Human Gene RNPS1 (ENST00000566458.5) from GENCODE V44
Description: Homo sapiens RNA binding protein with serine rich domain 1 (RNPS1), transcript variant 4, mRNA. (from RefSeq NM_001286626) RefSeq Summary (NM_001286626): This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]. Gencode Transcript: ENST00000566458.5 Gencode Gene: ENSG00000205937.12 Transcript (Including UTRs) Position: hg38 chr16:2,253,117-2,268,397 Size: 15,281 Total Exon Count: 7 Strand: - Coding Region Position: hg38 chr16:2,253,964-2,268,056 Size: 14,093 Coding Exon Count: 7
ID:RNPS1_HUMAN DESCRIPTION: RecName: Full=RNA-binding protein with serine-rich domain 1; AltName: Full=SR-related protein LDC2; FUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Part of pre- and post- splicing multiprotein mRNP complexes. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Participates in mRNA 3'-end cleavage. Involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions. SUBUNIT: Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, PNN, RBM8A, RNPS1, SAP18 and ALYREF/THOC4. Forms heterodimers with ACIN1. Found in a heterotrimeric complex with ACIN1, RNPS1 and SAP18. Component of the active spliceosome. Associates with polysomes. Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with the cleaved p110 isoform of CDC2L1, CSNK2A1, PNN, SART3, SRP54, SRRM1 and TRA2B/SFRS10. INTERACTION: Q15020:SART3; NbExp=5; IntAct=EBI-395959, EBI-308619; SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylated on one or more of the four Ser/Thr residues (Ser-43, Thr-49, Ser-52 or Ser-53). Ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro. SIMILARITY: Belongs to the splicing factor SR family. SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q15287
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC108316 - Homo sapiens RNA binding protein S1, serine-rich domain, mRNA (cDNA clone MGC:117332 IMAGE:6151864), complete cds. AF274003 - Homo sapiens splicing-related factor RNPS1 (RNPS1) mRNA, complete cds, alternatively spliced. AF015608 - Homo sapiens SR protein (RNPS1) mRNA, complete cds. BC001659 - Homo sapiens RNA binding protein S1, serine-rich domain, mRNA (cDNA clone MGC:1125 IMAGE:2959954), complete cds. BC001838 - Homo sapiens RNA binding protein S1, serine-rich domain, mRNA (cDNA clone MGC:4450 IMAGE:2959954), complete cds. AK091886 - Homo sapiens cDNA FLJ34567 fis, clone KIDNE2007954. AK092131 - Homo sapiens cDNA FLJ34812 fis, clone NT2NE2007365. AX747369 - Sequence 894 from Patent EP1308459. L37368 - Human (clone E5.1) RNA-binding protein mRNA, complete cds. JD288130 - Sequence 269154 from Patent EP1572962. JD422489 - Sequence 403513 from Patent EP1572962. JD541858 - Sequence 522882 from Patent EP1572962. JD168519 - Sequence 149543 from Patent EP1572962. JD451802 - Sequence 432826 from Patent EP1572962. JD500939 - Sequence 481963 from Patent EP1572962. AK316132 - Homo sapiens cDNA, FLJ79031 complete cds, highly similar to RNA-binding protein with serine-rich domain 1. JD526102 - Sequence 507126 from Patent EP1572962. AF247662 - Homo sapiens SR-related protein LD2 mRNA, partial cds. JD338742 - Sequence 319766 from Patent EP1572962. JD338743 - Sequence 319767 from Patent EP1572962. JD470966 - Sequence 451990 from Patent EP1572962. JD039115 - Sequence 20139 from Patent EP1572962. JD454198 - Sequence 435222 from Patent EP1572962. JD476404 - Sequence 457428 from Patent EP1572962. JD476405 - Sequence 457429 from Patent EP1572962. JD482396 - Sequence 463420 from Patent EP1572962. AK293343 - Homo sapiens cDNA FLJ51689 complete cds, highly similar to RNA-binding protein with serine-rich domain 1. JD165573 - Sequence 146597 from Patent EP1572962. JD396369 - Sequence 377393 from Patent EP1572962. AK303100 - Homo sapiens cDNA FLJ51419 complete cds, highly similar to RNA-binding protein with serine-rich domain 1. JD054877 - Sequence 35901 from Patent EP1572962. JD068622 - Sequence 49646 from Patent EP1572962. JD068623 - Sequence 49647 from Patent EP1572962. AK289955 - Homo sapiens cDNA FLJ75076 complete cds, highly similar to Homo sapiens RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 2, mRNA. JD258709 - Sequence 239733 from Patent EP1572962. AB445101 - Homo sapiens rnps1 mRNA for RNA binding protein S1, complete cds. EU176344 - Synthetic construct Homo sapiens clone IMAGE:100006453; FLH185154.01X; RZPDo839B09251D RNA binding protein S1, serine-rich domain (RNPS1) gene, encodes complete protein. KJ893166 - Synthetic construct Homo sapiens clone ccsbBroadEn_02560 RNPS1 gene, encodes complete protein. DQ895514 - Synthetic construct Homo sapiens clone IMAGE:100009974; FLH185147.01L; RZPDo839F10145D RNA binding protein S1, serine-rich domain (RNPS1) gene, encodes complete protein. AB464183 - Synthetic construct DNA, clone: pF1KB8281, Homo sapiens RNPS1 gene for RNA binding protein S1, serine-rich domain, without stop codon, in Flexi system. DQ574166 - Homo sapiens piRNA piR-42278, complete sequence. DQ598424 - Homo sapiens piRNA piR-36490, complete sequence. JD068591 - Sequence 49615 from Patent EP1572962. JD479472 - Sequence 460496 from Patent EP1572962. JD462764 - Sequence 443788 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q15287 (Reactome details) participates in the following event(s):
R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-72185 mRNA polyadenylation R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-159101 NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B) R-HSA-927832 UPF1 binds an mRNP with a termination codon preceding an Exon Junction Complex R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC R-HSA-927889 SMG1 phosphorylates UPF1 (enhanced by Exon Junction Complex) R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72172 mRNA Splicing R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-72187 mRNA 3'-end processing R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) R-HSA-376176 Signaling by ROBO receptors R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-8953854 Metabolism of RNA R-HSA-927802 Nonsense-Mediated Decay (NMD) R-HSA-422475 Axon guidance R-HSA-73857 RNA Polymerase II Transcription R-HSA-1266738 Developmental Biology R-HSA-74160 Gene expression (Transcription)
US Server
