Human Gene DPEP3 (uc010cex.3)
  Description: Homo sapiens dipeptidase 3 (DPEP3), transcript variant 2, mRNA.
RefSeq Summary (NM_001129758): This gene encodes a membrane-bound glycoprotein from the family of dipeptidases involved in hydrolytic metabolism of various dipeptides, including penem and carbapenem beta-lactam antibiotics. This gene is located on chromosome 16 in a cluster with another member of this family. Alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr16:68,009,566-68,014,452 Size: 4,887 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr16:68,009,668-68,014,358 Size: 4,691 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:68,009,566-68,014,452)mRNA (may differ from genome)Protein (512 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeTreefamUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DPEP3_HUMAN
DESCRIPTION: RecName: Full=Dipeptidase 3; EC=3.4.13.19; Flags: Precursor;
FUNCTION: Probable metalloprotease which hydrolyzes cystinyl-bis- glycine. May be involved in meiosis (By similarity).
CATALYTIC ACTIVITY: Hydrolysis of dipeptides.
COFACTOR: Zinc (By similarity).
ENZYME REGULATION: Inhibited by L-penicillamine (By similarity).
SUBUNIT: Homodimer; disulfide-linked (By similarity).
SUBCELLULAR LOCATION: Membrane; Lipid-anchor, GPI-anchor (By similarity).
SIMILARITY: Belongs to the peptidase M19 family.
SEQUENCE CAUTION: Sequence=AAH57789.1; Type=Erroneous initiation; Sequence=BAG51910.1; Type=Erroneous initiation; Sequence=CAC15385.1; Type=Erroneous initiation; Sequence=EAW83198.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): DPEP3
CDC HuGE Published Literature: DPEP3
Positive Disease Associations: Cholesterol, HDL , Lipoproteins, HDL
Related Studies:
  1. Cholesterol, HDL
    Chiara Sabatti et al. Nature genetics 2009, Genome-wide association analysis of metabolic traits in a birth cohort from a founder population., Nature genetics. [PubMed 19060910]
  2. Lipoproteins, HDL
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 53.20 RPKM in Testis
Total median expression: 59.43 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.6094-0.411 Picture PostScript Text
3' UTR -18.80102-0.184 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000180 - Pept_M19_AS
IPR008257 - Peptidase_M19

Pfam Domains:
PF01244 - Membrane dipeptidase (Peptidase family M19)

SCOP Domains:
51556 - Metallo-dependent hydrolases

ModBase Predicted Comparative 3D Structure on Q9H4B8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008233 peptidase activity
GO:0008235 metalloexopeptidase activity
GO:0008237 metallopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0016787 hydrolase activity
GO:0016805 dipeptidase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0007140 male meiosis
GO:0051321 meiotic cell cycle

Cellular Component:
GO:0001669 acrosomal vesicle
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031225 anchored component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC037243 - Homo sapiens dipeptidase 3, mRNA (cDNA clone IMAGE:5171919).
AJ291679 - Homo sapiens mRNA for putative metallopeptidase (family M19).
AY358390 - Homo sapiens clone DNA59817 QPTG834 (UNQ834) mRNA, complete cds.
HM005556 - Homo sapiens clone HTL-S-16 testis secretory sperm-binding protein Li 211a mRNA, complete cds.
LP896040 - Sequence 904 from Patent EP3253886.
AK057401 - Homo sapiens cDNA FLJ32839 fis, clone TESTI2003311, highly similar to Dipeptidase 3 precursor (EC 3.4.13.19).
BC057789 - Homo sapiens dipeptidase 3, mRNA (cDNA clone MGC:71629 IMAGE:5297958), complete cds.
JD309806 - Sequence 290830 from Patent EP1572962.
JD061217 - Sequence 42241 from Patent EP1572962.
KJ903064 - Synthetic construct Homo sapiens clone ccsbBroadEn_12458 DPEP3 gene, encodes complete protein.
AB590471 - Synthetic construct DNA, clone: pFN21AE1957, Homo sapiens DPEP3 gene for dipeptidase 3, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H4B8 (Reactome details) participates in the following event(s):

R-HSA-266012 LTD4 is converted to LTE4 by DPEP1/2
R-HSA-5433067 DPEPs hydrolyse glycine from AFXBO-CG, AFNBO-CG
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-211859 Biological oxidations
R-HSA-8978868 Fatty acid metabolism
R-HSA-1430728 Metabolism
R-HSA-556833 Metabolism of lipids

-  Other Names for This Gene
  Alternate Gene Symbols: B3KQ48, DPEP3_HUMAN, NM_001129758, NP_001123230, Q6PEZ5, Q6UXE4, Q9H4B8, UNQ834/PRO1772
UCSC ID: uc010cex.3
RefSeq Accession: NM_001129758
Protein: Q9H4B8 (aka DPEP3_HUMAN)
CCDS: CCDS10856.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001129758.1
exon count: 10CDS single in 3' UTR: no RNA size: 1753
ORF size: 1539CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3278.00frame shift in genome: no % Coverage: 98.97
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.