Human Gene STK16 (uc010fwf.3)
  Description: Homo sapiens serine/threonine kinase 16 (STK16), transcript variant 2, non-coding RNA.
Transcript (Including UTRs)
   Position: hg19 chr2:220,110,201-220,112,913 Size: 2,713 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr2:220,110,722-220,112,665 Size: 1,944 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:220,110,201-220,112,913)mRNA (may differ from genome)Protein (280 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
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MGIneXtProtOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: STK16_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase 16; EC=2.7.11.1; AltName: Full=Myristoylated and palmitoylated serine/threonine-protein kinase; Short=MPSK; AltName: Full=Protein kinase PKL12; AltName: Full=TGF-beta-stimulated factor 1; Short=TSF-1; AltName: Full=Tyrosine-protein kinase STK16; EC=2.7.10.2; AltName: Full=hPSK;
FUNCTION: Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.
INTERACTION: Q6UY14:ADAMTSL4; NbExp=3; IntAct=EBI-749295, EBI-742002;
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region. Membrane; Lipid-anchor (By similarity). Note=Associates with Golgi and Golgi-derived vesicles (By similarity).
TISSUE SPECIFICITY: Ubiquitously expressed at very low levels.
PTM: Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198.
PTM: It is uncertain whether palmitoylation is on Cys-6 and/or Cys-8.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAV38392.1; Type=Frameshift; Positions=305; Sequence=CAA06700.1; Type=Frameshift; Positions=305;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: STK16
Diseases sorted by gene-association score: diffuse mesangial sclerosis (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.00 RPKM in Testis
Total median expression: 671.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -201.41422-0.477 Picture PostScript Text
3' UTR -95.00248-0.383 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2BUJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O75716
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
 Gene Sorter    
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 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006366 transcription from RNA polymerase II promoter
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046777 protein autophosphorylation
GO:0071560 cellular response to transforming growth factor beta stimulus

Cellular Component:
GO:0005737 cytoplasm
GO:0005798 Golgi-associated vesicle
GO:0005829 cytosol
GO:0016020 membrane
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK292694 - Homo sapiens cDNA FLJ75960 complete cds, highly similar to Homo sapiens serine/threonine kinase 16 (STK16), transcript variant 2, mRNA.
AK310793 - Homo sapiens cDNA, FLJ17835.
AK298471 - Homo sapiens cDNA FLJ60771 complete cds, highly similar to Serine/threonine-protein kinase 16 (EC 2.7.11.1).
AK225526 - Homo sapiens mRNA for serine/threonine kinase 16 variant, clone: KAT06371.
AF060798 - Homo sapiens myristilated and palmitylated serine-threonine kinase MPSK (MPSK1) mRNA, complete cds.
BC002618 - Homo sapiens serine/threonine kinase 16, mRNA (cDNA clone MGC:1953 IMAGE:3143105), complete cds.
AF203910 - Homo sapiens serine/threonine kinase KRCT mRNA, complete cds.
AK225392 - Homo sapiens mRNA for serine/threonine kinase 16 variant, clone: HRC05788.
BC053998 - Homo sapiens serine/threonine kinase 16, mRNA (cDNA clone MGC:61516 IMAGE:5760866), complete cds.
AK096954 - Homo sapiens cDNA FLJ39635 fis, clone SMINT2002882, highly similar to Homo sapiens mRNA for F5-2.
AK302182 - Homo sapiens cDNA FLJ60057 complete cds, highly similar to Serine/threonine-protein kinase 16 (EC 2.7.11.1).
AJ005791 - Homo sapiens mRNA for PKL12 protein.
AB020739 - Homo sapiens mRNA for F5-2, complete cds.
AJ010872 - Homo sapiens mRNA for serine/threonine kinase.
CR407675 - Homo sapiens full open reading frame cDNA clone RZPDo834C043D for gene STK16, serine/threonine kinase 16 complete cds, without stopcodon.
CU691068 - Synthetic construct Homo sapiens gateway clone IMAGE:100021661 5' read STK16 mRNA.
EU832072 - Synthetic construct Homo sapiens clone HAIB:100067101; DKFZo004G0824 serine/threonine kinase 16 protein (STK16) gene, encodes complete protein.
KJ892568 - Synthetic construct Homo sapiens clone ccsbBroadEn_01962 STK16 gene, encodes complete protein.
KJ897874 - Synthetic construct Homo sapiens clone ccsbBroadEn_07268 STK16 gene, encodes complete protein.
KJ905358 - Synthetic construct Homo sapiens clone ccsbBroadEn_14907 STK16 gene, encodes complete protein.
EU831979 - Synthetic construct Homo sapiens clone HAIB:100067008; DKFZo008G0823 serine/threonine kinase 16 protein (STK16) gene, encodes complete protein.
BT019585 - Homo sapiens serine/threonine kinase 16 mRNA, complete cds.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K9H9, AK310793, MPSK1, NM_001008910, NP_001008910, O75716, PKL12, Q5U0F8, Q96KI2, Q9BUH4, Q9UEN3, Q9UP78, STK16_HUMAN, TSF1
UCSC ID: uc010fwf.3
RefSeq Accession: NM_001008910
Protein: O75716 (aka STK16_HUMAN or STKG_HUMAN)

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: AK310793.1
exon count: 6CDS single in 3' UTR: no RNA size: 1513
ORF size: 843CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1526.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.