ID:CUL5_HUMAN DESCRIPTION: RecName: Full=Cullin-5; Short=CUL-5; AltName: Full=Vasopressin-activated calcium-mobilizing receptor 1; Short=VACM-1; FUNCTION: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL5, Elongin BC (TCEB1 and TCEB2), RBX2 and a variable SOCS box domain- containing protein as substrate-specific recognition component. Component of the probable ECS(LRRC41) complex with the substrate recognition component LRRC41. Component of the probable ECS(SOCS1) complex with the substrate recognition component SOCS1. Component of the probable ECS(WSB1) complex with the substrate recognition subunit WSB1. Component of the probable ECS(SOCS3) complex with the substrate recognition component SOCS3. Component of the probable ECS(SPSB1) complex with the substrate recognition component SPSB1. Component of the probable ECS(SPSB2) complex with the substrate recognition component SPSB2. Component of the probable ECS(SPSB4) complex with the substrate recognition component SPSB4. Component of the probable ECS(RAB40C) complex with the substrate recognition subunit RAB40C. May also form complexes containing CUL5, elongin BC complex (TCEB1 and TCEB2), RBX1 and TCEB3. May also form complexes containing CUL5, Elongin BC (TCEB1 and TCEB2), RBX1 and VHL. The substrate recognition component can also be a viral protein such as HIV Vif, or human adenovirus 5 E1B large T-antigen and E4-orf6. Interacts with RNF7/RBX2, LRRC41, SOCS3, SPSB1, SPSB2, SPSB4 and RAB40C. Interacts with ASB1, ASB2, ASB6, ASB7 and ASB12. INTERACTION: P62877:RBX1; NbExp=3; IntAct=EBI-1057139, EBI-398523; P12504:vif (xeno); NbExp=5; IntAct=EBI-1057139, EBI-779991; PTM: Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). SIMILARITY: Belongs to the cullin family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q93034
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK125294 - Homo sapiens cDNA FLJ43304 fis, clone NT2NE2005688, highly similar to Vasopressin-activated calcium-mobilizing receptor. AK292575 - Homo sapiens cDNA FLJ75330 complete cds, highly similar to Homo sapiens cullin 5, mRNA. BC063306 - Homo sapiens cullin 5, mRNA (cDNA clone MGC:71728 IMAGE:30331132), complete cds. X81882 - H.sapiens mRNA for for vasopressin activated calcium mobilizing receptor-like protein. BC017838 - Homo sapiens cDNA clone IMAGE:4666355, containing frame-shift errors. CU687672 - Synthetic construct Homo sapiens gateway clone IMAGE:100020944 5' read CUL5 mRNA. KJ897804 - Synthetic construct Homo sapiens clone ccsbBroadEn_07198 CUL5 gene, encodes complete protein. AF327710 - Homo sapiens cullin 5 mRNA, complete cds. AF017061 - Homo sapiens vasopressin-activated calcium mobilizing putative receptor protein (VACM-1) mRNA, complete cds. JD437476 - Sequence 418500 from Patent EP1572962. JD450933 - Sequence 431957 from Patent EP1572962. JD313404 - Sequence 294428 from Patent EP1572962. JD202764 - Sequence 183788 from Patent EP1572962. BC037203 - Homo sapiens cDNA clone IMAGE:4820631. JD491470 - Sequence 472494 from Patent EP1572962. JD114530 - Sequence 95554 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04120 - Ubiquitin mediated proteolysis
Reactome (by CSHL, EBI, and GO)
Protein Q93034 (Reactome details) participates in the following event(s):
R-HSA-180555 Association of APOBEC3G:Vif with the Cul5-SCF complex R-HSA-1918095 CUL5 mediates ubiquitination of ERBB2 R-HSA-180540 Multi-ubiquitination of APOBEC3G R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-983147 Release of E3 from polyubiquitinated substrate R-HSA-8952039 NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-8952044 AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes R-HSA-180585 Vif-mediated degradation of APOBEC3G R-HSA-8863795 Downregulation of ERBB2 signaling R-HSA-162909 Host Interactions of HIV factors R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-8951664 Neddylation R-HSA-1227986 Signaling by ERBB2 R-HSA-162906 HIV Infection R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-597592 Post-translational protein modification R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-5663205 Infectious disease R-HSA-1280218 Adaptive Immune System R-HSA-392499 Metabolism of proteins R-HSA-162582 Signal Transduction R-HSA-1643685 Disease R-HSA-168256 Immune System