Human Gene EEF1AKMT3 (ENST00000333012.5) from GENCODE V44
  Description: Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA. (from RefSeq NM_206914)
Gencode Transcript: ENST00000333012.5
Gencode Gene: ENSG00000123427.17
Transcript (Including UTRs)
   Position: hg38 chr12:57,772,678-57,782,541 Size: 9,864 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr12:57,772,725-57,780,276 Size: 7,552 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:57,772,678-57,782,541)mRNA (may differ from genome)Protein (149 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
LynxMGIneXtProtOMIMPubMedUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MT21B_HUMAN
DESCRIPTION: RecName: Full=Methyltransferase-like protein 21B; EC=2.1.1.-; AltName: Full=Hepatocellular carcinoma-associated antigen 557a;
FUNCTION: Probable methyltransferase (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the methyltransferase superfamily. METTL21 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.60 RPKM in Pancreas
Total median expression: 217.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.4047-0.413 Picture PostScript Text
3' UTR -693.002265-0.306 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019410 - Nicotinamide_N-MeTfrase-like

Pfam Domains:
PF10294 - Lysine methyltransferase

ModBase Predicted Comparative 3D Structure on Q96AZ1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0016279 protein-lysine N-methyltransferase activity
GO:0016740 transferase activity
GO:0031072 heat shock protein binding

Biological Process:
GO:0018022 peptidyl-lysine methylation
GO:0032259 methylation

Cellular Component:
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005856 cytoskeleton
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AF455816 - Homo sapiens hepatocellular carcinoma-associated antigen HCA557a mRNA, complete cds.
BC016395 - Homo sapiens family with sequence similarity 119, member B, mRNA (cDNA clone MGC:16818 IMAGE:3882414), complete cds.
BC099841 - Homo sapiens family with sequence similarity 119, member B, mRNA (cDNA clone MGC:119654 IMAGE:40010149), complete cds.
BC103501 - Homo sapiens family with sequence similarity 119, member B, transcript variant 1, mRNA (cDNA clone MGC:119656 IMAGE:40010152), complete cds.
AK292276 - Homo sapiens cDNA FLJ77367 complete cds, highly similar to Homo sapiens hepatocellularcarcinoma-associated antigen HCA557a, transcript variant 1, mRNA.
AK024983 - Homo sapiens cDNA: FLJ21330 fis, clone COL02466.
AL050100 - Homo sapiens mRNA; cDNA DKFZp586D0919 (from clone DKFZp586D0919).
CU677623 - Synthetic construct Homo sapiens gateway clone IMAGE:100016821 5' read FAM119B mRNA.
AB528834 - Synthetic construct DNA, clone: pF1KE0458, Homo sapiens FAM119B gene for family with sequence similarity 119, member B, without stop codon, in Flexi system.
AM393242 - Synthetic construct Homo sapiens clone IMAGE:100002383 for hypothetical protein (FAM119B gene).
AM393762 - Synthetic construct Homo sapiens clone IMAGE:100002384 for hypothetical protein (FAM119B gene).
KJ898564 - Synthetic construct Homo sapiens clone ccsbBroadEn_07958 METTL21B gene, encodes complete protein.
JD196598 - Sequence 177622 from Patent EP1572962.
JD138789 - Sequence 119813 from Patent EP1572962.
KM576716 - Homo sapiens clone METTL21B-TSFM_M2-T2-T5out mRNA sequence.
JD339970 - Sequence 320994 from Patent EP1572962.
JD471067 - Sequence 452091 from Patent EP1572962.
JD316972 - Sequence 297996 from Patent EP1572962.
JD099042 - Sequence 80066 from Patent EP1572962.
JD470264 - Sequence 451288 from Patent EP1572962.
JD438784 - Sequence 419808 from Patent EP1572962.
JD193296 - Sequence 174320 from Patent EP1572962.
JD326140 - Sequence 307164 from Patent EP1572962.
JD526711 - Sequence 507735 from Patent EP1572962.
JD159800 - Sequence 140824 from Patent EP1572962.
JD084340 - Sequence 65364 from Patent EP1572962.
JD317662 - Sequence 298686 from Patent EP1572962.
JD463241 - Sequence 444265 from Patent EP1572962.
JD338004 - Sequence 319028 from Patent EP1572962.
JD415738 - Sequence 396762 from Patent EP1572962.
JD072090 - Sequence 53114 from Patent EP1572962.
JD550372 - Sequence 531396 from Patent EP1572962.
JD293539 - Sequence 274563 from Patent EP1572962.
JD410584 - Sequence 391608 from Patent EP1572962.
JD227079 - Sequence 208103 from Patent EP1572962.
JD060974 - Sequence 41998 from Patent EP1572962.
JD300462 - Sequence 281486 from Patent EP1572962.
JD520946 - Sequence 501970 from Patent EP1572962.
JD233856 - Sequence 214880 from Patent EP1572962.
JD326761 - Sequence 307785 from Patent EP1572962.
JD325042 - Sequence 306066 from Patent EP1572962.
JD154277 - Sequence 135301 from Patent EP1572962.
JD305778 - Sequence 286802 from Patent EP1572962.
JD224878 - Sequence 205902 from Patent EP1572962.
JD147299 - Sequence 128323 from Patent EP1572962.
JD417379 - Sequence 398403 from Patent EP1572962.
JD244815 - Sequence 225839 from Patent EP1572962.
JD167411 - Sequence 148435 from Patent EP1572962.
JD565122 - Sequence 546146 from Patent EP1572962.
JD281751 - Sequence 262775 from Patent EP1572962.
JD519605 - Sequence 500629 from Patent EP1572962.
JD215212 - Sequence 196236 from Patent EP1572962.
JD053365 - Sequence 34389 from Patent EP1572962.
JD310321 - Sequence 291345 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000333012.1, ENST00000333012.2, ENST00000333012.3, ENST00000333012.4, FAM119B, HCA557A, METTL21B, MT21B_HUMAN, NM_206914, Q96AZ1, Q9H749, Q9Y3W2, uc001sqf.1, uc001sqf.2, uc001sqf.3, uc001sqf.4
UCSC ID: ENST00000333012.5
RefSeq Accession: NM_206914
Protein: Q96AZ1 (aka MT21B_HUMAN)
CCDS: CCDS31848.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.