Human Gene TLE2 (uc010dti.3)
  Description: Homo sapiens transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2, mRNA.
Transcript (Including UTRs)
   Position: hg19 chr19:2,997,636-3,047,633 Size: 49,998 Total Exon Count: 20 Strand: -
Coding Region
   Position: hg19 chr19:2,997,922-3,045,786 Size: 47,865 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:2,997,636-3,047,633)mRNA (may differ from genome)Protein (706 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsH-INV
HGNCLynxMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: F8WCH2_HUMAN
DESCRIPTION: SubName: Full=Transducin-like enhancer protein 2;
CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): TLE2
CDC HuGE Published Literature: TLE2
Positive Disease Associations: Cholesterol , Cholesterol, LDL
Related Studies:
  1. Cholesterol
    , , . [PubMed 0]
  2. Cholesterol, LDL
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 198.06 RPKM in Brain - Cerebellum
Total median expression: 1331.22 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.8184-0.176 Picture PostScript Text
3' UTR -97.20286-0.340 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005617 - Groucho/TLE_N
IPR009146 - Groucho_enhance
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat
PF03920 - Groucho/TLE N-terminal Q-rich domain

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Prolyl oligopeptidase, N-terminal domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - Dipeptidyl peptidase IV/CD26, N-terminal domain

ModBase Predicted Comparative 3D Structure on F8WCH2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Descriptions from all associated GenBank mRNAs
  BC017364 - Homo sapiens transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:10518 IMAGE:3925626), complete cds.
AK308137 - Homo sapiens cDNA, FLJ98085.
AK293482 - Homo sapiens cDNA FLJ61693 complete cds, highly similar to Transducin-like enhancer protein 2.
AK293407 - Homo sapiens cDNA FLJ59666 complete cds, highly similar to Transducin-like enhancer protein 2.
M99436 - Human transducin-like enhancer protein (TLE2) mRNA, complete cds.
AK312285 - Homo sapiens cDNA, FLJ92587.
JF432328 - Synthetic construct Homo sapiens clone IMAGE:100073514 transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2) gene, encodes complete protein.
KJ892283 - Synthetic construct Homo sapiens clone ccsbBroadEn_01677 TLE2 gene, encodes complete protein.
AK303102 - Homo sapiens cDNA FLJ53117 complete cds, highly similar to Transducin-like enhancer protein 2.
AK308608 - Homo sapiens cDNA, FLJ98649.
AK123183 - Homo sapiens cDNA FLJ41188 fis, clone BRACE2044304, highly similar to TRANSDUCIN-LIKE ENHANCER PROTEIN 2.
JD272474 - Sequence 253498 from Patent EP1572962.
JD307538 - Sequence 288562 from Patent EP1572962.
JD423755 - Sequence 404779 from Patent EP1572962.
JD376982 - Sequence 358006 from Patent EP1572962.
JD086349 - Sequence 67373 from Patent EP1572962.
CU675966 - Synthetic construct Homo sapiens gateway clone IMAGE:100020451 5' read TLE2 mRNA.
LF212346 - JP 2014500723-A/19849: Polycomb-Associated Non-Coding RNAs.
MA447923 - JP 2018138019-A/19849: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: F8WCH2, F8WCH2_HUMAN, NM_001144761, NP_001138233
UCSC ID: uc010dti.3
RefSeq Accession: NM_001144761
Protein: F8WCH2 CCDS: CCDS45913.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001144761.1
exon count: 20CDS single in 3' UTR: no RNA size: 2508
ORF size: 2121CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3956.00frame shift in genome: no % Coverage: 99.32
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.