Description: Homo sapiens polo-like kinase 4 (PLK4), transcript variant 2, mRNA. RefSeq Summary (NM_001190799): This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]. Transcript (Including UTRs) Position: hg19 chr4:128,802,016-128,820,377 Size: 18,362 Total Exon Count: 15 Strand: + Coding Region Position: hg19 chr4:128,802,290-128,819,696 Size: 17,407 Coding Exon Count: 15
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Protein Domain and Structure Information
Pfam Domains: PF00069 - Protein kinase domain PF00659 - POLO box duplicated region PF07714 - Protein tyrosine kinase
ModBase Predicted Comparative 3D Structure on O00444-2
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK314238 - Homo sapiens cDNA, FLJ94982, highly similar to Homo sapiens polo-like kinase 4 (Drosophila) (PLK4), mRNA. BC036023 - Homo sapiens polo-like kinase 4 (Drosophila), mRNA (cDNA clone MGC:33045 IMAGE:5273226), complete cds. AK303399 - Homo sapiens cDNA FLJ50332 complete cds, highly similar to Serine/threonine-protein kinase PLK4 (EC 2.7.11.21). AB006972 - Homo sapiens mRNA for Sak, complete cds. Y13115 - Homo sapiens mRNA for serine/threonine protein kinase SAK. JD480174 - Sequence 461198 from Patent EP1572962. JD196869 - Sequence 177893 from Patent EP1572962. AB741646 - Homo sapiens PLK4 mRNA for polo-like kinase 4, complete cds. DQ894189 - Synthetic construct Homo sapiens clone IMAGE:100008649; FLH168487.01L; RZPDo839H0791D polo-like kinase 4 (Drosophila) (PLK4) gene, encodes complete protein. CU690262 - Synthetic construct Homo sapiens gateway clone IMAGE:100017863 5' read PLK4 mRNA. KJ905400 - Synthetic construct Homo sapiens clone ccsbBroadEn_14973 PLK4 gene, encodes complete protein. AK302858 - Homo sapiens cDNA FLJ50927 complete cds, highly similar to Serine/threonine-protein kinase PLK4 (EC 2.7.11.21). Z25433 - H.sapiens protein-serine/threonine kinase gene, complete CDS. JD130204 - Sequence 111228 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O00444 (Reactome details) participates in the following event(s):
R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-5617833 Cilium Assembly R-HSA-68877 Mitotic Prometaphase R-HSA-69275 G2/M Transition R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-68886 M Phase R-HSA-453274 Mitotic G2-G2/M phases R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle