Human Gene STK33 (ENST00000396672.5) from GENCODE V44
  Description: Homo sapiens serine/threonine kinase 33 (STK33), transcript variant 4, mRNA. (from RefSeq NM_001289061)
Gencode Transcript: ENST00000396672.5
Gencode Gene: ENSG00000130413.16
Transcript (Including UTRs)
   Position: hg38 chr11:8,391,871-8,593,956 Size: 202,086 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg38 chr11:8,392,510-8,474,905 Size: 82,396 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:8,391,871-8,593,956)mRNA (may differ from genome)Protein (514 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: STK33_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase 33; EC=2.7.11.1;
FUNCTION: Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBUNIT: Interacts with VIME (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region (By similarity).
TISSUE SPECIFICITY: Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
PTM: Autophosphorylated (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 20.36 RPKM in Testis
Total median expression: 112.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -115.97374-0.310 Picture PostScript Text
3' UTR -164.10639-0.257 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020636 - Ca/CaM-dep_Ca-dep_prot_Kinase
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain

ModBase Predicted Comparative 3D Structure on Q9BYT3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0035556 intracellular signal transduction
GO:0046777 protein autophosphorylation

Cellular Component:
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK092720 - Homo sapiens cDNA FLJ35401 fis, clone SKNSH2005792, highly similar to Serine/threonine-protein kinase 33 (EC2.7.11.1).
AX747689 - Sequence 1214 from Patent EP1308459.
AK093251 - Homo sapiens cDNA FLJ35932 fis, clone TESTI2011286, weakly similar to MYOSIN LIGHT CHAIN KINASE (EC 2.7.1.117).
AX748014 - Sequence 1539 from Patent EP1308459.
BC031231 - Homo sapiens serine/threonine kinase 33, mRNA (cDNA clone MGC:39551 IMAGE:5296502), complete cds.
AK293193 - Homo sapiens cDNA FLJ58445 complete cds, highly similar to Serine/threonine-protein kinase 33 (EC 2.7.11.1).
AK310754 - Homo sapiens cDNA, FLJ17796.
AJ303380 - Homo sapiens mRNA for serine/threonine kinase 33 (STK33 gene).
AK122808 - Homo sapiens cDNA FLJ16397 fis, clone TRACH3000043, highly similar to Homo sapiens mRNA for serine/threonine kinase 33.
AL833011 - Homo sapiens mRNA; cDNA DKFZp666M234 (from clone DKFZp666M234).
JD429782 - Sequence 410806 from Patent EP1572962.
JD292058 - Sequence 273082 from Patent EP1572962.
JD167331 - Sequence 148355 from Patent EP1572962.
JD539709 - Sequence 520733 from Patent EP1572962.
DQ891517 - Synthetic construct clone IMAGE:100004147; FLH177653.01X; RZPDo839C07126D serine/threonine kinase 33 (STK33) gene, encodes complete protein.
KJ903124 - Synthetic construct Homo sapiens clone ccsbBroadEn_12518 STK33 gene, encodes complete protein.
KJ905531 - Synthetic construct Homo sapiens clone ccsbBroadEn_15143 STK33 gene, encodes complete protein.
EU176614 - Synthetic construct Homo sapiens clone IMAGE:100011407; FLH177652.01L; RZPDo839E05254D serine/threonine kinase 33 (STK33) gene, encodes complete protein.
AB528005 - Synthetic construct DNA, clone: pF1KE0778, Homo sapiens STK33 gene for serine/threonine kinase 33, without stop codon, in Flexi system.
CU689122 - Synthetic construct Homo sapiens gateway clone IMAGE:100019746 5' read STK33 mRNA.
JD305490 - Sequence 286514 from Patent EP1572962.
JD501482 - Sequence 482506 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000396672.1, ENST00000396672.2, ENST00000396672.3, ENST00000396672.4, NM_001289061, Q658S6, Q8NEF5, Q9BYT3, STK33_HUMAN, uc001mgk.1, uc001mgk.2, uc001mgk.3
UCSC ID: ENST00000396672.5
RefSeq Accession: NM_001289061
Protein: Q9BYT3 (aka STK33_HUMAN)
CCDS: CCDS7789.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.