Human Gene CD276 (ENST00000318424.9) from GENCODE V44
  Description: Homo sapiens CD276 molecule (CD276), transcript variant 2, mRNA. (from RefSeq NM_025240)
RefSeq Summary (NM_025240): The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Gencode Transcript: ENST00000318424.9
Gencode Gene: ENSG00000103855.18
Transcript (Including UTRs)
   Position: hg38 chr15:73,684,281-73,714,509 Size: 30,229 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr15:73,699,640-73,712,956 Size: 13,317 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:73,684,281-73,714,509)mRNA (may differ from genome)Protein (316 aa)
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CD276_HUMAN
DESCRIPTION: RecName: Full=CD276 antigen; AltName: Full=4Ig-B7-H3; AltName: Full=B7 homolog 3; Short=B7-H3; AltName: Full=Costimulatory molecule; AltName: CD_antigen=CD276; Flags: Precursor;
FUNCTION: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
SUBUNIT: Interacts with TREML2 and this interaction enhances T- cell activation.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Probable).
TISSUE SPECIFICITY: Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
INDUCTION: By bacterial lipopolysaccharides (LPS) in monocytes and by ionomycin in T and B-lymphocytes. Up-regulated in cells mediating rejection of human transplants.
MISCELLANEOUS: B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)- C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.
SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family.
SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
SIMILARITY: Contains 2 Ig-like V-type (immunoglobulin-like) domains.
SEQUENCE CAUTION: Sequence=BAC11344.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CD276
Diseases sorted by gene-association score: neuroblastoma (2), acute cervicitis (1), lymphoproliferative syndrome 2 (1), ovarian cystic teratoma (1), juvenile pilocytic astrocytoma (1), immunodeficiency with hyper-igm, type 2 (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.35 RPKM in Cervix - Endocervix
Total median expression: 461.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -127.50234-0.545 Picture PostScript Text
3' UTR -559.401553-0.360 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013162 - CD80_C2-set
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR003599 - Ig_sub
IPR003598 - Ig_sub2
IPR013106 - Ig_V-set

Pfam Domains:
PF08205 - CD80-like C2-set immunoglobulin domain
PF07686 - Immunoglobulin V-set domain

ModBase Predicted Comparative 3D Structure on Q5ZPR3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005515 protein binding

Biological Process:
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0042102 positive regulation of T cell proliferation
GO:0042110 T cell activation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0050776 regulation of immune response

Cellular Component:
GO:0009897 external side of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AJ583696 - Homo sapiens 5'UTR for B7-H3 gene.
BC062581 - Homo sapiens CD276 molecule, mRNA (cDNA clone MGC:74893 IMAGE:5201491), complete cds.
AY358343 - Homo sapiens clone DNA41386 B7 hlog (UNQ309) mRNA, complete cds.
AK298522 - Homo sapiens cDNA FLJ58033 complete cds, highly similar to CD276 antigen precursor.
AK075549 - Homo sapiens cDNA PSEC0249 fis, clone NT2RP3001861, highly similar to B7 homolog 3.
AK292711 - Homo sapiens cDNA FLJ78024 complete cds.
AK074849 - Homo sapiens cDNA FLJ90368 fis, clone NT2RP2004205, highly similar to B7 homolog 3.
AK296358 - Homo sapiens cDNA FLJ61301 complete cds, highly similar to CD276 antigen precursor.
AK309728 - Homo sapiens cDNA, FLJ99769.
AK291721 - Homo sapiens cDNA FLJ78639 complete cds.
AJ583695 - Homo sapiens mRNA for B7-H3 protein.
AX047072 - Sequence 5 from Patent WO0068266.
AF302102 - Homo sapiens costimulatory molecule mRNA, complete cds.
KJ905554 - Synthetic construct Homo sapiens clone ccsbBroadEn_15170 CD276-like gene, encodes complete protein.
AK074997 - Homo sapiens cDNA FLJ90516 fis, clone NT2RP3004481, highly similar to B7 homolog 3.
AL360144 - Homo sapiens EST from clone 943301, 3' end.
AL360136 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 2176457.
BC011578 - Homo sapiens CD276 molecule, mRNA (cDNA clone IMAGE:4548461).

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04514 - Cell adhesion molecules (CAMs)

-  Other Names for This Gene
  Alternate Gene Symbols: B7H3, CD276_HUMAN, ENST00000318424.1, ENST00000318424.2, ENST00000318424.3, ENST00000318424.4, ENST00000318424.5, ENST00000318424.6, ENST00000318424.7, ENST00000318424.8, NM_025240, PSEC0249, Q5ZPR3, Q6P5Y4, Q6UXI2, Q8NBI8, Q8NC34, Q8NCB6, Q9BXR1, uc002avw.1, uc002avw.2, UNQ309/PRO352
UCSC ID: ENST00000318424.9
RefSeq Accession: NM_025240
Protein: Q5ZPR3 (aka CD276_HUMAN)
CCDS: CCDS10251.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.