Human Gene CUL5 (ENST00000393094.7) from GENCODE V44
  Description: Homo sapiens cullin 5 (CUL5), mRNA. (from RefSeq NM_003478)
Gencode Transcript: ENST00000393094.7
Gencode Gene: ENSG00000166266.14
Transcript (Including UTRs)
   Position: hg38 chr11:108,008,898-108,107,761 Size: 98,864 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg38 chr11:108,009,349-108,104,384 Size: 95,036 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:108,008,898-108,107,761)mRNA (may differ from genome)Protein (780 aa)
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OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CUL5_HUMAN
DESCRIPTION: RecName: Full=Cullin-5; Short=CUL-5; AltName: Full=Vasopressin-activated calcium-mobilizing receptor 1; Short=VACM-1;
FUNCTION: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL5, Elongin BC (TCEB1 and TCEB2), RBX2 and a variable SOCS box domain- containing protein as substrate-specific recognition component. Component of the probable ECS(LRRC41) complex with the substrate recognition component LRRC41. Component of the probable ECS(SOCS1) complex with the substrate recognition component SOCS1. Component of the probable ECS(WSB1) complex with the substrate recognition subunit WSB1. Component of the probable ECS(SOCS3) complex with the substrate recognition component SOCS3. Component of the probable ECS(SPSB1) complex with the substrate recognition component SPSB1. Component of the probable ECS(SPSB2) complex with the substrate recognition component SPSB2. Component of the probable ECS(SPSB4) complex with the substrate recognition component SPSB4. Component of the probable ECS(RAB40C) complex with the substrate recognition subunit RAB40C. May also form complexes containing CUL5, elongin BC complex (TCEB1 and TCEB2), RBX1 and TCEB3. May also form complexes containing CUL5, Elongin BC (TCEB1 and TCEB2), RBX1 and VHL. The substrate recognition component can also be a viral protein such as HIV Vif, or human adenovirus 5 E1B large T-antigen and E4-orf6. Interacts with RNF7/RBX2, LRRC41, SOCS3, SPSB1, SPSB2, SPSB4 and RAB40C. Interacts with ASB1, ASB2, ASB6, ASB7 and ASB12.
INTERACTION: P62877:RBX1; NbExp=3; IntAct=EBI-1057139, EBI-398523; P12504:vif (xeno); NbExp=5; IntAct=EBI-1057139, EBI-779991;
PTM: Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).
SIMILARITY: Belongs to the cullin family.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.02 RPKM in Muscle - Skeletal
Total median expression: 294.00 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -214.70451-0.476 Picture PostScript Text
3' UTR -779.403377-0.231 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016157 - Cullin_CS
IPR016158 - Cullin_homology
IPR001373 - Cullin_N
IPR019559 - Cullin_neddylation_domain
IPR016159 - Cullin_repeat-like_dom
IPR011991 - WHTH_trsnscrt_rep_DNA-bd

Pfam Domains:
PF00888 - Cullin family
PF10557 - Cullin protein neddylation domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3DPL - X-ray 3DQV - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q93034
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005262 calcium channel activity
GO:0005515 protein binding
GO:0031625 ubiquitin protein ligase binding
GO:0038023 signaling receptor activity
GO:0061630 ubiquitin protein ligase activity

Biological Process:
GO:0000082 G1/S transition of mitotic cell cycle
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006974 cellular response to DNA damage stimulus
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0016032 viral process
GO:0016567 protein ubiquitination
GO:0038128 ERBB2 signaling pathway
GO:0043687 post-translational protein modification
GO:0070588 calcium ion transmembrane transport
GO:0097193 intrinsic apoptotic signaling pathway

Cellular Component:
GO:0005829 cytosol
GO:0031461 cullin-RING ubiquitin ligase complex
GO:0031466 Cul5-RING ubiquitin ligase complex
GO:0080008 Cul4-RING E3 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK125294 - Homo sapiens cDNA FLJ43304 fis, clone NT2NE2005688, highly similar to Vasopressin-activated calcium-mobilizing receptor.
AK292575 - Homo sapiens cDNA FLJ75330 complete cds, highly similar to Homo sapiens cullin 5, mRNA.
BC063306 - Homo sapiens cullin 5, mRNA (cDNA clone MGC:71728 IMAGE:30331132), complete cds.
X81882 - H.sapiens mRNA for for vasopressin activated calcium mobilizing receptor-like protein.
BC017838 - Homo sapiens cDNA clone IMAGE:4666355, containing frame-shift errors.
CU687672 - Synthetic construct Homo sapiens gateway clone IMAGE:100020944 5' read CUL5 mRNA.
KJ897804 - Synthetic construct Homo sapiens clone ccsbBroadEn_07198 CUL5 gene, encodes complete protein.
AF327710 - Homo sapiens cullin 5 mRNA, complete cds.
AF017061 - Homo sapiens vasopressin-activated calcium mobilizing putative receptor protein (VACM-1) mRNA, complete cds.
JD437476 - Sequence 418500 from Patent EP1572962.
JD450933 - Sequence 431957 from Patent EP1572962.
JD313404 - Sequence 294428 from Patent EP1572962.
JD202764 - Sequence 183788 from Patent EP1572962.
BC037203 - Homo sapiens cDNA clone IMAGE:4820631.
JD491470 - Sequence 472494 from Patent EP1572962.
JD114530 - Sequence 95554 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04120 - Ubiquitin mediated proteolysis

Reactome (by CSHL, EBI, and GO)

Protein Q93034 (Reactome details) participates in the following event(s):

R-HSA-180555 Association of APOBEC3G:Vif with the Cul5-SCF complex
R-HSA-1918095 CUL5 mediates ubiquitination of ERBB2
R-HSA-180540 Multi-ubiquitination of APOBEC3G
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-8952039 NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex
R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8952044 AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-8863795 Downregulation of ERBB2 signaling
R-HSA-162909 Host Interactions of HIV factors
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-8951664 Neddylation
R-HSA-1227986 Signaling by ERBB2
R-HSA-162906 HIV Infection
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-597592 Post-translational protein modification
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-5663205 Infectious disease
R-HSA-1280218 Adaptive Immune System
R-HSA-392499 Metabolism of proteins
R-HSA-162582 Signal Transduction
R-HSA-1643685 Disease
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A8K960, CUL5_HUMAN, ENST00000393094.1, ENST00000393094.2, ENST00000393094.3, ENST00000393094.4, ENST00000393094.5, ENST00000393094.6, NM_003478, O14766, Q93034, Q9BZC6, uc001pjv.1, uc001pjv.2, uc001pjv.3, uc001pjv.4, uc001pjv.5, VACM1
UCSC ID: ENST00000393094.7
RefSeq Accession: NM_003478
Protein: Q93034 (aka CUL5_HUMAN)
CCDS: CCDS31668.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.