Human Gene SERPINA12 (ENST00000341228.2) from GENCODE V44
  Description: Homo sapiens serpin family A member 12 (SERPINA12), transcript variant 1, mRNA. (from RefSeq NM_173850)
Gencode Transcript: ENST00000341228.2
Gencode Gene: ENSG00000165953.10
Transcript (Including UTRs)
   Position: hg38 chr14:94,487,283-94,517,844 Size: 30,562 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr14:94,487,303-94,498,397 Size: 11,095 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:94,487,283-94,517,844)mRNA (may differ from genome)Protein (414 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SPA12_HUMAN
DESCRIPTION: RecName: Full=Serpin A12; AltName: Full=OL-64; AltName: Full=Visceral adipose tissue-derived serine protease inhibitor; Short=Vaspin; AltName: Full=Visceral adipose-specific serpin; Flags: Precursor;
FUNCTION: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
SUBCELLULAR LOCATION: Secreted (Potential).
TISSUE SPECIFICITY: Expressed in visceral adipose tissues.
SIMILARITY: Belongs to the serpin family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: SERPINA12
Diseases sorted by gene-association score: obesity (2), diabetes mellitus, noninsulin-dependent (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 190.62 RPKM in Skin - Sun Exposed (Lower leg)
Total median expression: 320.27 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -269.50796-0.339 Picture PostScript Text
3' UTR -2.9020-0.145 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023796 - Serpin_dom
IPR000215 - Serpin_fam

Pfam Domains:
PF00079 - Serpin (serine protease inhibitor)

ModBase Predicted Comparative 3D Structure on Q8IW75
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0030414 peptidase inhibitor activity

Biological Process:
GO:0010466 negative regulation of peptidase activity
GO:0010951 negative regulation of endopeptidase activity
GO:0014068 positive regulation of phosphatidylinositol 3-kinase signaling
GO:0045721 negative regulation of gluconeogenesis
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0051055 negative regulation of lipid biosynthetic process
GO:0090181 regulation of cholesterol metabolic process
GO:0090207 regulation of triglyceride metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005886 plasma membrane


-  Descriptions from all associated GenBank mRNAs
  AY326420 - Homo sapiens visceral adipose-specific SERPIN mRNA, complete cds.
BC040857 - Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12, mRNA (cDNA clone MGC:50359 IMAGE:5527002), complete cds.
AY177692 - Homo sapiens OL-64 mRNA, complete cds.
HQ448570 - Synthetic construct Homo sapiens clone IMAGE:100072008; CCSB011349_01 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 (SERPINA12) gene, encodes complete protein.
KJ895582 - Synthetic construct Homo sapiens clone ccsbBroadEn_04976 SERPINA12 gene, encodes complete protein.
JD487912 - Sequence 468936 from Patent EP1572962.
CU691926 - Synthetic construct Homo sapiens gateway clone IMAGE:100021359 5' read SERPINA12 mRNA.
JD419021 - Sequence 400045 from Patent EP1572962.
JD527980 - Sequence 509004 from Patent EP1572962.
JD360053 - Sequence 341077 from Patent EP1572962.
JD560151 - Sequence 541175 from Patent EP1572962.
JD067213 - Sequence 48237 from Patent EP1572962.
JD176663 - Sequence 157687 from Patent EP1572962.
JD334724 - Sequence 315748 from Patent EP1572962.
JD436820 - Sequence 417844 from Patent EP1572962.
JD496648 - Sequence 477672 from Patent EP1572962.
JD148480 - Sequence 129504 from Patent EP1572962.
JD203529 - Sequence 184553 from Patent EP1572962.
JD068674 - Sequence 49698 from Patent EP1572962.
JD260019 - Sequence 241043 from Patent EP1572962.
JD096887 - Sequence 77911 from Patent EP1572962.
JD068199 - Sequence 49223 from Patent EP1572962.
JD383487 - Sequence 364511 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000341228.1, NM_173850, Q8IW75, SPA12_HUMAN, uc001ydj.1, uc001ydj.2, uc001ydj.3
UCSC ID: ENST00000341228.2
RefSeq Accession: NM_173850
Protein: Q8IW75 (aka SPA12_HUMAN)
CCDS: CCDS9926.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.