Description: Homo sapiens regucalcin (senescence marker protein-30) (RGN), transcript variant 1, mRNA. RefSeq Summary (NM_004683): The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]. Transcript (Including UTRs) Position: hg19 chrX:46,937,775-46,952,712 Size: 14,938 Total Exon Count: 7 Strand: + Coding Region Position: hg19 chrX:46,940,544-46,952,346 Size: 11,803 Coding Exon Count: 6
ID:RGN_HUMAN DESCRIPTION: RecName: Full=Regucalcin; Short=RC; AltName: Full=Gluconolactonase; Short=GNL; EC=3.1.1.17; AltName: Full=Senescence marker protein 30; Short=SMP-30; FUNCTION: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity). CATALYTIC ACTIVITY: D-glucono-1,5-lactone + H(2)O = D-gluconate. COFACTOR: Binds 1 divalent metal cation per subunit. Most active with zinc and manganese ions. The physiological cofactor is most likely calcium or magnesium. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.7 mM for gluconolactone (with Zn(2+) as cofactor); KM=0.6 mM for gluconolactone (with Mn(2+) as cofactor); KM=1.3 mM for gluconolactone (with Mg(2+) as cofactor); KM=3.7 mM for gluconolactone (with Ca(2+) as cofactor); SUBCELLULAR LOCATION: Cytoplasm (By similarity). SIMILARITY: Belongs to the SMP-30/CGR1 family. CAUTION: Gluconolactonase catalyzes a key step in ascorbic acid (vitamin C) biosynthesis, but primates lack the last enzyme in the pathway and are unable to synthesize vitamin C.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q15493
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001822 kidney development GO:0001889 liver development GO:0001933 negative regulation of protein phosphorylation GO:0006469 negative regulation of protein kinase activity GO:0006874 cellular calcium ion homeostasis GO:0007283 spermatogenesis GO:0007568 aging GO:0010867 positive regulation of triglyceride biosynthetic process GO:0010907 positive regulation of glucose metabolic process GO:0010922 positive regulation of phosphatase activity GO:0019853 L-ascorbic acid biosynthetic process GO:0032515 negative regulation of phosphoprotein phosphatase activity GO:0032781 positive regulation of ATPase activity GO:0034260 negative regulation of GTPase activity GO:0043066 negative regulation of apoptotic process GO:0043547 positive regulation of GTPase activity GO:0045019 negative regulation of nitric oxide biosynthetic process GO:0045723 positive regulation of fatty acid biosynthetic process GO:0050680 negative regulation of epithelial cell proliferation GO:0050848 regulation of calcium-mediated signaling GO:0051344 negative regulation of cyclic-nucleotide phosphodiesterase activity GO:0097421 liver regeneration GO:1901318 negative regulation of flagellated sperm motility GO:1901671 positive regulation of superoxide dismutase activity GO:1901896 positive regulation of calcium-transporting ATPase activity GO:1902679 negative regulation of RNA biosynthetic process GO:1903011 negative regulation of bone development GO:1903052 positive regulation of proteolysis involved in cellular protein catabolic process GO:1903611 negative regulation of calcium-dependent ATPase activity GO:1903625 negative regulation of DNA catabolic process GO:1903629 positive regulation of dUTP diphosphatase activity GO:1903634 negative regulation of leucine-tRNA ligase activity GO:2000279 negative regulation of DNA biosynthetic process
US Server
