Human Gene PLK4 (uc011cgs.2)
  Description: Homo sapiens polo-like kinase 4 (PLK4), transcript variant 2, mRNA.
RefSeq Summary (NM_001190799): This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010].
Transcript (Including UTRs)
   Position: hg19 chr4:128,802,016-128,820,377 Size: 18,362 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg19 chr4:128,802,290-128,819,696 Size: 17,407 Coding Exon Count: 15 

Page IndexSequence and LinksPrimersGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:128,802,016-128,820,377)mRNA (may differ from genome)Protein (938 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCLynxMalacardsMGIOMIM
PubMedReactomeTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): PLK4
CDC HuGE Published Literature: PLK4

-  MalaCards Disease Associations
  MalaCards Gene Search: PLK4
Diseases sorted by gene-association score: microcephaly and chorioretinopathy, autosomal recessive, 2* (1019), autosomal recessive chorioretinopathy-microcephaly syndrome* (202), seckel syndrome* (135), sertoli cell-only syndrome (8), microcephaly (3)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.26 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 46.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -116.80274-0.426 Picture PostScript Text
3' UTR -127.59681-0.187 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF00069 - Protein kinase domain
PF00659 - POLO box duplicated region
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)
82615 - Polo-box domain

ModBase Predicted Comparative 3D Structure on O00444-2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Descriptions from all associated GenBank mRNAs
  AK314238 - Homo sapiens cDNA, FLJ94982, highly similar to Homo sapiens polo-like kinase 4 (Drosophila) (PLK4), mRNA.
BC036023 - Homo sapiens polo-like kinase 4 (Drosophila), mRNA (cDNA clone MGC:33045 IMAGE:5273226), complete cds.
AK303399 - Homo sapiens cDNA FLJ50332 complete cds, highly similar to Serine/threonine-protein kinase PLK4 (EC 2.7.11.21).
AB006972 - Homo sapiens mRNA for Sak, complete cds.
Y13115 - Homo sapiens mRNA for serine/threonine protein kinase SAK.
JD480174 - Sequence 461198 from Patent EP1572962.
JD196869 - Sequence 177893 from Patent EP1572962.
AB741646 - Homo sapiens PLK4 mRNA for polo-like kinase 4, complete cds.
DQ894189 - Synthetic construct Homo sapiens clone IMAGE:100008649; FLH168487.01L; RZPDo839H0791D polo-like kinase 4 (Drosophila) (PLK4) gene, encodes complete protein.
CU690262 - Synthetic construct Homo sapiens gateway clone IMAGE:100017863 5' read PLK4 mRNA.
KJ905400 - Synthetic construct Homo sapiens clone ccsbBroadEn_14973 PLK4 gene, encodes complete protein.
AK302858 - Homo sapiens cDNA FLJ50927 complete cds, highly similar to Serine/threonine-protein kinase PLK4 (EC 2.7.11.21).
Z25433 - H.sapiens protein-serine/threonine kinase gene, complete CDS.
JD130204 - Sequence 111228 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O00444 (Reactome details) participates in the following event(s):

R-HSA-380272 Plk1-mediated phosphorylation of Nlp
R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome
R-HSA-380294 Loss of C-Nap-1 from centrosomes
R-HSA-380311 Recruitment of Plk1 to centrosomes
R-HSA-380455 Recruitment of CDK11p58 to the centrosomes
R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome
R-HSA-5626220 C2CD3 binds the mother centriole
R-HSA-380508 Translocation of NuMA to the centrosomes
R-HSA-2574845 AJUBA binds centrosome-associated AURKA
R-HSA-8853405 TPX2 binds AURKA at centrosomes
R-HSA-3000319 BORA binds PLK1 and AURKA
R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation
R-HSA-3000310 AURKA phosphorylates PLK1
R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole
R-HSA-5626681 Recruitment of transition zone proteins
R-HSA-5626227 CP110 and CEP97 dissociate from the centriole
R-HSA-380316 Association of NuMA with microtubules
R-HSA-8853419 TPX2 promotes AURKA autophosphorylation
R-HSA-5626228 The distal appendage proteins recruit TTBK2
R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body
R-HSA-5626699 MARK4 binds ODF2 in the centriole
R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-8854518 AURKA Activation by TPX2
R-HSA-380287 Centrosome maturation
R-HSA-5617833 Cilium Assembly
R-HSA-68877 Mitotic Prometaphase
R-HSA-69275 G2/M Transition
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-68886 M Phase
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: NM_001190799, NP_001177728, O00444-2, SAK, STK18
UCSC ID: uc011cgs.2
RefSeq Accession: NM_001190799
Protein: O00444-2, splice isoform of O00444 CCDS: CCDS54804.1, CCDS3735.1, CCDS54803.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001190799.1
exon count: 15CDS single in 3' UTR: no RNA size: 3774
ORF size: 2817CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 5649.00frame shift in genome: no % Coverage: 99.95
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.