Human Gene MED6 (ENST00000256379.10) from GENCODE V44
  Description: Homo sapiens mediator complex subunit 6 (MED6), transcript variant 2, mRNA. (from RefSeq NM_005466)
Gencode Transcript: ENST00000256379.10
Gencode Gene: ENSG00000133997.12
Transcript (Including UTRs)
   Position: hg38 chr14:70,583,221-70,600,655 Size: 17,435 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg38 chr14:70,584,813-70,600,637 Size: 15,825 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr14:70,583,221-70,600,655)mRNA (may differ from genome)Protein (246 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 6; AltName: Full=Activator-recruited cofactor 33 kDa component; Short=ARC33; AltName: Full=Mediator complex subunit 6; Short=hMed6; AltName: Full=Renal carcinoma antigen NY-REN-28;
FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CTNNB1 and GLI3.
SIMILARITY: Belongs to the Mediator complex subunit 6 family.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.34 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 324.52 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR 0.00180.000 Picture PostScript Text
3' UTR -425.801592-0.267 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007018 - Mediator_Med6
IPR016820 - Mediator_Med6_met/pln

Pfam Domains:
PF04934 - MED6 mediator sub complex component

ModBase Predicted Comparative 3D Structure on O75586
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001104 RNA polymerase II transcription cofactor activity
GO:0001128 RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0008134 transcription factor binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051123 RNA polymerase II transcriptional preinitiation complex assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016020 membrane
GO:0016592 mediator complex
GO:0070847 core mediator complex

-  Descriptions from all associated GenBank mRNAs
  AK222587 - Homo sapiens mRNA for mediator of RNA polymerase II transcription, subunit 6 homolog, clone: CAS02217.
LP895303 - Sequence 167 from Patent EP3253886.
AK300460 - Homo sapiens cDNA FLJ52756 complete cds, highly similar to RNA polymerase transcriptional regulation mediator, subunit 6 homolog.
AF155104 - Homo sapiens NY-REN-28 antigen mRNA, partial cds.
AK223346 - Homo sapiens mRNA for mediator of RNA polymerase II transcription, subunit 6 homolog, clone: TST01693.
AK290647 - Homo sapiens cDNA FLJ76264 complete cds, highly similar to Homo sapiens mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) (MED6), mRNA.
BC004106 - Homo sapiens mediator complex subunit 6, mRNA (cDNA clone MGC:10604 IMAGE:3936675), complete cds.
LF384865 - JP 2014500723-A/192368: Polycomb-Associated Non-Coding RNAs.
MA620442 - JP 2018138019-A/192368: Polycomb-Associated Non-Coding RNAs.
JD472301 - Sequence 453325 from Patent EP1572962.
JD165418 - Sequence 146442 from Patent EP1572962.
JD481548 - Sequence 462572 from Patent EP1572962.
AK304361 - Homo sapiens cDNA FLJ59149 complete cds, highly similar to RNA polymerase transcriptional regulation mediator, subunit 6 homolog.
AF074723 - Homo sapiens RNA polymerase transcriptional regulation mediator (MED6) mRNA, complete cds.
JD533666 - Sequence 514690 from Patent EP1572962.
JD291717 - Sequence 272741 from Patent EP1572962.
JD421859 - Sequence 402883 from Patent EP1572962.
KJ892891 - Synthetic construct Homo sapiens clone ccsbBroadEn_02285 MED6 gene, encodes complete protein.
BT006831 - Homo sapiens mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) mRNA, complete cds.
AB463868 - Synthetic construct DNA, clone: pF1KB7518, Homo sapiens MED6 gene for mediator complex subunit 6, without stop codon, in Flexi system.
CR541697 - Homo sapiens full open reading frame cDNA clone RZPDo834E0428D for gene MED6, mediator of RNA polymerase II transcription, subunit 6 homolog (yeast); complete cds, without stopcodon.
LF329896 - JP 2014500723-A/137399: Polycomb-Associated Non-Coding RNAs.
MA565473 - JP 2018138019-A/137399: Polycomb-Associated Non-Coding RNAs.
U78082 - Human RNA polymerase transcriptional regulation mediator (h-MED6) mRNA, complete cds.
AK300417 - Homo sapiens cDNA FLJ57086 complete cds, highly similar to RNA polymerase transcriptional regulation mediator, subunit 6 homolog.
LF329900 - JP 2014500723-A/137403: Polycomb-Associated Non-Coding RNAs.
MA565477 - JP 2018138019-A/137403: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O75586 (Reactome details) participates in the following event(s):

R-HSA-212352 Formation of ARC coactivator complex
R-HSA-212380 Formation of TRAP coactivator complex
R-HSA-212432 Formation of DRIP coactivator complex
R-HSA-381309 PPARG:RXRA heterodimer binds to fatty acid-like ligands
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-1266738 Developmental Biology
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: ARC33, ENST00000256379.1, ENST00000256379.2, ENST00000256379.3, ENST00000256379.4, ENST00000256379.5, ENST00000256379.6, ENST00000256379.7, ENST00000256379.8, ENST00000256379.9, MED6_HUMAN, NM_005466, O15401, O75586, Q53FE3, Q53HJ3, Q6FHQ4, Q9BTH1, Q9UHL1, uc001xmf.1, uc001xmf.2, uc001xmf.3, uc001xmf.4, uc001xmf.5, uc001xmf.6
UCSC ID: ENST00000256379.10
RefSeq Accession: NM_005466
Protein: O75586 (aka MED6_HUMAN)
CCDS: CCDS9805.1, CCDS61484.1, CCDS73649.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.