Human Gene ZWINT (ENST00000373944.8) from GENCODE V44
  Description: Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA. (from RefSeq NM_007057)
RefSeq Summary (NM_007057): This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000373944.8
Gencode Gene: ENSG00000122952.17
Transcript (Including UTRs)
   Position: hg38 chr10:56,357,227-56,361,259 Size: 4,033 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg38 chr10:56,358,418-56,361,236 Size: 2,819 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr10:56,357,227-56,361,259)mRNA (may differ from genome)Protein (277 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=ZW10 interactor; AltName: Full=ZW10-interacting protein 1; Short=Zwint-1;
FUNCTION: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
SUBUNIT: Interacts with ZW10 and MIS12. Interacts with the NDC80 subunit of the NDC80 complex specifically during mitosis. Also interacts with CASC5, CETN3, DSN1 and PMF1.
INTERACTION: O14777:NDC80; NbExp=11; IntAct=EBI-1001132, EBI-715849; O43264:ZW10; NbExp=6; IntAct=EBI-1001132, EBI-1001217;
SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 44.61 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 153.18 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -4.2023-0.183 Picture PostScript Text
3' UTR -230.301000-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  ModBase Predicted Comparative 3D Structure on O95229
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0047485 protein N-terminus binding

Biological Process:
GO:0000070 mitotic sister chromatid segregation
GO:0007049 cell cycle
GO:0007093 mitotic cell cycle checkpoint
GO:0051301 cell division
GO:0051649 establishment of localization in cell

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016604 nuclear body
GO:0030425 dendrite
GO:0043005 neuron projection
GO:0043231 intracellular membrane-bounded organelle

-  Descriptions from all associated GenBank mRNAs
  AK093808 - Homo sapiens cDNA FLJ36489 fis, clone THYMU2018126, highly similar to ZW10 interactor.
LF384646 - JP 2014500723-A/192149: Polycomb-Associated Non-Coding RNAs.
MA620223 - JP 2018138019-A/192149: Polycomb-Associated Non-Coding RNAs.
AF067656 - Homo sapiens ZW10 interactor Zwint mRNA, complete cds.
AK225509 - Homo sapiens mRNA for ZW10 interactor variant, clone: KAT01661.
BC020979 - Homo sapiens ZW10 interactor, mRNA (cDNA clone MGC:9699 IMAGE:3848873), complete cds.
BC110399 - Homo sapiens ZW10 interactor, mRNA (cDNA clone MGC:117174 IMAGE:5554405), complete cds.
AK298148 - Homo sapiens cDNA FLJ55119 complete cds, highly similar to Homo sapiens ZW10 interactor (ZWINT), transcript variant 3, mRNA.
AK293416 - Homo sapiens cDNA FLJ60420 complete cds, highly similar to ZW10 interactor.
JF432678 - Synthetic construct Homo sapiens clone IMAGE:100073916 ZW10 interactor (ZWINT) gene, encodes complete protein.
KJ898363 - Synthetic construct Homo sapiens clone ccsbBroadEn_07757 ZWINT gene, encodes complete protein.
BC016357 - Homo sapiens ZW10 interactor, mRNA (cDNA clone IMAGE:4094672), partial cds.
BC000411 - Homo sapiens, clone IMAGE:2961008, mRNA, partial cds.
JD496267 - Sequence 477291 from Patent EP1572962.
JD553938 - Sequence 534962 from Patent EP1572962.
JD247657 - Sequence 228681 from Patent EP1572962.
JD563538 - Sequence 544562 from Patent EP1572962.
JD531105 - Sequence 512129 from Patent EP1572962.
JD223684 - Sequence 204708 from Patent EP1572962.
JD188942 - Sequence 169966 from Patent EP1572962.
JD334250 - Sequence 315274 from Patent EP1572962.
JD524930 - Sequence 505954 from Patent EP1572962.
JD187908 - Sequence 168932 from Patent EP1572962.
JD471252 - Sequence 452276 from Patent EP1572962.
JD555842 - Sequence 536866 from Patent EP1572962.
JD400579 - Sequence 381603 from Patent EP1572962.
LF322790 - JP 2014500723-A/130293: Polycomb-Associated Non-Coding RNAs.
MA558367 - JP 2018138019-A/130293: Polycomb-Associated Non-Coding RNAs.
LF322789 - JP 2014500723-A/130292: Polycomb-Associated Non-Coding RNAs.
MA558366 - JP 2018138019-A/130292: Polycomb-Associated Non-Coding RNAs.
LF359976 - JP 2014500723-A/167479: Polycomb-Associated Non-Coding RNAs.
MA595553 - JP 2018138019-A/167479: Polycomb-Associated Non-Coding RNAs.
LF322788 - JP 2014500723-A/130291: Polycomb-Associated Non-Coding RNAs.
MA558365 - JP 2018138019-A/130291: Polycomb-Associated Non-Coding RNAs.
LF359975 - JP 2014500723-A/167478: Polycomb-Associated Non-Coding RNAs.
MA595552 - JP 2018138019-A/167478: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O95229 (Reactome details) participates in the following event(s):

R-HSA-141409 Mad1 binds kinetochore
R-HSA-375302 Kinetochore capture of astral microtubules
R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores
R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2
R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex
R-HSA-141439 Release of activated MAD2 from kinetochores
R-HSA-2467811 Separation of sister chromatids
R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin
R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores
R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1
R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin
R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres
R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-68877 Mitotic Prometaphase
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-68886 M Phase
R-HSA-195258 RHO GTPase Effectors
R-HSA-68882 Mitotic Anaphase
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-194315 Signaling by Rho GTPases
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-1640170 Cell Cycle
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000373944.1, ENST00000373944.2, ENST00000373944.3, ENST00000373944.4, ENST00000373944.5, ENST00000373944.6, ENST00000373944.7, NM_007057, O95229, Q0D2I3, Q9BWD0, uc001jjx.1, uc001jjx.2, uc001jjx.3, ZWINT_HUMAN
UCSC ID: ENST00000373944.8
RefSeq Accession: NM_007057
Protein: O95229 (aka ZWINT_HUMAN or ZWIN_HUMAN)
CCDS: CCDS7249.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.