Human Gene RGS4 (ENST00000421743.6) from GENCODE V44
  Description: Homo sapiens regulator of G protein signaling 4 (RGS4), transcript variant 1, mRNA. (from RefSeq NM_001102445)
RefSeq Summary (NM_001102445): Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000421743.6
Gencode Gene: ENSG00000117152.14
Transcript (Including UTRs)
   Position: hg38 chr1:163,068,775-163,076,802 Size: 8,028 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr1:163,068,935-163,074,560 Size: 5,626 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:163,068,775-163,076,802)mRNA (may differ from genome)Protein (302 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGencode
GeneCardsHGNCHPRDLynxMalacardsMGI
neXtProtOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RGS4_HUMAN
DESCRIPTION: RecName: Full=Regulator of G-protein signaling 4; Short=RGP4; Short=RGS4;
FUNCTION: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
TISSUE SPECIFICITY: Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum.
PTM: Palmitoylated on Cys-2 and/or Cys-12.
PTM: Phosphorylated by cyclic GMP-dependent protein kinase (By similarity).
DISEASE: Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
SIMILARITY: Contains 1 RGS domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: RGS4
Diseases sorted by gene-association score: schizophrenia 9 (13), schizophrenia (10), bipolar disorder (9), psychotic disorder (8), schizoaffective disorder (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 94.23 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 316.58 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -36.20160-0.226 Picture PostScript Text
3' UTR -618.702242-0.276 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000342 - Regulat_G_prot_signal
IPR024066 - Regulat_G_prot_signal_dom1
IPR016137 - Regulat_G_prot_signal_superfam

Pfam Domains:
PF00615 - Regulator of G protein signaling domain

ModBase Predicted Comparative 3D Structure on P49798
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001965 G-protein alpha-subunit binding
GO:0005096 GTPase activator activity
GO:0005516 calmodulin binding

Biological Process:
GO:0000188 inactivation of MAPK activity
GO:0007186 G-protein coupled receptor signaling pathway
GO:0008277 regulation of G-protein coupled receptor protein signaling pathway
GO:0009968 negative regulation of signal transduction
GO:0043547 positive regulation of GTPase activity
GO:0045744 negative regulation of G-protein coupled receptor protein signaling pathway

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  DQ346664 - Homo sapiens regulator of G-protein signaling 4 isoform C (RGS4) mRNA, complete cds, alternatively spliced.
AK295540 - Homo sapiens cDNA FLJ60558 complete cds, highly similar to Regulator of G-protein signaling 4.
DQ346663 - Homo sapiens regulator of G-protein signaling 4 transcript isoform B (RGS4) mRNA, complete cds, alternatively spliced.
AK300832 - Homo sapiens cDNA FLJ57797 complete cds, moderately similar to Regulator of G-protein signaling 4.
AK093959 - Homo sapiens cDNA FLJ36640 fis, clone TRACH2019151, moderately similar to REGULATOR OF G-PROTEIN SIGNALING 4.
DQ346662 - Homo sapiens regulator of G-protein signaling 4 transcript isoform A (RGS4) mRNA, complete cds, alternatively spliced.
AK312250 - Homo sapiens cDNA, FLJ92545, Homo sapiens regulator of G-protein signalling 4 (RGS4), mRNA.
AK096204 - Homo sapiens cDNA FLJ38885 fis, clone MESAN2017417, moderately similar to REGULATOR OF G-PROTEIN SIGNALING 4.
BC051869 - Homo sapiens regulator of G-protein signaling 4, mRNA (cDNA clone MGC:60244 IMAGE:5786695), complete cds.
U27768 - Homo sapiens RGP4 mRNA, complete cds.
BC000737 - Homo sapiens regulator of G-protein signaling 4, mRNA (cDNA clone MGC:2124 IMAGE:3510260), complete cds.
JD313157 - Sequence 294181 from Patent EP1572962.
AB528687 - Synthetic construct DNA, clone: pF1KB6913, Homo sapiens RGS4 gene for regulator of G-protein signaling 4, without stop codon, in Flexi system.
BT007025 - Homo sapiens regulator of G-protein signalling 4 mRNA, complete cds.
KJ892002 - Synthetic construct Homo sapiens clone ccsbBroadEn_01396 RGS4 gene, encodes complete protein.
AF493928 - Homo sapiens regulator of G protein signalling 4 (RGS4) mRNA, complete cds.
AB209019 - Homo sapiens mRNA for regulator of G-protein signaling 4 variant protein.
EF054877 - Homo sapiens regulator of G-protein signaling 4 isoform 5 (RGS4) mRNA, complete cds.
AK296240 - Homo sapiens cDNA FLJ58002 complete cds, highly similar to Regulator of G-protein signaling 4.
AL137433 - Homo sapiens mRNA; cDNA DKFZp761F1924 (from clone DKFZp761F1924).

-  Other Names for This Gene
  Alternate Gene Symbols: A7XA56, A7XA58, A7XA59, A7YVV7, B1APZ3, ENST00000421743.1, ENST00000421743.2, ENST00000421743.3, ENST00000421743.4, ENST00000421743.5, NM_001102445, P49798, RGS4_HUMAN, uc001gcl.1, uc001gcl.2, uc001gcl.3, uc001gcl.4, uc001gcl.5
UCSC ID: ENST00000421743.6
RefSeq Accession: NM_001102445
Protein: P49798 (aka RGS4_HUMAN)
CCDS: CCDS44270.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.