Human Gene MAP3K13 (uc010hyf.3)
  Description: Homo sapiens mitogen-activated protein kinase kinase kinase 13 (MAP3K13), transcript variant 2, mRNA.
RefSeq Summary (NM_001242314): The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr3:185,000,729-185,206,882 Size: 206,154 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg19 chr3:185,146,370-185,200,244 Size: 53,875 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:185,000,729-185,206,882)mRNA (may differ from genome)Protein (966 aa)
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-  Comments and Description Text from UniProtKB
  ID: M3K13_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase kinase kinase 13; EC=2.7.11.25; AltName: Full=Leucine zipper-bearing kinase; AltName: Full=Mixed lineage kinase; Short=MLK;
FUNCTION: Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activated by autophosphorylation and homodimerization.
SUBUNIT: Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain. Binds PRDX3. Associates with the IKK complex through the kinase domain.
INTERACTION: Self; NbExp=2; IntAct=EBI-1168480, EBI-1168480;
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas.
PTM: Autophosphorylated on serine and threonine residues.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAI11727.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=BAG59505.1; Type=Erroneous termination; Positions=740; Note=Translated as Tyr;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): MAP3K13
CDC HuGE Published Literature: MAP3K13
Positive Disease Associations: Diabetes Mellitus, Type 2
Related Studies:
  1. Diabetes Mellitus, Type 2
    Laura J Scott et al. Science (New York, N.Y.) 2007, A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants., Science (New York, N.Y.). [PubMed 17463248]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.12 RPKM in Pituitary
Total median expression: 174.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -95.49291-0.328 Picture PostScript Text
3' UTR -1987.296638-0.299 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR017419 - MAP3K12_MAP3K13
IPR000719 - Prot_kinase_cat_dom
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on O43283
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
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 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019901 protein kinase binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding

Biological Process:
GO:0000186 activation of MAPKK activity
GO:0006468 protein phosphorylation
GO:0007254 JNK cascade
GO:0016310 phosphorylation
GO:0046777 protein autophosphorylation
GO:0051092 positive regulation of NF-kappaB transcription factor activity

Cellular Component:
GO:0005737 cytoplasm
GO:0016020 membrane
GO:0008385 IkappaB kinase complex


-  Descriptions from all associated GenBank mRNAs
  AK297646 - Homo sapiens cDNA FLJ58266 complete cds, highly similar to Mitogen-activated protein kinase kinase kinase 13 (EC 2.7.11.25).
AK312714 - Homo sapiens cDNA, FLJ93116, Homo sapiens mitogen-activated protein kinase kinase kinase 13 (MAP3K13), mRNA.
AK302951 - Homo sapiens cDNA FLJ58309 complete cds, highly similar to Mitogen-activated protein kinase kinasekinase 13 (EC 2.7.11.25).
BC031677 - Homo sapiens mitogen-activated protein kinase kinase kinase 13, mRNA (cDNA clone IMAGE:5167124), complete cds.
BC036593 - Homo sapiens cDNA clone IMAGE:4815204.
BC015996 - Homo sapiens, clone IMAGE:4294444, mRNA.
AK296961 - Homo sapiens cDNA FLJ56375 complete cds, highly similar to Mitogen-activated protein kinase kinase kinase 13 (EC 2.7.11.25).
AB001872 - Homo sapiens mRNA for leucine zipper bearing kinase, complete cds.
BC111726 - Homo sapiens mitogen-activated protein kinase kinase kinase 13, mRNA (cDNA clone MGC:133196 IMAGE:40028559), complete cds.
JD565432 - Sequence 546456 from Patent EP1572962.
JD279486 - Sequence 260510 from Patent EP1572962.
Z25428 - H.sapiens protein-serine/threonine kinase gene, complete CDS.
KJ901950 - Synthetic construct Homo sapiens clone ccsbBroadEn_11344 MAP3K13 gene, encodes complete protein.
JD469411 - Sequence 450435 from Patent EP1572962.
JD093728 - Sequence 74752 from Patent EP1572962.
JD103417 - Sequence 84441 from Patent EP1572962.
JD324612 - Sequence 305636 from Patent EP1572962.
JD266235 - Sequence 247259 from Patent EP1572962.
JD350095 - Sequence 331119 from Patent EP1572962.
JD274556 - Sequence 255580 from Patent EP1572962.
JD352637 - Sequence 333661 from Patent EP1572962.
JD220377 - Sequence 201401 from Patent EP1572962.
JD222401 - Sequence 203425 from Patent EP1572962.
AK123148 - Homo sapiens cDNA FLJ41153 fis, clone BRACE2038492.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04010 - MAPK signaling pathway

BioCarta from NCI Cancer Genome Anatomy Project
h_mapkPathway - MAPKinase Signaling Pathway

-  Other Names for This Gene
  Alternate Gene Symbols: B2R6U2, B4DLE3, B4DMV2, B4DZJ4, D3DNU1, LZK, M3K13_HUMAN, NM_001242314, NP_004712, O43283, Q05BY6, Q15450, Q2NKN3
UCSC ID: uc010hyf.3
RefSeq Accession: NM_001242314
Protein: O43283 (aka M3K13_HUMAN)
CCDS: CCDS3270.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001242314.1
exon count: 15CDS single in 3' UTR: no RNA size: 9830
ORF size: 2901CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 5093.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.