Description: Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 1, mRNA. RefSeq Summary (NM_002605): The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript (Including UTRs) Position: hg19 chr15:85,525,205-85,682,376 Size: 157,172 Total Exon Count: 23 Strand: + Coding Region Position: hg19 chr15:85,634,302-85,681,134 Size: 46,833 Coding Exon Count: 15
ID:PDE8A_HUMAN DESCRIPTION: RecName: Full=High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; EC=3.1.4.17; FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate. COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and zaprinast as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. TISSUE SPECIFICITY: Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon. DOMAIN: Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain. SIMILARITY: Contains 1 PAS (PER-ARNT-SIM) domain. SEQUENCE CAUTION: Sequence=AAL18612.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18613.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18614.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=BAG54458.1; Type=Miscellaneous discrepancy; Note=Intron retention; Sequence=EAX01967.1; Type=Erroneous gene model prediction;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60658
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001934 positive regulation of protein phosphorylation GO:0006198 cAMP catabolic process GO:0006355 regulation of transcription, DNA-templated GO:0007165 signal transduction GO:0007186 G-protein coupled receptor signaling pathway GO:0009187 cyclic nucleotide metabolic process GO:0060548 negative regulation of cell death GO:0070374 positive regulation of ERK1 and ERK2 cascade GO:0071364 cellular response to epidermal growth factor stimulus GO:1903206 negative regulation of hydrogen peroxide-induced cell death