Human Gene MAPK15 (uc003yzj.3)
  Description: Homo sapiens mitogen-activated protein kinase 15 (MAPK15), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr8:144,798,507-144,804,633 Size: 6,127 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr8:144,798,548-144,804,421 Size: 5,874 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:144,798,507-144,804,633)mRNA (may differ from genome)Protein (544 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedTreefamUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MK15_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase 15; Short=MAP kinase 15; Short=MAPK 15; EC=2.7.11.24; AltName: Full=Extracellular signal-regulated kinase 7; Short=ERK-7; AltName: Full=Extracellular signal-regulated kinase 8; Short=ERK-8;
FUNCTION: In vitro, phosphorylates MBP.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1 (By similarity).
SUBUNIT: Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.
TISSUE SPECIFICITY: Widely expressed with a maximal expression in lung and kidney.
DOMAIN: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation (By similarity).
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
PTM: Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.05 RPKM in Testis
Total median expression: 119.06 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.3041-0.202 Picture PostScript Text
3' UTR -57.30212-0.270 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR003527 - MAP_kinase_CS
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q8TD08
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details  Gene DetailsGene Details 
Gene Sorter  Gene SorterGene Sorter 
  EnsemblFlyBaseWormBase 
  Protein SequenceProtein SequenceProtein Sequence 
  AlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004707 MAP kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0017124 SH3 domain binding

Biological Process:
GO:0000165 MAPK cascade
GO:0003400 regulation of COPII vesicle coating
GO:0006468 protein phosphorylation
GO:0007029 endoplasmic reticulum organization
GO:0010468 regulation of gene expression
GO:0010506 regulation of autophagy
GO:0016310 phosphorylation
GO:0030336 negative regulation of cell migration
GO:0032212 positive regulation of telomere maintenance via telomerase
GO:0046777 protein autophosphorylation
GO:0051973 positive regulation of telomerase activity
GO:0090494 dopamine uptake
GO:1902017 regulation of cilium assembly
GO:1904355 positive regulation of telomere capping
GO:1904491 protein localization to ciliary transition zone
GO:1905188 positive regulation of metaphase/anaphase transition of meiosis I

Cellular Component:
GO:0005576 extracellular region
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005776 autophagosome
GO:0005794 Golgi apparatus
GO:0005814 centriole
GO:0005911 cell-cell junction
GO:0036064 ciliary basal body
GO:0072687 meiotic spindle
GO:0005930 axoneme


-  Descriptions from all associated GenBank mRNAs
  BC028034 - Homo sapiens mitogen-activated protein kinase 15, mRNA (cDNA clone MGC:40087 IMAGE:5242481), complete cds.
AY065978 - Homo sapiens extracellular signal-regulated kinase 8 mRNA, complete cds.
AB587543 - Synthetic construct DNA, clone: pF1KE0975, Homo sapiens MAPK15 gene for mitogen-activated protein kinase 15, without stop codon, in Flexi system.
DQ891473 - Synthetic construct clone IMAGE:100004103; FLH177300.01X; RZPDo839F12124D mitogen-activated protein kinase 15 (MAPK15) gene, encodes complete protein.
DQ894661 - Synthetic construct Homo sapiens clone IMAGE:100009121; FLH177297.01L; RZPDo839F12123D mitogen-activated protein kinase 15 (MAPK15) gene, encodes complete protein.
CU688372 - Synthetic construct Homo sapiens gateway clone IMAGE:100016616 5' read MAPK15 mRNA.
KJ904048 - Synthetic construct Homo sapiens clone ccsbBroadEn_13442 MAPK15 gene, encodes complete protein.
KJ905650 - Synthetic construct Homo sapiens clone ccsbBroadEn_15292 MAPK15 gene, encodes complete protein.
AY994058 - Homo sapiens extracellular regulated kinase 8 delta (MAPK15) mRNA, complete cds, alternatively spliced.
JD239930 - Sequence 220954 from Patent EP1572962.
JD552137 - Sequence 533161 from Patent EP1572962.
JD449232 - Sequence 430256 from Patent EP1572962.
JD088638 - Sequence 69662 from Patent EP1572962.
JD267549 - Sequence 248573 from Patent EP1572962.
JD440632 - Sequence 421656 from Patent EP1572962.
JD191983 - Sequence 173007 from Patent EP1572962.
JD051243 - Sequence 32267 from Patent EP1572962.
JD064862 - Sequence 45886 from Patent EP1572962.
JD106678 - Sequence 87702 from Patent EP1572962.
JD481748 - Sequence 462772 from Patent EP1572962.
JD444486 - Sequence 425510 from Patent EP1572962.
JD338808 - Sequence 319832 from Patent EP1572962.
JD264668 - Sequence 245692 from Patent EP1572962.
JD265409 - Sequence 246433 from Patent EP1572962.
JD498841 - Sequence 479865 from Patent EP1572962.
JD074198 - Sequence 55222 from Patent EP1572962.
JD142001 - Sequence 123025 from Patent EP1572962.
JD112880 - Sequence 93904 from Patent EP1572962.
JD508338 - Sequence 489362 from Patent EP1572962.
JD192192 - Sequence 173216 from Patent EP1572962.
JD483987 - Sequence 465011 from Patent EP1572962.
JD215797 - Sequence 196821 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ERK7, ERK8, MK15_HUMAN, NM_139021, NP_620590, Q2TCF9, Q8N362, Q8TD08
UCSC ID: uc003yzj.3
RefSeq Accession: NM_139021
Protein: Q8TD08 (aka MK15_HUMAN)
CCDS: CCDS6409.2

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_139021.2
exon count: 14CDS single in 3' UTR: no RNA size: 1904
ORF size: 1635CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3293.00frame shift in genome: no % Coverage: 99.16
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.