Human Gene ETFBKMT (ENST00000395763.7) from GENCODE V44
  Description: Homo sapiens electron transfer flavoprotein subunit beta lysine methyltransferase (ETFBKMT), transcript variant 1, mRNA. (from RefSeq NM_173802)
Gencode Transcript: ENST00000395763.7
Gencode Gene: ENSG00000139160.13
Transcript (Including UTRs)
   Position: hg38 chr12:31,659,187-31,668,967 Size: 9,781 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr12:31,661,954-31,667,990 Size: 6,037 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:31,659,187-31,668,967)mRNA (may differ from genome)Protein (262 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
LynxMGIneXtProtOMIMPubMedReactome
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MET20_HUMAN
DESCRIPTION: RecName: Full=Methyltransferase-like protein 20; EC=2.1.1.-;
FUNCTION: Probable methyltransferase (By similarity).
SIMILARITY: Belongs to the methyltransferase superfamily. METTL20 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.51 RPKM in Adrenal Gland
Total median expression: 36.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -108.70289-0.376 Picture PostScript Text
3' UTR -254.70977-0.261 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010456 - Ribosomal-L11_MeTrfase_PrmA

Pfam Domains:
PF06325 - Ribosomal protein L11 methyltransferase (PrmA)

ModBase Predicted Comparative 3D Structure on Q8IXQ9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008168 methyltransferase activity
GO:0016279 protein-lysine N-methyltransferase activity
GO:0016740 transferase activity
GO:0031072 heat shock protein binding

Biological Process:
GO:0006479 protein methylation
GO:0018022 peptidyl-lysine methylation
GO:0018023 peptidyl-lysine trimethylation
GO:0032259 methylation
GO:1904733 negative regulation of electron carrier activity
GO:1904736 negative regulation of fatty acid beta-oxidation using acyl-CoA dehydrogenase

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AK290248 - Homo sapiens cDNA FLJ75625 complete cds.
BC039535 - Homo sapiens chromosome 12 open reading frame 72, mRNA (cDNA clone MGC:50559 IMAGE:5744381), complete cds.
BC039107 - Homo sapiens chromosome 12 open reading frame 72, mRNA (cDNA clone MGC:42963 IMAGE:4837913), complete cds.
AL832339 - Homo sapiens mRNA; cDNA DKFZp451L235 (from clone DKFZp451L235).
JD234680 - Sequence 215704 from Patent EP1572962.
CU691724 - Synthetic construct Homo sapiens gateway clone IMAGE:100021338 5' read MGC50559 mRNA.
HQ448646 - Synthetic construct Homo sapiens clone IMAGE:100072085; CCSB011360_01 hypothetical protein MGC50559 (MGC50559) gene, encodes complete protein.
KJ895902 - Synthetic construct Homo sapiens clone ccsbBroadEn_05296 METTL20 gene, encodes complete protein.
JD082058 - Sequence 63082 from Patent EP1572962.
BC039417 - Homo sapiens hypothetical protein MGC50559, mRNA (cDNA clone IMAGE:5302297).
JD093345 - Sequence 74369 from Patent EP1572962.
JD086741 - Sequence 67765 from Patent EP1572962.
JD327659 - Sequence 308683 from Patent EP1572962.
JD527736 - Sequence 508760 from Patent EP1572962.
JD541225 - Sequence 522249 from Patent EP1572962.
JD112734 - Sequence 93758 from Patent EP1572962.
JD228611 - Sequence 209635 from Patent EP1572962.
JD258856 - Sequence 239880 from Patent EP1572962.
JD152373 - Sequence 133397 from Patent EP1572962.
JD256860 - Sequence 237884 from Patent EP1572962.
JD407690 - Sequence 388714 from Patent EP1572962.
JD160470 - Sequence 141494 from Patent EP1572962.
JD069673 - Sequence 50697 from Patent EP1572962.
JD132290 - Sequence 113314 from Patent EP1572962.
JD149116 - Sequence 130140 from Patent EP1572962.
JD072430 - Sequence 53454 from Patent EP1572962.
JD331132 - Sequence 312156 from Patent EP1572962.
JD234610 - Sequence 215634 from Patent EP1572962.
JD107242 - Sequence 88266 from Patent EP1572962.
JD506283 - Sequence 487307 from Patent EP1572962.
JD318596 - Sequence 299620 from Patent EP1572962.
JD374341 - Sequence 355365 from Patent EP1572962.
JD232898 - Sequence 213922 from Patent EP1572962.
JD492107 - Sequence 473131 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8IXQ9 (Reactome details) participates in the following event(s):

R-HSA-8931858 ETFBKMT transfers 3xCH3 from 3xAdoMet to ETFB
R-HSA-8876725 Protein methylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: C12orf72, D3DUW3, ENST00000395763.1, ENST00000395763.2, ENST00000395763.3, ENST00000395763.4, ENST00000395763.5, ENST00000395763.6, MET20_HUMAN, METTL20, NM_173802, Q8IXQ9, uc001rkl.1, uc001rkl.2, uc001rkl.3, uc001rkl.4
UCSC ID: ENST00000395763.7
RefSeq Accession: NM_173802
Protein: Q8IXQ9 (aka MET20_HUMAN)
CCDS: CCDS8724.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.