Human Gene ADRA1A (uc010lum.1)
  Description: Homo sapiens adrenoceptor alpha 1A (ADRA1A), transcript variant 3, mRNA.
RefSeq Summary (NM_033302): Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr8:26,613,913-26,722,922 Size: 109,010 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr8:26,614,061-26,722,486 Size: 108,426 Coding Exon Count: 2 

Page IndexSequence and LinksPrimersGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:26,613,913-26,722,922)mRNA (may differ from genome)Protein (372 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
HGNCHuman Cortex Gene ExpressionLynxMalacardsMGIOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): ADRA1A
CDC HuGE Published Literature: ADRA1A
Positive Disease Associations: Body Weight , Cholesterol, LDL , hypertension , schizophrenia , schizophrenia; schizoaffective disorder
Related Studies:
  1. Body Weight
    , , . [PubMed 0]
  2. Cholesterol, LDL
    , , . [PubMed 0]
  3. hypertension
    Gu, D. et al. 2006, Association of alpha1A adrenergic receptor gene variants on chromosome 8p21 with human stage 2 hypertension, J Hypertens 2006 24(6) 1057-1064. [PubMed 16685204]
    Our findings suggest that the genetic variations in the ADRA1A gene are significantly associated with essential hypertension, and may play an important role in the development of essential hypertension in this Chinese population.
           more ... click here to view the complete list

-  MalaCards Disease Associations
  MalaCards Gene Search: ADRA1A
Diseases sorted by gene-association score: horner's syndrome (14), ureter cancer (12), prostatic hypertrophy (11), ureterolithiasis (8), prostatitis (7), gilles de la tourette syndrome (5), attention deficit-hyperactivity disorder (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 8.44 RPKM in Liver
Total median expression: 26.52 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -193.51436-0.444 Picture PostScript Text
3' UTR -29.10148-0.197 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P35348-9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
 Gene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Descriptions from all associated GenBank mRNAs
  AY491779 - Homo sapiens alpha 1A adrenoceptor isoform 5a (ADRA1A) mRNA, complete cds, alternatively spliced.
AY491775 - Homo sapiens alpha 1A adrenoceptor isoform 2b (ADRA1A) mRNA, complete cds, alternatively spliced.
AY491776 - Homo sapiens alpha 1A adrenoceptor isoform 2c (ADRA1A) mRNA, complete cds, alternatively spliced.
D32202 - Homo sapiens mRNA for alpha 1C adrenergic receptor isoform 2, complete cds.
E11339 - Human cDNA encoding alpha 1c2 adrenaline receptor.
AY491777 - Homo sapiens alpha 1A adrenoceptor isoform 3b (ADRA1A) mRNA, complete cds, alternatively spliced.
AY491778 - Homo sapiens alpha 1A adrenoceptor isoform 3c (ADRA1A) mRNA, complete cds, alternatively spliced.
D32201 - Homo sapiens mRNA for alpha 1C adrenergic receptor isoform 3, complete cds.
E11340 - Human cDNA encoding alpha 1c3 adrenaline receptor.
JD184859 - Sequence 165883 from Patent EP1572962.
JD044186 - Sequence 25210 from Patent EP1572962.
JD263818 - Sequence 244842 from Patent EP1572962.
JD085472 - Sequence 66496 from Patent EP1572962.
JD423063 - Sequence 404087 from Patent EP1572962.
JD125915 - Sequence 106939 from Patent EP1572962.
AF013261 - Homo sapiens alpha 1A adrenergic receptor isoform 4 mRNA, complete cds.
D25235 - Homo sapiens mRNA for alpha1C adrenergic receptor, complete cds.
E08988 - cDNA encoding human alpha 1c adrenergic receptor.
AK289548 - Homo sapiens cDNA FLJ77251 complete cds, highly similar to Homo sapiens adrenergic, alpha-1A-, receptor (ADRA1A), transcript variant 1, mRNA.
U02569 - Human alpha1C adrenergic receptor mRNA, complete cds.
E08989 - cDNA encoding human alpha 1c adrenergic receptor.
BC095512 - Homo sapiens adrenergic, alpha-1A-, receptor, mRNA (cDNA clone MGC:111726 IMAGE:6971913), complete cds.
L31774 - Human alpha-1C-adrenergic receptor mRNA, complete cds.
AF395806 - Homo sapiens adrenergic receptor alpha-1a (ADRA1A) mRNA, complete cds.
S76001 - alpha 1c-adrenoceptor subtype [human, brain, mRNA Partial, 432 nt].
AY491781 - Homo sapiens alpha 1A adrenoceptor isoform 6 (ADRA1A) mRNA, complete cds, alternatively spliced.
AY491780 - Homo sapiens alpha 1A adrenoceptor isoform 5b (ADRA1A) mRNA, complete cds, alternatively spliced.
AK311558 - Homo sapiens cDNA, FLJ18600.
JD108786 - Sequence 89810 from Patent EP1572962.
JD099863 - Sequence 80887 from Patent EP1572962.
JD525952 - Sequence 506976 from Patent EP1572962.
JD358497 - Sequence 339521 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04020 - Calcium signaling pathway
hsa04080 - Neuroactive ligand-receptor interaction
hsa04270 - Vascular smooth muscle contraction

Reactome (by CSHL, EBI, and GO)

Protein P35348 (Reactome details) participates in the following event(s):

R-HSA-390641 Alpha-1 adrenoceptors bind catecholamines
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-751019 The Ligand:GPCR:G12/13 complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-751027 Liganded G12/13-activating GPCRs bind inactive heterotrimeric G-protein G12/13
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-751029 Liganded G12/13-activating GPCR acts as a GEF for G12/13
R-HSA-390696 Adrenoceptors
R-HSA-416476 G alpha (q) signalling events
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-375280 Amine ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ADRA1C, AY491778, NM_033302, NP_150645, P35348-9
UCSC ID: uc010lum.1
RefSeq Accession: NM_033302
Protein: P35348-9, splice isoform of P35348

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: AY491778.1
exon count: 2CDS single in 3' UTR: no RNA size: 1703
ORF size: 1119CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2047.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.