Human Gene NNMT (ENST00000535401.5) from GENCODE V44
  Description: Homo sapiens nicotinamide N-methyltransferase (NNMT), transcript variant 4, mRNA. (from RefSeq NM_001372047)
RefSeq Summary (NM_001372047): N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
Gencode Transcript: ENST00000535401.5
Gencode Gene: ENSG00000166741.8
Transcript (Including UTRs)
   Position: hg38 chr11:114,257,831-114,313,285 Size: 55,455 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg38 chr11:114,296,557-114,312,477 Size: 15,921 Coding Exon Count: 3 

Page IndexSequence and LinksPrimersMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:114,257,831-114,313,285)mRNA (may differ from genome)Protein (264 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCLynxMalacardsMGIOMIMPubMed
UniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NNMT
Diseases sorted by gene-association score: kidney cancer (2), parkinson disease, late-onset (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 346.95 RPKM in Liver
Total median expression: 2095.49 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -92.60264-0.351 Picture PostScript Text
3' UTR -241.10808-0.298 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR025818 - NNMT
IPR025820 - NNMT/PNMT/TEMT_CS
IPR000940 - NNMT_TEMT_trans

Pfam Domains:
PF01234 - NNMT/PNMT/TEMT family

ModBase Predicted Comparative 3D Structure on Q6FH49
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008112 nicotinamide N-methyltransferase activity
GO:0008168 methyltransferase activity
GO:0016740 transferase activity

Biological Process:
GO:0010243 response to organonitrogen compound
GO:0031100 animal organ regeneration
GO:0032259 methylation
GO:0042493 response to drug

Cellular Component:
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC000234 - Homo sapiens nicotinamide N-methyltransferase, mRNA (cDNA clone MGC:2121 IMAGE:3350358), complete cds.
AK026845 - Homo sapiens cDNA: FLJ23192 fis, clone REC00138, highly similar to HSU08021 Human nicotinamide N-methyltransferase (NNMT) mRNA.
U08021 - Human nicotinamide N-methyltransferase (NNMT) mRNA, complete cds.
JD375825 - Sequence 356849 from Patent EP1572962.
KJ897252 - Synthetic construct Homo sapiens clone ccsbBroadEn_06646 NNMT gene, encodes complete protein.
AB590349 - Synthetic construct DNA, clone: pFN21AE1345, Homo sapiens NNMT gene for nicotinamide N-methyltransferase, without stop codon, in Flexi system.
DQ892841 - Synthetic construct clone IMAGE:100005471; FLH190198.01X; RZPDo839F0875D nicotinamide N-methyltransferase (NNMT) gene, encodes complete protein.
DQ896088 - Synthetic construct Homo sapiens clone IMAGE:100010548; FLH190194.01L; RZPDo839F0865D nicotinamide N-methyltransferase (NNMT) gene, encodes complete protein.
CR541907 - Homo sapiens full open reading frame cDNA clone RZPDo834G0333D for gene NNMT, nicotinamide N-methyltransferase; complete cds, incl. stopcodon.
JD118670 - Sequence 99694 from Patent EP1572962.
JD478582 - Sequence 459606 from Patent EP1572962.
JD463830 - Sequence 444854 from Patent EP1572962.
JD148319 - Sequence 129343 from Patent EP1572962.
JD249865 - Sequence 230889 from Patent EP1572962.
JD249866 - Sequence 230890 from Patent EP1572962.
JD238297 - Sequence 219321 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00760 - Nicotinate and nicotinamide metabolism
hsa01100 - Metabolic pathways

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000535401.1, ENST00000535401.2, ENST00000535401.3, ENST00000535401.4, hCG_39357, NM_001372047, Q6FH49, Q6FH49_HUMAN, uc001por.1, uc001por.2
UCSC ID: ENST00000535401.5
RefSeq Accession: NM_001372047
Protein: Q6FH49 CCDS: CCDS8368.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.