Human Gene TSPAN32 (uc010qxk.2)
  Description: Homo sapiens tetraspanin 32 (TSPAN32), mRNA.
RefSeq Summary (NM_139022): This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. Sequence Note: A downstream start codon is selected for this RefSeq based on better conservation with homologs. The use of an alternative upstream start codon, which is present in primate species, would increase the protein length from 320 aa to 355 aa. The presence of a predicted signal anchor for the shorter, but not the longer, protein suggests that the downstream start codon may be preferentially used. The shorter protein is described in PMID 11718897.
Transcript (Including UTRs)
   Position: hg19 chr11:2,323,243-2,338,042 Size: 14,800 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr11:2,323,275-2,337,302 Size: 14,028 Coding Exon Count: 7 

Page IndexSequence and LinksPrimersMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:2,323,243-2,338,042)mRNA (may differ from genome)Protein (283 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsGeneNetworkH-INV
HGNCLynxMalacardsMGIPubMedUniProtKB
Wikipedia

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TSPAN32
Diseases sorted by gene-association score: beckwith-wiedemann syndrome (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 8.06 RPKM in Whole Blood
Total median expression: 43.28 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -2.6032-0.081 Picture PostScript Text
3' UTR -345.54740-0.467 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023396 - CD81_extracellular
IPR000301 - Tetraspanin
IPR018499 - Tetraspanin/Peripherin
IPR008952 - Tetraspanin_EC2

SCOP Domains:
48652 - Tetraspanin

ModBase Predicted Comparative 3D Structure on B4DQ90
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Cellular Component:
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK307638 - Homo sapiens cDNA, FLJ97586.
BC016693 - Homo sapiens tetraspanin 32, mRNA (cDNA clone MGC:22455 IMAGE:4071111), complete cds.
AK128812 - Homo sapiens cDNA FLJ46034 fis, clone SPLEN2033996.
JD183199 - Sequence 164223 from Patent EP1572962.
JD103077 - Sequence 84101 from Patent EP1572962.
JD077368 - Sequence 58392 from Patent EP1572962.
JD484908 - Sequence 465932 from Patent EP1572962.
JD255692 - Sequence 236716 from Patent EP1572962.
JD444489 - Sequence 425513 from Patent EP1572962.
JD200577 - Sequence 181601 from Patent EP1572962.
JD392735 - Sequence 373759 from Patent EP1572962.
JD465702 - Sequence 446726 from Patent EP1572962.
JD472949 - Sequence 453973 from Patent EP1572962.
JD445863 - Sequence 426887 from Patent EP1572962.
AY039001 - Homo sapiens tetraspanin (TSSC6) mRNA, complete cds.
AK310116 - Homo sapiens cDNA, FLJ17158.
CU677677 - Synthetic construct Homo sapiens gateway clone IMAGE:100016910 5' read TSPAN32 mRNA.
KJ904643 - Synthetic construct Homo sapiens clone ccsbBroadEn_14037 TSPAN32 gene, encodes complete protein.
AK298692 - Homo sapiens cDNA FLJ52720 complete cds, highly similar to Tetraspanin-32.
AF176070 - Homo sapiens PHEMX (Phemx) mRNA, complete cds, alternatively spliced.
AF125569 - Homo sapiens tumor suppressing STF cDNA 6 (TSSC6) mRNA, complete cds.
AY303780 - Homo sapiens pan-hematopoietic expression protein (PHEMX) mRNA, complete cds; alternatively spliced.
AF176071 - Homo sapiens PHEMX (Phemx) mRNA, partial cds, alternatively spliced.
JD143614 - Sequence 124638 from Patent EP1572962.
JD278766 - Sequence 259790 from Patent EP1572962.
JD475601 - Sequence 456625 from Patent EP1572962.
JD097479 - Sequence 78503 from Patent EP1572962.
JD068804 - Sequence 49828 from Patent EP1572962.
JD514551 - Sequence 495575 from Patent EP1572962.
JD151407 - Sequence 132431 from Patent EP1572962.
JD141023 - Sequence 122047 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AK298692, B4DQ90, B4DQ90_HUMAN
UCSC ID: uc010qxk.2
RefSeq Accession: NM_139022
Protein: B4DQ90

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: AK298692.1
exon count: 7CDS single in 3' UTR: no RNA size: 1450
ORF size: 852CDS single in intron: no Alignment % ID: 99.79
txCdsPredict score: 1493.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.