048 IMAGE:3890883), complete cds.
AF274949 - Homo sapiens PNAS-24 mRNA, complete cds.
JD546519 - Sequence 527543 from Patent EP1572962.
JD237960 - Sequence 218984 from Patent EP1572962.
JD093509 - Sequence 74533 from Patent EP1572962.
JD237272 - Sequence 218296 from Patent EP1572962.
L40357 - Homo sapiens thyroid receptor interactor (TRIP7) mRNA, 3' end of cds.
JD092955 - Sequence 73979 from Patent EP1572962.
JD316482 - Sequence 297506 from Patent EP1572962.
AK315391 - Homo sapiens cDNA, FLJ96439, Homo sapiens high mobility group nucleosomal binding domain 3(HMGN3), transcript variant 2, mRNA.
KJ892745 - Synthetic construct Homo sapiens clone ccsbBroadEn_02139 HMGN3 gene, encodes complete protein.
JD205487 - Sequence 186511 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2RD37, HMGN3_HUMAN, NM_001201362, NP_001188291, PNAS-24, Q15651, Q5HYD3, Q7RTT0, Q969M5, Q9BZT7, TRIP7
UCSC ID: uc003piu.2
RefSeq Accession: NM_001201362
Protein: Q15651 (aka HMGN3_HUMAN or HMN3_HUMAN)

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001201362.1
exon count: 5CDS single in 3' UTR: no RNA size: 1484
ORF size: 288CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 776.00frame shift in genome: no % Coverage: 99.06
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 1031# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.