Human Gene PLEKHS1 (ENST00000619563.5) from GENCODE V44
  Description: Homo sapiens pleckstrin homology domain containing S1 (PLEKHS1), transcript variant 3, mRNA. (from RefSeq NM_001193435)
Gencode Transcript: ENST00000619563.5
Gencode Gene: ENSG00000148735.16
Transcript (Including UTRs)
   Position: hg38 chr10:113,751,682-113,781,237 Size: 29,556 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg38 chr10:113,767,385-113,780,781 Size: 13,397 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:113,751,682-113,781,237)mRNA (may differ from genome)Protein (366 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHGNC
HPRDLynxMalacardsMGIneXtProtPubMed
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PKHS1_HUMAN
DESCRIPTION: RecName: Full=Pleckstrin homology domain-containing family S member 1; Short=PH domain-containing family S member 1; AltName: Full=Epididymis luminal protein 185; Short=hEL185;
SIMILARITY: Contains 1 PH domain.
SEQUENCE CAUTION: Sequence=AAH36365.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB15687.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAI12642.1; Type=Erroneous gene model prediction; Sequence=CAI12643.1; Type=Erroneous gene model prediction; Sequence=CAI12644.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.61 RPKM in Minor Salivary Gland
Total median expression: 47.26 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -73.80267-0.276 Picture PostScript Text
3' UTR -151.40456-0.332 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology

ModBase Predicted Comparative 3D Structure on Q5SXH7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding


-  Descriptions from all associated GenBank mRNAs
  AK128811 - Homo sapiens cDNA FLJ46008 fis, clone SMINT2017964.
AK292867 - Homo sapiens cDNA FLJ77649 complete cds.
AK126354 - Homo sapiens cDNA FLJ44383 fis, clone TRACH3036207.
AB528876 - Synthetic construct DNA, clone: pF1KE0610, Homo sapiens C10orf81 gene for chromosome 10 open reading frame 81, without stop codon, in Flexi system.
BC037870 - Homo sapiens chromosome 10 open reading frame 81, mRNA (cDNA clone MGC:43838 IMAGE:5273287), complete cds.
FJ236306 - Homo sapiens testis secretory sperm binding protein Li 244mP (HTL-S-244mP) mRNA, complete cds.
GQ900988 - Homo sapiens clone HEL-T-100 epididymis secretory sperm binding protein mRNA, complete cds.
BC036365 - Homo sapiens chromosome 10 open reading frame 81, mRNA (cDNA clone IMAGE:4825959), partial cds.
KJ903254 - Synthetic construct Homo sapiens clone ccsbBroadEn_12648 C10orf81 gene, encodes complete protein.
CU690334 - Synthetic construct Homo sapiens gateway clone IMAGE:100018719 5' read C10orf81 mRNA.
JD309970 - Sequence 290994 from Patent EP1572962.
AK027190 - Homo sapiens cDNA: FLJ23537 fis, clone LNG07690.
JD268097 - Sequence 249121 from Patent EP1572962.
AK296132 - Homo sapiens cDNA FLJ51608 complete cds.
JD190207 - Sequence 171231 from Patent EP1572962.
JD044419 - Sequence 25443 from Patent EP1572962.
JD447482 - Sequence 428506 from Patent EP1572962.
JD204188 - Sequence 185212 from Patent EP1572962.
JD161198 - Sequence 142222 from Patent EP1572962.
JD117683 - Sequence 98707 from Patent EP1572962.
JD392185 - Sequence 373209 from Patent EP1572962.
JD356113 - Sequence 337137 from Patent EP1572962.
JD278932 - Sequence 259956 from Patent EP1572962.
JD438491 - Sequence 419515 from Patent EP1572962.
JD438492 - Sequence 419516 from Patent EP1572962.
JD530443 - Sequence 511467 from Patent EP1572962.
JD515290 - Sequence 496314 from Patent EP1572962.
JD322070 - Sequence 303094 from Patent EP1572962.
JD322069 - Sequence 303093 from Patent EP1572962.
JD322075 - Sequence 303099 from Patent EP1572962.
JD322079 - Sequence 303103 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8KA02, B3KX00, B6EDE1, C10orf81, ENST00000619563.1, ENST00000619563.2, ENST00000619563.3, ENST00000619563.4, NM_001193435, PKHS1_HUMAN, Q5SXH7, Q5SXH8, Q5SXI0, Q6ZQR5, Q8NE42, Q9H5D9, uc001las.1, uc001las.2, uc001las.3, uc001las.4
UCSC ID: ENST00000619563.5
RefSeq Accession: NM_001193435
Protein: Q5SXH7 (aka PKHS1_HUMAN)
CCDS: CCDS53581.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.